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Inflammatory responses to three modes of intense exercise in Standardbred mares – a pilot study

  • E D Lamprecht (a1), C A Bagnell (a1) and C A Williams (a1)
Abstract
Abstract

The objective of this study was to compare exercise-induced inflammatory responses in horses undergoing three different treadmill exercise tests. Mares completed a graded exercise test (GXT), an interval exercise test (IET) and a repeated sprint exercise test (RSET). Blood and synovial fluid samples were taken 24 h before (PRE) exercise, 20–30 min, 2 and 24 h following exercise (REC). Blood was analysed for total protein, haematocrit and total nitrite concentration. Pro-inflammatory and anti-inflammatory cytokine transcripts were measured in whole blood using quantitative real-time polymerase chain reaction. Synovial fluid was analysed for total nitrite. Mares spent more time at greater than 90% of their maximum heart rate during the RSET than they did for either the GXT or IET. There was an effect of exercise test (P < 0.0001) and exercise test by sample interaction (P = 0.010) for plasma total nitrite. Pro-inflammatory cytokine transcripts and plasma total protein were elevated (P < 0.05) after exercise and there was a higher (P < 0.0001) nitrite concentration in tibiotarsal joints compared with radiocarpal joints. As hypothesised, the higher-intensity exercise test (RSET) resulted in greater nitric oxide responses as well as markers of exercise intensity compared with less intense exercise tests (IET, GXT).

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*Corresponding author: lamprecht@aesop.rutgers.edu
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1 A Higgins and P Lees (1984). The acute inflammatory process, arachidonic acid metabolism and the mode of action of anti-inflammatory drugs – a review. Equine Veterinary Journal 16: 163175.

2 J Han and R Ulevitch (2005). Limiting inflammatory responses during activation of innate immunity – a review. Nature Immunology 6: 11981205.

3 J Cannon and M Kluger (1983). Endogenous pyrogen activity in human plasma after exercise. Science 220: 617619.

4 A Moldoveanu , R Shephard and P Shek (2001). The cytokine response to physical activity and training (Review). Sports Medicine 31: 115144.

5 I Brenner , V Natale , P Vasiliou , A Moldoveanu , P Shek and R Shephard (1999). Impact of three different types of exercise on components of the inflammatory response. European Journal of Applied Physiology 80: 452460.

9 A Sureda , P Tauler , A Aguilo , E Fuentespina , A Cordova , J Tur and A Pons (2006). Blood cell NO synthesis in response to exercise. Nitric Oxide 15: 512.

10 J Bird , T Wells , D Platt and M Bayliss (1997). IL-1 beta induces the degradation of equine articular cartilage by a mechanism that is not mediated by nitric oxide. Biochemical and Biophysical Research Communications 238: 8185.

11 D Kim , H Taylor , R Moore , D Paulsen and D Cho (2003). Articular chondrocyte apoptosis in equine osteoarthritis. The Veterinary Journal 166: 5257.

12 G Murrell , D Jang and R Williams (1995). Nitric oxide activates metalloprotease enzymes in articular cartilage. Biochemical and Biophysical Research Communications 206: 1521.

13 A Farrell , D Blake , R Parlmer and S Moncada (1992). Increased concentrations of nitrite in synovial fluid and serum samples suggest increased nitric oxide synthesis in rheumatic diseases. Annals of the Rheumatic Diseases 51: 12191222.

15 A Dimock , P Siciliano and C McIlwraith (2000). Evidence supporting an increased presence of reactive oxygen species in the diseased equine joint. Equine Veterinary Journal 32: 439443.

16 R Van den Boom , C van de lest , S Bull , P Brama , P van Weeren and A Barneveld (2005). Influence of repeated arthrocentesis and exercise on synovial fluid concentrations of nitric oxide, prostaglandin E2 and glycosaminoglycans in healthy equine joints. Equine Veterinary Journal 37: 250256.

17 D Auer , J Ng , J Hrdlicka and A Seawright (1989). The elimination of injected superoxide dismutase from synovial fluid of the horse. Australian Veterinary Journal 66: 117119.

18 D Henneke , G Potter , J Kreider and B Yeates (1983). Relationship between condition score, physical measurement, and body fat percentage in mares. Equine Veterinary Journal 15: 371372.

19 R Westervelt , H Stouffer , H Hintz and H Schryver (1976). Estimating fatness in horses and ponies. Journal of Animal Science 43: 781785.

22 P Graham-Thiers , D Kronfeld , K Kline , D Sklan and P Harris (2003). Dietary protein and fat effects on protein status in Arabian horses during interval training and repeated sprints. Journal of Equine Veterinary Science 23: 554559.

23 J Wilson , D Kronfeld , L Gay , J Williams , T Wilson and M Lindinger (1998). Sarcoplasmic reticulum responses to repeated sprints are affected by conditioning of horses. Journal of Animal Science 76: 30653071.

24 M Vick , A Adams , B Murphy , D Sessions , D Horohov , R Cook , B Shelton and B Fitzgerald (2007). Relationships among inflammatory cytokines, obesity, and insulin sensitivity in the horse. Journal of Animal Science 85: 11441155.

25 D Ainsworth , J Appleton , S Eicker , R Luce , M Flaminio and F Antczak (2003). The effect of strenuous exercise on mRNA concentrations of interleukin-12, interferon-gamma and interleukin-4 in equine pulmonary and peripheral blood mononuclear cells. Veterinary. Immunology and Immunopathology 91: 6171.

26 P Colahan , C Kollias-Baker , C Leutenegger and J Jones (2002). Does training affect mRNA transcription for cytokine production in circulating leucocytes? Equine Veterinary Journal Supplement 34: 154158.

27 K Ostrowski , T Rohde , S Asp , P Schjerling and B Pedersen (1999). Pro- and anti-inflammatory cytokine balance in strenuous exercise in humans. Journal of Physiology 515: 287291.

28 A Cross (1996). Novel approaches to the treatment of sepsis: lessons learned and future directions. Shock 6: 7174.

29 S Rhind , P Shek , S Shinkai and R Shephard (1996). Effects of moderate endurance exercise and training on in vitro lymphocyte proliferation, interleukin-2, production, and IL-2 receptor expression. European Journal of Applied Physiology 74: 348360.

30 J Peake , K Suzuki , M Hordern , G Wilson , K Nosaka and J Coombes (2005). Plasma cytokine changes in relation to exercise intensity and muscle damage. European Journal of Applied Physiology 95: 514521.

31 M Flynn , B McFarlin and M Markofski (2007). The anti-inflammatory actions of exercise training. American Journal of Lifestyle Medicine 1: 220235.

32 N Moyna , G Acker , J Fulton , K Weber , F Goss , R Roberson , D Tollerud and B Rabin (1996). Lymphocyte function and cytokine production during incremental exercise in active and sedentary males and females. International Journal of Sports Medicine 17: 585591.

33 V Pavlov and K Tracey (2004). Review – neural regulators of innate immune responses and inflammation. Cellular and Molecular Life Sciences 61: 23222331.

34 G Gannon , S Rhind , M Suzuki , P Shek and R Shaphard (1997). Circulating levels of peripheral blood leucocytes and cytokines following competitive cycling. Canadian Journal of Applied Physiology 22: 133147.

35 A Niess , I Passek Lorenz , H Dickhuth , H Northoff and E Fehrenback (1999). Expression of the antioxidant stress protein heme oxygenase-I (HO-I) I human leukocytes. Acute and adaptational response to endurance exercise. Journal of Free Radicals and Biology in Medicine 26: 184192.

36 H Bruunsgaard , H Galbo , J Halkjaer-Kristensen , T Johansen , D MacLean and B Pedersen (1997). Exercise-induced increase in serum interleukin-6 in humans is related to muscle damage. Journal of Physiology 499: 833841.

38 P Mucci , F Durand , B Lebel , J Bousquet and C Prefaut (2000). Interleukins 1-beta, -8, and histamine increases in highly trained, exercising athletes. Medicine and Science in Sports and Exercise 32: 10941100.

40 K Suzuki , S Nakaji , M Yamada , Q Liu , S Kurakake , N Okamura , T Kumae , T Umeda and K Sugawara (2003). Impact of a competitive marathon race on systemic cytokine and neutrophil responses. Medicine and Science in Sports and Exercise 35: 348355.

41 H Sprenger , C Jacobs , M Nain , A Gressner , H Prinz , W Wesemann and D Gemsa (1992). Enhanced release of cytokines, interleukin-2 receptors, and neopterin after long-distance running. Clinical Immunology and Immunopathology 63: 188195.

42 A Petersen and B Pedersen (2005). The anti-inflammatory effect of exercise. Journal of Applied Physiology 98: 11541162.

43 K Ostrowski , C Hermann , A Bangash , P Schjerling , J Nielsen and B Pedersen (1998). A trauma-like elevation of plasma cytokines in humans in response to treadmill running. Journal of Physiology 513: 889894.

44 B Pedersen (2000). Special feature for the Olympics: effects of exercise on the immune system: exercise and cytokines. Immunology and Cell Biology 78: 532535.

45 D MacIntyre , S Sorichter , J Mair , A Berg and D McKenzie (2001). Markers of inflammation and myofibrillar proteins following eccentric exercise in humans. European Journal of Applied Physiology 84: 180186.

46 P Haahr , B Pedersen , A Fomsgaard , N Tvede , M Diamant , K Klarlund , J Halkjaer-Kristensen and K Bendtzen (1991). Effect of physical exercise on in vitro production of interleukin 1, interleukin 6, tumour necrosis factor-alpha, interleukin 2 and interferon-gamma. International Journal of Sports Medicine 12: 223227.

47 C Kindig , L Gallatin , H Erickson , M Fedde and D Poole (2000). Cardiorespiratory impact of the nitric oxide synthase inhibitor l-NAME in the exercising horse. Respiratory Physiology 120: 151156.

50 H Cai and D Harrison (2000). Endothelial dysfunction in cardiovascular diseases: the role of oxidant stress. Circulation Research 87: 841844.

51 P Tauler , A Auuilo , N Cases , A Sureda , F Gimenez , G Villa , A Cordova and A Biescas (2002). Acute phase immune response to exercise coexists with decreased neutrophil antioxidant enzyme defenses. Free Radical Research 36: 11011107.

54 O Jung , S Marklund , H Geiger , T Pedrazzini , R Busse and R Brandes (2003). Extracellular superoxide dismutase is a major determinant of nitric oxide bioavailability. Circulation Research 93: 622629.

55 L Jonsson , D Rees , T Edlund and S Marklund (2002). Nitric oxide and blood pressure in mice lacking extracellular-superoxide dismutase. Free Radical Research 36: 755758.

56 R Van den boom , P Brama , G Kiers , J DeGroot , A Barneveld and R van Weeren (2004). The influence of repeated arthrocentesis and exercise on matrix metalloproteinase and tumour necrosis factor alpha activities in normal equine joints. Equine Veterinary Journal 36: 155159.

58 D Dutto , D Hoyt , E Cogger and S Wickler (2004). Ground reaction forces in horses trotting up an incline and on the level over a range of speeds. Journal of Experimental Biology 207: 35073514.

59 J Fenton , K Chlebek-Brown , T Peters , J Caron and M Orth (2000). Glucosamine HCl reduces equine articular cartilage degradation in explant culture. Osteoarthritis and Cartilage 8: 258265.

61 M Stefanovic-Racic , K Meyers , C Meschter , J Coffey , R Hoffman and C Evans (1994). N-monomethyl arginine, an inhibitor of nitric oxide synthase, suppresses the development of adjuvant arthritis in rats. Arthritis and Rheumatism 37: 10621069.

63 M Wiseman , F Henson , D Lee and D Bader (2003). Dynamic compressive strain inhibits nitric oxide synthesis by equine chondrocytes isolated from different areas of the cartilage surface. Equine Veterinary Journal 35: 451456.

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Comparative Exercise Physiology
  • ISSN: 1755-2540
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