Hostname: page-component-5d84bcc8dc-vfbgv Total loading time: 0 Render date: 2026-09-13T13:43:38.236Z Has data issue: false hasContentIssue false
  • English
  • Français

Electro-fluorescence studies of the binding of fluorescent dyes to sepiolite

Published online by Cambridge University Press:  09 July 2018

S. A. Windsor ,
N. J. Harrison  and
M. H. Tinker
S. A. Windsor
Affiliation:
Optics group, J. J. Thomson Physical Laboratory, Whiteknights, Reading, RG6 6AF, UK
N. J. Harrison*
Affiliation:
Optics group, J. J. Thomson Physical Laboratory, Whiteknights, Reading, RG6 6AF, UK
M. H. Tinker
Affiliation:
Optics group, J. J. Thomson Physical Laboratory, Whiteknights, Reading, RG6 6AF, UK
*
*Current address: The National Physical Laboratory, Teddington, Middlesex, TW11 0LW, UK
Get access Rights & Permissions [Opens in a new window]

Abstract

Aqueous suspensions of sepiolite, tagged with fluorescent dyes, have been studied using electro-fluorescence polarization spectroscopy. The binding modes of some 37 fluorescent dyes and optical brightening agents to the rod-like sepiolite particles have been determined. Many of the dyes are found to bind with a degree of order to the clay particle's major axis. The binding geometries of the cationic dye molecules tested were found to be dependent upon molecular size. This supports the view that these cationic dye molecules are constrained within the channels which are characteristic of the mineral sepiolite. Results for uncharged and anionic dye molecules are also presented; no dependence of binding geometry upon molecular size was found. The anionic molecules are most likely to associate with the exterior cationic magnesium surface. The results indicate that some of the anionic dyes are too large to fit in the channels. Some of the uncharged molecules adopt a number of orientations upon binding which gives rise to an average geometry being observed for these dyes.

Information

Type
Research Article
Information
Clay Minerals , Volume 31 , Issue 1 , March 1996 , pp. 81 - 94
Copyright
Copyright © The Mineralogical Society of Great Britain and Ireland 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Appleyard, J. & Crabbe, M.J.C. (1991) Desktop Molecular Modeller, version 2.0. Oxford University Press, Oxford.Google Scholar
Benoit, H. (1951) Contribution a l'etude de l'effect Kerr présenté par les solutions diluées de macromotécules rigides. Ann. Phys. - Paris, 6, 561–609.CrossRefGoogle Scholar
Brauner, K. & Presslnger, A. (1956) Struktur und entstehung des Sepioliths. Mineral Pereog. Mitt. 4, 120–140.Google Scholar
Fredericq, E. & Houssmr, C. (1973) Electric Dichroism and Electric Birefringence. Clarendon Press, Oxford.Google Scholar
Giannelis, E.P., Rightor, E.G. & Pfnnavaia, T.J. (1988) Reactions of metal-cluster carbonyls in pillared clay galleries–surface coordination chemistry and Fischer-Tropsch catalysis. J. Am. Chem. Soc. 110, 3880–3885.Google Scholar
Hogan, M., Dattagupta, N. & Croti-Iers, D.M. (1978) Transient electric dichroism of rod-like DNA molecules. Proc. Nat. Acad. Sci. USA 75, 195–199.Google Scholar
Houssier, C., Hardy, B. & Fredericq, E. (1974) Interaction of Ethidium Bromide with DNA. Optical and electrooptical study. Biopolymers, 13, 1141–1160.Google Scholar
Jenmngs, B.R. (1989) Electrofluorescence: an experimental appraisal. Pp. 93-–102 in: Dynamic Behaviour qf Macromolecules, Colloids, Liquid Crystals and Biological Systems (Watanabe, H., editor). Hirokawa, Tokyo.Google Scholar
Jennings, B.R. & Ridler, P.J. (1989) Electro-optic fluorescence for optical shutters. J. Phys. D Appl. Phys. 22, 337–342.Google Scholar
Middleton, G.W. & Jennings, B.R. (1991) Electrofluorescence of dye-tagged sepiolite. Clay Miner. 26, 1–9.CrossRefGoogle Scholar
Nagy, B. & Bradley, W.F. (1955) The structural scheme of sepiolite. Am. Miner. 40, 885–892.Google Scholar
Nijs, H., Fripiat, J.J. & Van Damme, H.J. (1983) Visiblelight-induced cleavage of water in colloidal clay suspensions–a new example of oscillatory reaction at interfaces. J. Phys. Chem. 87, 1279–1282.Google Scholar
Perrin, F. (1934) Mouvement Brownien d'un ellipsoide (I). Dispersion didlectrique pour des molécules ellpsoidales. J. Phys. Rad. 5, 487–511.Google Scholar
Ridler, P.J. & Jennings, B.R. (1979) Electrically induced fluorescence changes from solutions of dye-tagged polyribonucleotides. Pp. 99–107 in: Electro-optics and Dielectrics of Macromolecules and Colloids (Jennings, B.R., editor). Plenum, New York.Google Scholar
Ridler, P.J. & Jennings, B.R. (1980) Transient fluorescence studies on sepiolite suspensions. Clay Miner. 15, 121–133.CrossRefGoogle Scholar
Rldler, P.J., Jennings, B.R., Osborne, M., & Brookes, P. (1986) Electrofluorescence study of polycyclic hydrocarbon diol-epoxide binding to DNA. Proc R. Soc., Ser. B 227, 441–454.Google Scholar
Villemure, G., Detellmr, C. & Szabo, A.G. (1991) Fluorescence of methylviologen intercalated into Montmorillonite and Hectorite aqueous suspensions. Langmuir 7, 1215–1221.Google Scholar
Windsor, S.A. & Tinker, M.H. (1995) The binding of biologically important molecules to DNA, probed using electro-fluorescence polarization spectroscopy. J. Biophys. Chem. (in press).Google Scholar