Skip to main content
    • Aa
    • Aa

Isolation, mapping and application of a repetitive DNA sequence in wheat (Triticum aestivum) A and B genomes

  • Zeng Zi-Xian (a1), Yang Zu-Jun (a1), Liu Cheng (a1), Hu Li-Jun (a1) and Ren Zheng-Long (a1)...

Simple sequence repeat (SSR) analysis was performed on five Secale species, four Triticum species and a Triticale line Fenzhi-1 using 102 pairs of microsatellite primers. A 387-bp specific DNA fragment FZ387 (GenBank accession no. EF179137) was obtained from the Triticale Fenzhi-1 with primer Xgwm614, without amplification in Secale. NCBI BLAST revealed that this FZ387 sequence had 94% and 95% similarity to part of the Gypsy Ty3-LTR retrotransposon Fatima in Triticum monoccocum (AY485644) and Triticum turgidum (AY494981), respectively. A pair of specific polymerase chain reaction (PCR) primers, FaF and FaR, was designed based on the conserved region of this FZ387 sequence. The amplification of primer pair Xgwm614F and FaR revealed that a specific 350-bp band (designation as A350) was obtained from the species containing A chromosomes. Furthermore, PCR on Langdon Chinese Spring substitution lines was performed, and the results found that this segment was located on both long and short arms of all A chromosomes. However, the amplification of primer pair FaF and Xgwm614R gave rise to a specific DNA band of about 350 bp (designated AB350) from materials containing A and/or B chromosomes. The wild species of wheat and the relatives were amplified using the two pairs of primers, and revealed that only A350 and AB350 were found in Chinese Spring (CS). Sequence comparison and variation of SSR primers binding regions of FZ387 indicated that significant diversity might exist in the internal sequence of this Fatima-like element among triticeae genomes. Meanwhile, both A350 and AB350 can be used as molecular markers for the detection of A and AB genomes.

Corresponding author
*Corresponding authors. E-mail:;
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

ES Dennis , WL Gerlach and WL Peacock (1980) Identical polypyrimidine-polypurine satellite DNA in wheat and barley. Heredity 44: 349366.

RB Flavell and DB Smith (1976) Nucleotide sequence organization in the wheat genome. Heredity 37: 231252.

R Kalendar , T Grob , M Regina , A Suoniemi and A Schulman (1999) IRAP and REMAP: two new retrotransposon-based DNA finger-printing techniques. Theoretical Applied Genetics 98: 704711.

C Liu , ZJ Yang , J Feng , JP Zhou , SH Chi and ZL Ren (2006) Development of Dasypyrum genome specific marker by using wheat microsatellites. Hereditas (Beijing) 28: 15731579.

C Liu , ZJ Yang , C Liu , GR Li and ZL Ren (2007) Analysis of St-chromosome-containing triticeae polyploids using specific molecular markers. Hereditas (Beijing) 29: 12711279.

C Liu , ZJ Yang , GR Li , (2008) Isolation of a new repetitive DNA sequence from Secale africanum enables targeting of Secale chromatin in wheat background. Euphytica 159: 249258.

CJ Liu , MD Atkinson , CN Chinoy , KM Devos and MD Gale (1992) Nonhomoeologous translocations between group 4, 5 and rye. Theoretical Applied Genetics 83: 305312.

AL Rayburn and BS Gill (1986) Isolation of a D-genome specific repeated DNA sequence from Aegilops squarrosa. Plant Molecular Biology Report 4: 104109.

AV Vershinin and THN Ellis (1999) Heterogeneity of the internal structure of PDR1, a family of Ty1/copia-like retrotransposons in pea. Molecular and General Genetics 262: 703713.

R Waugh , K McLean , AJ Flavell , (1997) Genetic distribution of BARE-1-like retrotransposable elements in the barley genome revealed by sequence-specific amplification polymorphisms (S-SAP). Molecular and General Genetics 253: 687–684.

ZJ Yang , GR Li , J Feng , HR Jiang and ZL Ren (2005) Molecular cytogenetic characterization and disease resistance observation of wheat–Dasypyrum breviaristatum partial amphiploid and its derivatives. Hereditas 142: 8085.

ZJ Yang , C Liu , J Feng , (2006) Studies on genome relationship and species-specific PCR marker for Dasypyrum breviaristatum in Triticeae. Hereditas 143: 4754.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Chinese Journal of Agricultural Biotechnology
  • ISSN: 1479-2362
  • EISSN: 1479-2370
  • URL: /core/journals/chinese-journal-of-agricultural-biotechnology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *



Full text views

Total number of HTML views: 0
Total number of PDF views: 1 *
Loading metrics...

Abstract views

Total abstract views: 53 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 29th May 2017. This data will be updated every 24 hours.