Hostname: page-component-76fb5796d-9pm4c Total loading time: 0 Render date: 2024-04-29T10:58:45.887Z Has data issue: false hasContentIssue false
  • English
  • Français

A simple method for marking haematophagous insects during the act of feeding

Published online by Cambridge University Press:  10 July 2009

R. H. Knight  and
H. A. W. Southon
R. H. Knight
Affiliation:
East African Trypanosomiasis Research Organization, Tororo, Uganda.
H. A. W. Southon
Affiliation:
East African Trypanosomiasis Research Organization, Tororo, Uganda.
Get access Rights & Permissions [Opens in a new window]

Extract

Blood-meals of two species of haematophagous insects, Glossina morsitans Westw. and Aedes (Stegomyia) aegypti (L.), have been ‘ marked ’ by allowing groups of adults to feed on an ox to which an aqueous solution containing 4 g. of trypan blue had previously been administered intravenously. The dyestuff is detected by a paper chromatographic procedure in which the blood-meal, mixed with 0·1N NaOH, is applied to a narrow strip of Whatman No. 1 chromatographic filter paper and the chromatogram is developed in 0·1N NaOH. Under these conditions, trypan blue remains at the origin, whereas the other coloured components of the blood-meal move away from the point of application. The duration of the mark in the ox and in the insects has been examined. The trypan blue was detected in four out of six fresh blood-meals of Glossina taken 38 days after injection of the dye into the ox, in six out of six examples of Glossina examined two days after blood-meals taken 24 days after the injection, in four out of six examples of Glossina examined eight days after blood-meals taken immediately following the injection, in six out of six examples of Aedes one day after blood-meals taken 14 days after the injection and in six out of six examples of Aedes two days after blood-meals taken immediately following the injection.

Type
Research Paper
Information
Bulletin of Entomological Research , Volume 54 , Issue 3 , November 1963 , pp. 379 - 382
Copyright
Copyright © Cambridge University Press 1963

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Allison, A. C. (1960). Simple methods for identifying hosts of blood-feeding insects.—Trans. R. Soc. trap. Med. Hyg. 54 p. 295.Google Scholar
Cunningham, M. P., Harley, J. M. B. & Grainge, E. B. (1963). The labelling of animals with specific agglutinins and the detection of these agglutinins in the blood meals of Glossina.—Rep. E. Afr. Tryp. Res. Org. 1961 pp. 23–24.Google Scholar
Jackson, C. H. N. (1933). On a method of marking tsetse flies.—J. Anim. Ecol. 2 pp. 289–290.CrossRefGoogle Scholar
Jackson, C. H. N. (1953). A mixed population of Glossina morsitans and G. swynnertoni.—J. Anim. Ecol. 22 pp. 78–86.CrossRefGoogle Scholar
Yates, W. W., Gjullin, C. M., Lindquist, A. W. & Butts, J. S. (1951). Treatment of mosquito larvae and adults with radioactive phosphorus.—J. econ. Ent. 44 pp. 34–37.CrossRefGoogle Scholar