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Similarity in oviposition aggregation pheromone composition within the Simulium damnosum (Diptera: Simuliidae) species complex

  • P. J. McCall (a1), M. D. Wilson (a2), B. D. Dueben (a3), B. M. de Clare Bronsvoort (a1) and R. R. Heath (a3)...

The oviposition aggregation pheromone of six species or forms of the Simulium damnosum Theobald complex (Diptera: Simuliidae) from different sites in West Africa, was investigated by gas chromatographic analysis of hexane extracts of the ovaries from wild-caught flies, bloodfed and maintained until gravid in the laboratory. The two compounds previously shown to be released from fresh eggs and associated with mediation of oviposition aggregation were found in S. leonense Boakye, Post & Mosha (Sierra Leone), S. yahense Vajime & Dunbar (Ghana), S. sanctipauli Vajime & Dunbar (Ghana), S. sqnamosum; Enderlein (Cameroon), S. sirbanum Vajime & Dunbar (Ghana) and the Bioko form (island of Bioko, Equatorial Guinea). Coupled gas chromatography–mass spectrometry of the S. leonense, S. sanctipauli and the Bioko form extracts showed the two compounds to be identical in all three. Volatile emissions from freshly laid eggs of S. sanctipauli were similar to those previously described from S. leonense, and identical in chemical composition to gravid ovaries. No new compounds were detected in any ovary extracts or volatile emissions examined, demonstrating that the composition of the aggregation pheromone is similar throughout the S. damnosum species complex. Analysis of S. leonense adults of different age groups and physiological states showed that the compounds are detectable only in gravid ovaries at 2 or more days following the bloodmeal, suggesting that production of the pheromone occurs during egg development. Demonstration of an oviposition aggregation pheromone common throughout the species complex raises the possibility of developing an odour-baited trapping system for blackflies, which could be employed across the large area of Africa where they are the vectors of onchocerciasis.

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*International Institute of Parasitology, 395A Hatfield Road, St Albans, Herts, AL4 0XU, UK.
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R.H.A. Baker , P. Guillet , A. Sékétéli , P. Poudiougo , D. Boakye , M.D. Wilson & Y. Bissan (1990) Progress in controlling the reinvasion of windborne vectors into the western area of the Onchocerciasis Control Programme in West Africa. Philosophical Transactions of the Royal Society of London, B328, 731750.

R.J. Bartelt , A.M. Schaner & L.L. Jackson (1985) cis-vaccenyl acetate as an aggregation pheromone in Drosophila melanogaster. Journal of Chemical Ecology 11, 17471756.

R.J. Bartelt , A.M. Schaner & L.L. Jackson (1986) Aggregation pheromones in five taxa ot the Drosophila virilis species group. Physiological Entomology 11, 367376.

D.W. Bruno & B.R. Laurence (1979) The influence of the apical droplet of Culex egg rafts on oviposition of Culex pipiens fatigans (Diptera: Culicidae). Journal of Medical Entomology 16, 300305.

R.A. Cheke , P.J. McCall & J. Mas (1997) The vector status of the Bioko form of Simulium damnosum. Transactions of the Royal Society of Tropical Medicine and Hygiene 91. 153154.

J.B. Coupland (1991) Oviposition response of Simulium reptans (Diptera: Simuliidae) to the presence of conspecific eggs. Ecological Enotomology 16, 1115.

J.B. Davies (1962) Egg-laying habits of Simulium damnosum Theobald and Simulium medusaeforme from hargrevesi Gibbons in Northern Nigeria. Nature 196, 149150.

M.J. Dougherty , R.D. Ward & J.G.C. Hamilton (1992) Evidence for the accessory glands as the site of production of the oviposition attractant and/or stimulant of Lutzomyia longipalpis (Diptera: Psychodidae). Journal of Chemical Ecology 18, 11651175.

M.J. Dougherty , J.G.C. Hamilton & R.D. Ward (1994) Isolation of oviposition pheromone from the eggs of the sandfly Lutzomyia longipalpis. Medical and Veterinary Entomology, 8, 119124.

D.L. Faustini , W.L. Giese , J.K. Philips , & W.E. Burkholder (1982) Aggregation pheromone of the male granary weevil, Sitophilus granarius (L.). Journal of Chemical Ecology 8, 679687.

J.J. Grob (1982) Band boadening in space and the retention gap in capillary gas chromotography. Journal of Chromotography 237, 1523.

N.L. Hywel-Jones & M. Ladle (1986) Ovipositional behaviour of Simulium argyreatum and S. variegatum and its relationship to infection by the fungus Erynia conica (Entomophthoraceae). Freshwater Biology 16, 397403.

D.G. James , K. Mori , J.R. Aldrich & J.E. Oliver (1994) Flight-mediated attraction of Biprorulus bibax Breddin (Hemiptera: Pentatomidae) to natural and synthetic aggregation pheromone. Journal of Chemical Ecology 20, 7180.

P.J. McCall (1995) Oviposition pheromone in the Simulium damnosum complex. Medical and Veterinary Entomology 9, 101108.

P.J. McCall & M.M. Cameron (1995) Oviposition pheromones in insect vectors. Parasitology Today 11, 352355.

P.J. McCall , A.J. Trees , J.F. Walsh & D.H. Molyneux (1994) Aggregated ovipostionin the Simulium damnosum complex is mediated by eggs in a laboratory bioassay. Medical and Veterinary Entomology 8, 7680.

P.J. McCall , R.R. Heath , B.D. Dueben & M.D. Wilson (1997) Oviposition pheromone in the Simulium damnosum complex: biological activity of chemical fractions from gravid ovaries. Physiological Entomology, in press.

R.C. Muirhead-Thompson (1956) Communal oviposition in Simulium damnosum Theobald (Diptera: Simuliidae). Nature 178, 12971299.

D. Rochat , C. Malosse , M. Lettere , P. Ducrot , P. Zagatti , M. Renou & C. Descoins (1991) Male-produces aggregation pheromone of the American palm weevil, Rhynchophorus palmarum (L.) (Coleoptera, Curculionidae): collection, identification, electrophsiological activity, and laboratory bioassay. Journal of Chemical Ecology 17, 21272141.

R.K. Saini , A. Hassanali , J. Andoke , P. Ahuya & W.P. Ouma (1996) Identification of major components of larviposition pheromone from larvae of tsetse flies Glossina morsitans morsitans Westwood and Glossina morsitans centralis Machado. Jouranal of Chemical Ecology 22, 12111220.

C.A. Walgenbach , J.K. Phillips , D.L. Faustini & W.E. Burkholder (1983) Male-produced aggregation pheromone of the maize weevil, Sitophilus zeamais, and interspecific attraction between three Sitophilus species. Journal of Chemical Ecology 9, 831841.

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Bulletin of Entomological Research
  • ISSN: 0007-4853
  • EISSN: 1475-2670
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