Hostname: page-component-848d4c4894-2pzkn Total loading time: 0 Render date: 2024-04-30T14:57:06.068Z Has data issue: false hasContentIssue false
  • English
  • Français

Inheritance of GFP-Bt transgenes from Brassica napusin backcrosses with three wild B. rapa accessions

Published online by Cambridge University Press:  15 March 2004

Bin Zhu ,
John R. Lawrence ,
Suzanne I. Warwick ,
Peter Mason ,
Lorraine Braun ,
Matthew D. Halfhill  and
C. Neal Stewart Jr.
Bin Zhu
Affiliation:
 National Water Research Institute, Environment Canada, 11 Innovation Blvd, Saskatoon, Saskatchewan, S7N 3H5, Canada
John R. Lawrence
Affiliation:
 National Water Research Institute, Environment Canada, 11 Innovation Blvd, Saskatoon, Saskatchewan, S7N 3H5, Canada
Suzanne I. Warwick
Affiliation:
 Agriculture and Agri-Food Canada-ECORC, 960 Carling Ave, Ottawa, Ontario, K1A 0C6, Canada
Peter Mason
Affiliation:
 Agriculture and Agri-Food Canada-ECORC, 960 Carling Ave, Ottawa, Ontario, K1A 0C6, Canada
Lorraine Braun
Affiliation:
 Agriculture and Agri-Food Canada-Saskatoon Research Centre, 107 Science Place, Saskatoon, Saskatchewan, S7N 0X2, Canada
Matthew D. Halfhill
Affiliation:
 Dept. of Plant Sciences, 2431 Centre Drive, Ellington Plant Sciences, University of Tennessee, Knoxville, Tennessee 37996-4561, USA
C. Neal Stewart Jr.
Affiliation:
 Dept. of Plant Sciences, 2431 Centre Drive, Ellington Plant Sciences, University of Tennessee, Knoxville, Tennessee 37996-4561, USA

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Transgenes from transgenic oilseed rape, Brassica napus (AACC genome), can introgress into populations of wild B. rapa (AA genome), but little is known about the long-term persistence of transgenes from different transformation events. For example, transgenes that are located on the crop’s C chromosomes may be lost during the process of introgression. We investigated the genetic behavior of transgenes in backcross generations of wild B. rapa after nine GFP (green fluorescent protein)-Bt (Bacillus thuringiensis) B. napus lines, named GT lines, were hybridized with three wild B. rapa accessions, respectively. Each backcross generation involved crosses between hemizygous GT plants and non-GT B. rapa pollen recipients. In some cases, sample sizes were too small to allow the detection of major deviations from Mendelian segregation ratios, but the segregation of GT:non-GT was consistent with an expected ratio of 1:1 in all crosses in the BC1 generation. Starting with the BC2 generation, significantly different genetic behavior of the transgenes was observed among the nine GT B. napus lines. In some lines, the segregation of GT:non-GT showed a ratio of 1:1 in the BC2, BC3, and BC4 generations. However, in other GT B. napus lines the segregation ratio of GT:non-GT significantly deviated from 1:1 in the BC2 and BC3 generations, which had fewer transgenic progeny than expected, but not in the BC4 generation. Most importantly, in two GT B. napus lines the segregation of GT:non-GT did not fit into a ratio of 1:1 in the BC2, BC3 or BC4 generations due to a deficiency of transgenic progeny. For these lines, a strong reduction of transgene introgression was observed in all three B. rapa accessions. These findings imply that the genomic location of transgenes in B. napus may affect the long-term persistence of transgenes in B. rapa after hybridization has occurred.

Keywords

transgeneoilseed rapeB. campestrisinterspecific hybridizationbackcrossintrogressiongreen fluorescent protein (GFP)Bacillus thuringiensis (Bt)
Type
Research Article
Information
Environmental Biosafety Research , Volume 3 , Issue 1 , January 2004 , pp. 45 - 54
Copyright
© ISBR, EDP Sciences, 2004

References

Attia, T, Röbbelen, G (1986) Meiotic pairing in haploids and amphidiploids of spontaneous versus synthetic origin in rape, Brassica napus L. Can. J. Genet. Cytol. 28: 330–334 CrossRef
Attia, T, Busso, C, Röbbelen, G (1987) Digenomic triploids for an assessment of chromosome relationships in the cultivated diploid Brassica species. Genome 29: 326–330 CrossRef
Chen BY, Cheng BF, Jørgensen RB, Heneen WK (1997) Production and cytogenetics of Brassica campestris-alboglabra chromosome addition lines. Theor. Appl. Genet. 94: 633–640
Halfhill, MD, Richards, HA, Mabon, SA, Stewart, CN Jr. (2001) Expression of GFP and Bt transgenes in Brassica napus and hybridization with Brassica rapa. Theor. Appl. Genet. 103: 659–667 CrossRef
Harper, BK, Mabon, SA, Leffel, SM, Halfhill, MD, Richards, HA, Moyer, KA, Stewart, CN Jr. (1999) Green fluorescent protein as a marker for expression of a second gene in transgenic plants. Nat. Biotechnol. 17: 1125–1129
Heneen, WK, Chen, BY, Cheng, BF, Jonsson, A, Simonsen, V, Jørgensen, RB, Davik, J (1995) Characterization of the A and C genomes of Brassica campestris and B. alboglabra. Hereditas 123: 251–267 CrossRef
Heneen, WK, Jørgensen, RB (2001) Cytology, RAPD, and seed color of progeny plants from Brassica rapa-alboglabra aneuploids and development of monosomic addition lines. Genome 44: 1007–1021 CrossRef
Hoffman, CA (1990) Ecological risks of genetic engineering of crop plants. BioScience 40: 434–437 CrossRef
Holm LG, Doll J, Holm E, Pancho J, Herberger J (1997) Brassica campestris L. In World Weeds: Natural Histories and Distribution. John Wiley & Sons, Inc., New York, pp 117–124
Jørgensen RB, Andersen B (1994) Spontaneous hybridization between oilseed rape (Brassica napus) and weedy B. campestris (Brassicaceae): a risk of growing genetically modified oilseed rape. Am. J. Bot. 81: 1620–1626
Jørgensen, RB, Andersen, B, Landbo, L, Mikkelsen, TR (1996a) Spontaneous hybridization between oilseed rape (Brassica napus) and weedy relatives. Acta Hort. 407: 193–200 CrossRef
Jørgensen, RB, Hauser, T, Mikkelsen, TR, Østergård H (1996b) Transfer of engineered genes from crop to wild plants. Trends Plant Sci. 1: 356–358 CrossRef
Lu CM, Kato M, Kakihara F (2002) Destiny of a transgene escape from Brassica napus into Brassica rapa. Theor. Appl. Genet. 105: 78–84
McGrath, JM, Quiros, CF (1990) Generation of alien chromosome addition lines from synthetic Brassica napus: morphology, cytology, fertility, and chromosome transmission. Genome 33: 374–383 CrossRef
McGrath, JM, Quiros, CF (1991) Inheritance of isozyme and RFLP markers in Brassica campestris and comparison with B. oleracea. Theor. Appl. Genet. 82: 668–673 CrossRef
Metz, PLJ, Jacobsen, E, Nap, JP, Pereira, A, Stiekema, WJ (1997) The impact on biosafety of the phosphinothricin-tolerance transgene in inter-specific B. rapa × B. napus hybrids and their successive backcrosses. Theor. Appl. Genet. 95: 442–450 CrossRef
Mikkelsen, TR, Andersen, B, Jørgensen, RB (1996a) The risk of crop transgene spread. Nature 380: 31 CrossRef
Mikkelsen, TR, Jensen, J, Jørgensen, RB (1996b) Inheritance of oilseed rape (Brassica napus) RAPD markers in a backcross progeny with Brassica campestris. Theor. Appl. Genet. 92: 492–497 CrossRef
Nozaki T, Mishiba K, Mii M, Koba T (2000) Construction of synteny groups of Brassica alboglabra by RAPD markers and detection of chromosome aberrations and distorted transmission under the genetic background of B. campestris. Theor. Appl. Genet. 101: 538–546
Raybould, AF, Gray, AJ (1993) Genetically modified crops and hybridization with wild relatives: a UK perspective. J. Appl. Ecol. 30: 199–219 CrossRef
Scheffler, JA, Dale, PJ (1994) Opportunities for gene transfer from transgenic oilseed rape (Brassica napus) to related species. Transgenic Res. 3: 263–278 CrossRef
Scott, SE, Wilkinson, MJ (1998) Transgene risk is low. Nature 393: 320 CrossRef
Snow, AA (2002) Transgenic crops – why gene flow matters. Nat. Biotechnol. 20: 542 CrossRef
Snow AA, Andersen B, Jørgensen RB (1999) Costs of transgenic herbicide resistance introgressed from Brassica napus into weedy B. rapa. Mol. Ecol. 8: 605–615
Somers DJ, Friesen KRD, Rakow G (1998) Identification of molecular markers associated with linoleic acid desaturation in Brassica napus. Theor. Appl. Genet. 96: 897–903
Stewart, CN Jr., Adang, MJ, All, JN, Boerma, HR, Cardineau, G, Tucker, D, Parrott, WA (1996) Genetic transformation, recovery, and characterization of fertile soybean transgenic for a synthetic Bacillus thuringiensis cryIAc gene. Plant Physiol. 112: 121–129 CrossRef
Tomiuk, J, Hauser, TP, Bagger-Jørgensen R (2000) A- or C-chromosomes, does it matter for the transfer of transgenes from Brassica napus. Theor. Appl. Genet. 100: 750–754 CrossRef
U N (1935) Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Jpn. J. Bot. 7: 389–452
Warwick, SI, Simard, MJ, Légère, A, Beckie, HJ, Braun, L, Zhu, B, Mason, P, Séguin Swartz, G, Stewart, CN Jr (2003) Hybridization between transgenic Brassica napus L. and its wild relatives: B. rapa L., Raphanus raphanistrum L., Sinapis arvensis L., and Erucastrum gallicum (Willd.) O. E. Schulz. Theor. Appl. Genet. 107: 528–539 CrossRef
Wilkinson, MJ, Elliott, LJ, Allainguillaume, J, Shaw, MW, Norris, C, Welters, R, Alexander, M, Sweet, J, Mason, DC (2003) Hybridization between Brassica napus and B. rapa on a national scale in the United Kingdom. Science 302: 457–459 CrossRef