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An extended model for transfer of micro-organisms via the hands: differences between organisms and the effect of alcohol disinfection

Published online by Cambridge University Press:  19 October 2009

C. A. Mackintosh  and
P. N. Hoffman
C. A. Mackintosh
Affiliation:
Division of Hospital Infection, Central Public Health Laboratory, Colindale, London NW9 5HT
P. N. Hoffman
Affiliation:
Division of Hospital Infection, Central Public Health Laboratory, Colindale, London NW9 5HT
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A model for contact transfer of micro-organisms by hand has been extended to include representatives of bacterial species responsible for a majority of hospitalacquired infections. The ability of the organisms to transfer from contaminated fabrics to hands and from hands to sterile fabrics was measured, as was their ability to survive on the skin of the hands. There were differences between the species. Staphylococcus saprophyticus transferred well to the hand but not as well from hand to fabric as the other species; it survived well on skin. Pseudomonas aeruginosa, Klebsiella aerogenes and Serratia marcescens transferred moderately well overall and also survived on the skin. These results were in contrast to those obtained with a strain of Escherichia coli and one of Streptococcus pyogenes.

The contact transfer model was used to investigate the use of small volumes of alcohol in preventing transfer via the hands. An alcohol handrub of either 0·3 ml 80% ethanol or 0·3 ml 70% isopropanol gave reductions in transfer slightly less than that of a soap and water wash. Raising the volume, and consequently the contact time, to 0·5 ml 70% isopropanol gave a 14000-fold reduction in transfer, statistically indistinguishable from that of a thorough soap and water wash (9800-fold reduction).

Type
Research Article
Information
Journal of Hygiene , Volume 92 , Issue 3 , June 1984 , pp. 345 - 355
Copyright
Copyright © Cambridge University Press 1984

References

Albert, R. K. & Condie, F. (1981). Hand washing patterns in medical intensive care units. New England Journal of Medicine 304, 1465–1466.CrossRefGoogle ScholarPubMed
Aly, R. & Maibach, H. I. (1980). A comparison of the antimicrobial effect of 0·5% chlorhexidine and 70% isopropyl alcohol on hands contaminated with Serratia marcescens. Clinical and Experimental Dermatology 5, 197–201.Google Scholar
Ayliffe, G. A. J., Babb, J. R. & Quoraishi, A. J. (1978). A test for ‘hygienic’ hand disinfection. Journal of Clinical Pathology 31, 923–928.Google Scholar
Dineen, P. (1978). Hand-washing degerming. A comparison of povidone-iodine and chlorhexidine. Clinical Pharmacology & Therapeutics 23, 62–67.CrossRefGoogle ScholarPubMed
Hart, C. A., Gibson, M. F. & Buckles, A. M. (1981). Variation in skin and environmental survival of gentamicin-resistant enterobacteria. Journal of Hygiene 87, 277–285.Google Scholar
Knittle, M. A., Eitzman, D. V. & Baer, H. (1975). Role of hand contamination of personnel in the epidemiology of Gram-negative nosocomial infections. Journal of Paediatrics 86, 433–437.Google Scholar
Marples, R. R. & Towers, A. G. (1979). A laboratory model for the investigation of contact transfer of micro-organisms. Journal of Hygiene 82, 237–248.Google Scholar
Meers, P. D. & Yeo, G. A. (1978). Shedding of bacteria and skin squames after handwashing. Journal of Hygiene 81, 99–105.CrossRefGoogle ScholarPubMed
Ojajärvi, J., Makela, P. & Rantasalo, I. (1977). Failure of hand disinfection with frequent hand washing: a need for prolonged field studies. Journal of Hygiene 79, 107–119.Google Scholar
Rotter, M., Koller, W. & Kundi, M. (1977). Usability of three alcohols for a standard disinfection method to be employed for the evaluation of procedures for the hygienic disinfection of hands. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abt. 1, Orig. B) 164, 428–438.Google ScholarPubMed
Rotter, M., Koller, W. & Wewalka, G. (1980). Povidone-iodine and chlorhexidine gluconate containing detergents for disinfection of hands. Journal of Hospital Infection 1, 149–158.CrossRefGoogle ScholarPubMed
Saltzman, T. C., Clark, J. C. & Klemm, L. M. (1967). Hand contamination of personnel as a mechanism of cross-infection in nosocomial infections with antibiotic-resistant Escherichia coli and Klebsiella–Aerobacter. Antimicrobial Agent and Chemotherapy 6, 97–100.Google Scholar
Steere, A. C. & Mallison, G. F. (1975). Handwashing practices for the prevention of nosocomial infection. Annals of Internal Medicine 83, 683–690.Google Scholar
Stringer, M. F. & Marples, R. R. (1976). Ultrasonic methods for sampling human skin micro-organisms. British Journal of Dermatology 94, 551–555.Google Scholar
Taylor, L. J. (1978 a). An evaluation of handwashing techniques. 1. Nursing Times 74, 54–55.Google Scholar
Taylor, L. J. (1978 b). An evaluation of handwashing techniques. 2. Nursing Times 74, 108–110.Google ScholarPubMed
Williamson, P. & Kligman, A. M. (1965). A new method for the quantitative investigation of cutaneous bacteria. Journal of Investigative Dermatology 45, 498–503.Google Scholar