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SOME FACTORS AFFECTING OVIPOSITION OF TRICHOGRAMMA CALIFORNICUM (HYMENOPTERA: TRICHOGRAMMATIDAE) IN ARTIFICIAL MEDIA1

Published online by Cambridge University Press:  31 May 2012

G. F. Rajendram
Affiliation:
Department of Zoology, University of Sri Lanka, Peradeniya, Sri Lanka

Abstract

Oviposition by Trichogramma californicum Nagaraja and Nagarkatti into physiological saline encapsulated in paraffin droplets is influenced by the concentration of the liquid, population density of the parasite, and the addition of sugars, vitamins, and amino acids. Oviposition occurred within a concentration range of 0.43–1.28% NaCl, but there was no significant difference in the number of eggs deposited. Chances of oviposition generally increased with the increase of population density of the parasite and a minimum density of T. californicum females was required for oviposition. A phenomenon of enhancement, such that oviposition in any one vial is confined to one of the three droplets exposed, is recorded for certain media. However, this phenomenon was removed by addition of RNA, casein hydrolysate, or by use of Grace’s tissue culture medium without insect plasma and Grace’s modified medium with bovine and egg derivatives. Eggs deposited into 2 paraffin droplets containing modified Grace’s medium developed to the second instar larval stage.

Type
Articles
Copyright
Copyright © Entomological Society of Canada 1978

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