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Wing morphometry as a tool for correct identification of primary and secondary New World screwworm fly

  • M.L. Lyra (a1) (a2), L.M. Hatadani (a2), A.M.L. de Azeredo-Espin (a1) (a2) and L.B. Klaczko (a2)

Cochliomyia hominivorax and Cochliomyia macellaria are endemic Neotropical Calliphoridae species. The former causes severe myiasis in hosts while the latter is Sarcosaprophagous, but commonly found as a second invader in wounds. Due to the morphological similarity between them and the potential losses that C. hominivorax represents for cattle breeders, the rapid and correct identification of these two species is very important. In addition to a correct identification of these species, a good knowledge of C. hominivorax biology can be helpful for designing control programs. We applied geometric morphometric methods to assess wing differences between C. hominivorax and C. macellaria and conduct a preliminary analysis of wing morphological variation in C. hominivorax populations. Canonical variate analysis, using wing shape data, correctly classified 100% of the individuals analyzed according to sex and species. This result demonstrates that wing morphometry is a simple and reliable method for identifying C. hominivorax and C. macellaria samples and can be used to monitor C. hominivorax. Both species show sexual dimorphism, but in C. hominivorax it is magnified. We suggest that this may reflect different histories of selection pressures operating on males and females. Significant differences in wing size and shape were obtained among C. hominivorax populations, with little correlation with latitude. This result suggests that wing variation is also a good morphological marker for studying population variation in C. hominivorax.

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Andersson, M.B. (1994) Sexual Selection. 624 pp. Princeton, N, Princeton University Press.
Azeredo-Espin, A.M.L. (1987) Análise cariotípica, morfométrica e de compatibilidade sexual em linhagens brasileiras de Cochliomyia hominivorax (Diptera: Calliphoridae). PhD dissertation, University of Campinas (UNICAMP), Campinas, SP, Brazil.
Baumgartner, D.L. & Greenberg, B. (1985) Distribution and medical ecology of the blow flies (Diptera: Calliphoridae) of Peru. Annals of the Entomological Society of America 78, 565587.
Bitner-Mathé, B.C. & Klaczko, L.B. (1999) Plasticity of Drosophila melanogaster wing morphology: effects of sex, temperature and density. Genetica 105, 203210.
Bitner-Mathé, B.C., Peixoto, A.A. & Klaczko, L.B. (1995) Morphological variation in natural population of Drosophila mediopunctara: altitudinal cline, temporal changes and influence chromosome inversions. Heredity 75, 5461.
Bonduriansky, R. (2006) Convergent Evolution of Sexual Shape Dimorphism in Diptera. Journal of Morphology 267, 602611.
Bookstein, F.L. (1991) Morphometric Tools for Landmark Data. Cambridge, UK, Cambridge University Press.
Collinge, J.E., Hoffmann, A.A. & McKechnie, S.W. (2006) Altitudinal patterns for latitudinally varying traits and polymorphic markers in Drosophila melanogaster from eastern Australia. Journal of Evolutionary Biology 19(2), 473482.
Crystal, M.M. (1967) Reprodutive behavior of laboratory reared screwworm flies (Diptera: Calliphoridae). Journal of Medical Entomology 4, 443450.
Darwin, C. (1871) The Descent of Man and Selection in Relation to Sex. 620 pp. London, UK, John Murray.
David, J.R., Gibert, P., Mignon-Grasteau, S., Legout, H., Pétavy, G., Beaumont, C. & Moreteau, B. (2003) Genetic variability of sexual size dimorphism in natural population of Drosophila melanogaster: an isofemale-line approach. Journal of Genetics 82(3), 7988.
David, J.R., Araripe, L.O., Bitner-Mathé, B.C., Capy, P., Goñi, B., Klaczko, L.B., Legout, H., Martins, M.B., Vouidibio, J., Yassin, A. & Moreteau, B. (2006a) Sexual dimorphism of body size and sternopleural bristle number: a comparison of geographic populations of an invasive cosmopolitan dosophilid. Genetica 128, 109122.
David, J.R., Legout, H. & Moreteau, B. (2006b) Phenotypic plasticity of body size in a temperate population of Drosophila melanogaster: when the temperature-size rule does not apply. Journal of Genetics 85, 923.
De León, D. & Fox, I. (1980) Canine minima myiasis in Puerto Rico – a case report. Journal of Agriculture of University of Puerto Rico 64, 126128.
Dear, J.P. (1985) A revision of New World Crysomiini (Diptera: Calliphoridae). Revista Brasileira de Zoologia 3, 109169.
Dujardin, J.-P., Le Pont, F. & Baylac, M. (2003) Geographical versus interspecific differentiation of sand flies (Diptera: Psychodidae): a landmark data analysis. Bulletin of Entomological Research 93, 8790.
Fairbairn, D.J. (1997) Allometry for sexual size dimorphism: patterns and process in coevolution of body size in males and females. Annual Review of Ecology and Systematic 28, 659687.
FAO: Food and Agriculture Organization of United Nations (1993) Manual for the Control of the Screwworm Fly Cochliomyia hominivorax (Coquerel), vol. 2. Guide for the identification of flies in the genus Cochliomyia (Diptera: Calliphoridae). 18 pp. Rome, Italy, FAO.
Gagné, R.J. & Peterson, R.D. II (1982) Physical changes in the genitalia of males of the screwworm, Cochliomyia hominivorax (Diptera: Calliphoridae), caused by mating. Annals of the Entomological Society of America 75, 574578.
Gilchrist, G.W., Huey, R.B., Balanya, J., Pascual, M. & Serra, L. (2004) Time series of evolution in action: a latitudinal cline in wing size in South American Drosophila subosbscura. Evolution 58(4), 768780.
Greenberg, B., Varela, C., Bornstein, A. & Hernandez, H. (1963) Salmonellae from flies in a Mexican slaughterhouse. American Journal of Hygiene 77, 177183.
Guillot, F.S., Brown, H.E. & Broce, A.B. (1978) Behavior of sexually active male screwworm flies. Annals of the Entomological Society of America 71(2), 199201.
Guimarães, J.H. & Papavero, N. (1999) Myiasis in Man and Animals in the Neotropical Region. 308 pp. São Paulo, Brazil, Plêiade/FAPESP.
Hatadani, L.M. & Klaczko, L.B. (2008) Shape and size variation on the wing of Drosophila mediopunctata: influence of chromosome inversions and genotype-environment interaction. Genetica 133, 335342.
Hoffmann, A.A. & Shirriffs, J. (2002) Geographic variation for wing shape in Drosophila serrata. Evolution 56(5), 10681073.
Houle, D., Mezey, J., Galpern, P. & Carter, A. (2003) Automated measurement of Drosophila wings. BMC Evolutionary Biology 3, 2537.
Huey, R.B., Moreteau, B., Moreteau, J.C., Gibert, P., Gilchrist, G.W., Ives, A.R., Garland, T. Jr. & David, J.R. (2006) Sexual size dimorphism in a Drosophila clade, the D. obscura group. Zoology 109, 318330.
IAEA/FAO: International Atomic Energy Agency/Food and Agriculture Organization (2000) Genetic sexing and population genetics of screwworms. 31 pp. International Atomic Energy Agency, 7–11 August 2000, Vienna, Austria.
Imasheva, A.G., Bubli, O.A. & Lazebny, O.E. (1994) Variation in wing length in Eurasian natural populations of Drosophila melanogaster. Heredity 72, 508514.
Imasheva, A.G., Moreteau, B. & David, J.R. (2000) Growth temperature and genetic variability of wing dimensions in Drosophila: opposite trends in two sibling species. Genetical Research 76, 237247.
Infante-Vargas, M.E. & Azeredo-Espin, A.M.L. (1995) Genetic Variability in Mitochondrial DNA of Screwworm Cochliomyia hominivorax (Diptera: Calliphoridae) from Brazil. Biochemical Genetics 33, 737756.
James, A.C., Azevedo, R.B.R. & Partridge, L. (1995) Cellular basis and developmental timing in a size cline of Drosophila melanogaster. Genetics 140, 659666.
Josephson, R.L. & Krajden, S. (1993) An unusual nosocomial infection: nasotracheal myiasis. Journal of Otolaryngology 22, 4647.
Krafsur, E.S. (1978) Aggregation of male screwworm flies, Cochliomyia hominivorax (Coquerel) in South Texas (Diptera-Calliphoridae). Proceedings of the Entomological Society of Washington 80(2), 164170.
Krafsur, E.S. (2005) Role of population genetics in the sterile insect technique. pp. 389406in Dyck, V.A., Hendrichs, J. & Robinson, A.S. (Eds) Sterile Insect Technique: Principles and Practice in Area-Wide Integrated Pest Management. Dordrecht, The Netherlands, Springer.
Lande, R. (1980) Sexual dimorphism, sexual selection and adaptation in phylogenic characters. Evolution 34, 292305.
Litjens, P., Lessinger, A.C. & Azeredo-Espin, A.M.L. (2001). Characterization of screwworm flies Cochliomyia hominivorax and Cochliomyia macellaria by PCR-RFLP of mitochondrial DNA. Medical and Veterinary Entomology 15, 183188.
Ludoski, J., Francuski, L., Vujic, A. & Milankov, V. (2008) The Cheilosia canicularis group (Diptera: Syrphidae): species delimitation and evolutionary relationships based on wing geometric morphometrics. Zootaxa 1825, 4050.
Lyra, M.L., Fresia, P., Gama, S., Cristina, J., Klaczko, L.B. & Azeredo-Espin, A.M.L. (2005) Analysis of Mitochondrial DNA Variability and Genetic Structure in Populations of New World Screwworm Flies (Diptera: Calliphoridae) from Uruguay. Journal of Medical Entomology 42(4), 589595.
Lyra, M.L., Klaczko, L.B. & Azeredo-Espin, A.M.L.Complex pattern of genetic variability in populations of the New World Screwworm fly revealed by mitochondrial DNA markers. Medical and Veterinary Entomology, in press.
Maynard Smith, J. (1978) The Evolution of Sex. 242 pp. Cambridge, UK, Cambridge University Press.
Partridge, L., Barrie, B., Fowler, K. & French, V. (1994) Evolution and development of body size and cell size in Drosophila melanogaster in response to temperature. Evolution 48, 12691276.
Pétavy, G., Moreteau, B., Gibert, P., Morin, J.P. & David, J.R. (2001) Phenotypic plasticity of body size in Drosophila: effects of a daily periodicity of growth temperature in two sibling species. Physiological Entomology 26, 351361.
Pomonis, J.G. (1989) Cuticular hydrocarbons of the screwworm, Cochliomyia hominivorax (Diptera: Calliphoridae). Journal of Chemical Ecology 15, 23012317.
Richardson, R.H., Ellison, J.R. & Averhoff, W.W. (1982) Mating types in Screwworm populations? Reply Science 218, 11431145.
Roehrdanz, R.L. (1989) Intraspecific Genetic Variability in Mitochondrial DNA of the Screwworm Fly (Cochliomyia hominivorax). Biochemical Genetics 27(9–10), 551569.
Rohlf, F.J. (2005a) TPSRegr software for windows version 1.31. Stony Book, NY, Department of Ecology and Evolution, State University of New York. (accessed June 2008).
Rohlf, F.J. (2005b) TPSrelw software for windows version 1.42. Stony Book, NY, Department of Ecology and Evolution, State University of New York. (accessed June 2008).
Rohlf, F.J. (2006) TPSDig software for windows version 2.11. Stony Book, NY, Department of Ecology and Evolution, State University of New York. (accessed June 2008).
Santos, M., Cespedes, W., Balanya, J., Trotta, V., Calboli, F.C.F., Fontdevila, A. & Serra, L. (2006) Temperature-related genetic changes in laboratory populations of Drosophila subobscura: evidence against simple climatic-based explanations for latitudinal clines. American Naturalist 165, 258273.
Shine, R. (1989) Ecological causes for the evolution of sexual dimorphism: a review of the evidence. The Quarterly Review of Biology 64(4), 419461.
Sivinski, J.M. & Dodson, G. (1992) Sexual Dimorphism in Anastrepha suspense (Loew) and other Tephitid fruit flies (Diptera: Tephritidae): Possible rule of developmental rate, fecundity and dispersal. Journal of Insect Behavior 5(4), 491506.
Smith, D.R. & Clevenger, R.R. (1986) Nosocomial nasal myiasis. Archives of Pathology and Laboratory Medicine 110, 439440.
Taylor, D.B., Szalanski, A.L. & Peterson, R.D. II (1996) A polymerase chain reaction – restriction fragment length polymorphism technique for identification of screwworms (Diptera: Calliphoridae). Medical and Veterinary Entomology 10, 6370.
Torres, T.T., Lyra, M.L., Fresia, P. & Azeredo-Espin, A.M.L. (2007) Assessing Genetic Variation in the New World Screwworm, Cochliomyia hominivorax, populations from Uruguay. pp. 183191in Vreysen, M.J.B., Robinson, A.S. & Hendrichs, J. (Eds) Area-Wide Control of Insect Pests: From Research to Field Implementation. Dordrecht, The Netherlands, Springer.
Villemant, C., Simbolotti, G. & Kenis, M. (2007) Discrimination of Eubazus (Hymenoptera, Braconidae) sibling species using geometric morphometrics analysis of wing venation. Systematic Entomology 32, 625634.
Wyss, J.H. (2000) Screwworm Eradication in the Americas. Annals of the New York Academy of Science 791, 241247.
Zelditch, M.L., Swiderski, D.L., Sheets, H.D. & Fink, W.L. (2004) Geometric Morphometrics for Biologists: A Primer. 443 pp. London, UK, Elsevier Academic Press.
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