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Identification of chemosensory gene families in Rhyzopertha dominica (Coleoptera: Bostrichidae)

Published online by Cambridge University Press:  07 May 2015

Mory Mandiana Diakite ,
Juan Wang ,
Suliman Ali  and
Man-Qun Wang
Mory Mandiana Diakite
Affiliation:
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
Juan Wang
Affiliation:
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
Suliman Ali
Affiliation:
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
Man-Qun Wang*
Affiliation:
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
*
1Corresponding author (e-mail: mqwang@mail.hzau.edu.cn).
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Abstract

Chemoreception is a key process for insects. Odorant messages diffuse through the air and are translated into physiological signals by chemosensory receptor neurons in sensilla that are mainly located on insect antennae. We sequenced the antenna transcriptome of Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae), which is a serious pest of stored grains throughout regions with warm climates, and performed transcriptome analysis on R. dominica antennae. We obtained 57 million 90-base pair-long reads that we assembled into 37 877 unigenes with a mean size of 1007 base pairs. Predicted protein sequences were matched with Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) (79.1%), Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae) (1.7%), Megachile rotundata (Fabricius) (Hymenoptera: Megachilidae) (1.3%), Acyrthosiphon pisum Harris (Hemiptera: Aphididae) (1.2%), and other (16.7%) homologues. In chemosensory gene families, we identified transcripts that encoded the following putative genes: 12 odorant-binding proteins (OBPs), four pheromone-binding proteins (PBPs), eight chemosensory proteins (CSPs), five sensory neuron membrane proteins (SNMPs), six odorant receptors, and eight ionotropic receptors. The diversity of the predicted OBPs, PBPs, and CSPs are also discussed. These findings will advance our understanding of olfaction process by this pest.

Type
Physiology, Biochemistry, Development and Genetics
Information
The Canadian Entomologist , Volume 148 , Supplement 1 , February 2016 , pp. 8 - 21
Copyright
© Entomological Society of Canada 2015 

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Footnotes

*

These authors contribute equally to this work.

Subject Editor: Jianghua Sun

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