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PCR–RFLP identification of endemic Chilean species of Rhagoletis (Diptera: Tephritidae) attacking Solanaceae

Published online by Cambridge University Press:  09 March 2007

M. Salazar ,
C. Theoduloz ,
A. Vega ,
F. Poblete ,
E. González ,
R. Badilla  and
L. Meza-Basso
M. Salazar
Affiliation:
Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, TalcaChile
C. Theoduloz
Affiliation:
Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, TalcaChile
A. Vega
Affiliation:
Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, TalcaChile
F. Poblete
Affiliation:
Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, TalcaChile
E. González
Affiliation:
Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, TalcaChile
R. Badilla
Affiliation:
Servicio Agrícola y Ganadero, VII Región, TalcaChile
L. Meza-Basso*
Affiliation:
Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, TalcaChile
*
*Fax: 56 71 200279 E-mail: mezabas@pehuenche.utalca.cl.
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Abstract

Four species of the genus Rhagoletis are native to Chile: R. nova (Schiner), R. conversa, (Brèthes), R. penela Foote and R. tomatis Foote. Currently, identification of these species is based on morphological criteria, but their strong similarity makes precise recognition difficult. To clarify species separation for quarantine purposes, a reliable method based on a PCR–RFLP procedure is reported. A DNA region containing mitochondrial NADH dehydrogenase genes was selected as a target sequence for the analysis. The amplification products (c. 1 kb) were digested with either SspI or DdeI, yielding specific patterns that differentiated each of the endemic species. Complete nucleotide sequences were determined, confirming empirical restriction maps. This report updates information on the geographical distribution of Rhagoletis species in Chile.

Type
Research Article
Information
Bulletin of Entomological Research , Volume 92 , Issue 4 , August 2002 , pp. 337 - 341
Copyright
Copyright © Cambridge University Press 2002

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References

Aikhionbare, F.O. & Mayo, Z.B. (2000) Mitochondrial DNA sequences of greenbug (Homoptera: Aphididae) biotypes. Biomolecular Engineering 16, 199–205.Google Scholar
Benecke, M. & Wells, J. (2000) DNA techniques for forensic entomology. In Forensic entomology: the utility of arthropods in legal investigation. pp 341–352. [Byrd, J.H. & Castner, J.L., editors]. Washington, DC: CRC Press.Google Scholar
Boller, E.F. & Prokopy, R.J. (1976) Bionomics and management of Rhagoletis. Annual Review of Entomology 21, 223–246.Google Scholar
Caterino, M.S., Cho, S. & Sperling, F.A.H. (2000) The current state of insect molecular systematics: a thriving tower of Babel. Annual Review of Entomology 45, 1–54.Google Scholar
Clary, D.O. & Wolstenholme, D.R. (1985) The mitochondrial DNA molecule of Drosophila yakuba: nucleotide sequence, gene organization, and genetic code. Journal of Molecular Evolution 22, 252–271.Google Scholar
Foote, R. (1981) The genus Rhagoletis Loew South of United States. In Diptera: Tephritidae. pp. US Department of Agriculture Technical Bulletin 1607: 75 pp.Google Scholar
Frías, L.D. (1992) Aspectos de la biología evolutiva de especies de Tephritidae (Diptera) de distribución geográfica. Acta Entomologica Chilena 17, 69–79.Google Scholar
Frías, L.D. & Martínez, H. (1991) Estudio taxonómico en Rhagoletis tomatis (Foote) (Diptera: Tephritidae). Acta Entomologica Chilena 16, 247–254.Google Scholar
Frías, L.D., González, C., Henry, A. & Alviña, A. (1993) Distribución geográfica y respuesta visual de Rhagoletis tomatis Foote (Diptera: Tephritidae) a trampas esféricas y rectangulares de diferentes colores. Acta Entomologica Chilena 18, 185–194.Google Scholar
Gasparich, G.E., Sheppard, W.S., Han, H.-Y., McPheron, B.A. & Steck, G.J. (1995) Analysis of mitochondrial DNA and development of PCR-based diagnostic molecular markers for Mediterranean fruit fly (Ceratitis capitata) populations. Insect Molecular Biology 4, 61–67.Google Scholar
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium, Series 41, 95–98.Google Scholar
Han, H.-Y. & McPheron, B.A. (1997) Molecular phylogenetic study of Tephritidae (Insecta: Diptera) using partial sequences of the mitochondrial 16S ribosomal DNA. Molecular Phylogenetics and Evolution 7, 17–32.CrossRefGoogle ScholarPubMed
Koekemoer, L.L., Coetzee, M. & Hunt, R.H. (1998) HpaII endonuclease distinguishes two species in the Anopheles funestus group. Insect Molecular Biology 7, 273–277.CrossRefGoogle ScholarPubMed
Lessinger, A.C., Martins Junqueira, A.C., Lemos, T.A., Kemper, E.L., Da Silva, F.R., Vettore, A.L., Arruda, P. & Azeredo-Espin, A.M. (2000) The mitochondrial genome of the primary screwworm fly Cochliomyia hominivorax. Insect Molecular Biology 9, 521–529.CrossRefGoogle ScholarPubMed
Loxdale, H.D. & Lushai, G. (1998) Molecular markers in entomology. Bulletin of Entomological Research 88, 577–600.Google Scholar
Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) In Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor: New York.Google Scholar
Sanger, F., Nicklen, S. & Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74, 5463–5467.CrossRefGoogle ScholarPubMed
Smith, J.J. & Bush, G.L. (1997) Phylogeny of the genus Rhagoletis (Diptera: Tephritidae) inferred from DNA sequences of mitochondrial cytochrome oxidase II. Molecular Phylogenetics and Evolution 7, 33–43.Google Scholar
Spanos, L., Koutroumbas, G., Kotsyfakis, M. & Louis, C. (2000) The mitochondrial genome of the Mediterranean fruit fly, Ceratitis capitata. Insect Molecular Biology 9, 139–144.CrossRefGoogle ScholarPubMed
Taylor, D.B., Szalanski, A.L. & Peterson, R.D. (1996) Identification of screwworm species by polymerase chain reaction–restriction fragment length polymorphism. Medical and Veterinary Entomology 10, 63–70.CrossRefGoogle ScholarPubMed
Thompson, F.C. (1999) In Fruit fly expert system and systematic information database. Myia (1998) 524 pp.Google Scholar
Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence aligment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673–4680.Google Scholar
Van Bortel, W., Trung, H.D., Roelants, P., Harbach, R.E., Bacheljau, T. & Coosemans, M. (2000) Molecular identification of Anopheles minimus s.l. beyond distinguishing the members of the species complex. Insect Molecular Biology 9, 335–340.Google Scholar