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Antigenic relationship of West Nile strains by titre ratios calculated from cross-neutralization test results

  • N. K. Blackburn (a1), D. L. Thompson (a1) and P. G. Jupp (a1)

The antigenic relationship of ten South African West Nile isolates, the South African prototype virus H442, the Egyptian strain EG101 and the Indian strain G2266 were compared using titre ratios. The titre ratios or ‘R’ values were calculated from heterologous and homologous neutralization titres and expressed as a percentage.

Substantial antigenic differences were demonstrated between the South African prototype strain and the majority of the recently obtained South African isolates, seven of which were fairly closely related and possibly form a distinct antigenic sub-set. The recent isolates also differed from the Egyptian and Indian West Nile isolates. The heterologous results between the South African West Nile strains and the Indian strain G2266 suggest that prior infection with an Indian West Nile virus would give poor protection against the South African viruses, whereas the reverse would not be so.

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Blackburn N. K. & Swanepoel R. (1980). An investigation of flavivirus infections of cattle in Zimbabwe Rhodesia with particular reference to Wesselsbron virus. Journal of Hygiene 85, 133.
Hammam H. M., Clarke D. H. & Price W. H. (1965). Antigenic variations of West Nile virus in relation to geography. American Journal of Epidemiology 82, 4055.
Jordan W. S. & Gaylin S. G. (1953). Tho antigenic variation of influenza B viruses. Demonstration of an antigenic spectrum by use of titre ratios. Journal of Immunology 70, 303309.
Jupp P. G., Blackburn N. K., Thompson D. L. & Meenehan G. M. (1980). Sindbis and West Nile virus infections in the Witwatersrand-Pretoria region. South African Medical Journal 70, 218220.
Kärber G. (1931). Beitrag zur kollektiven Behandlung pharmakologischcr Reihensverouche. Archiv für Experimented Palhologie und Pharmakologie 162, 480483.
Mah Lee Ng & Westaway E. G. (1983). Phenotypic changes in the Flavivirus Kunjin after a single cycle of growth in an Aedes albopictus cell line. Journal of General Virology 64, 17151723.
Nir Y., Goldwasser R., Lasowski Y. & Margalit J. (1998). Isolation of West Nile virus strains from mosquitoes in Tsrael. American Journal of Epidemiology 87, 400501.
Odelala M. A. & Fabiyi A. (1976). Antigenic relationships among Nigerian strains of West Nile virus by complement fixation and agar gel precipitation techniques. Transactions of the Royal Society of Tropical Medicine and Hygiene 70, 138140.
Rweyemamu M. M., Booth J. C., Parry N. & Pay T. W. F. (1977). Neutralization kinetics studies with type SAT 2 foot-and-mouth disease virus strains. II. Antigenic differentiation of vaccine strains. Journal of Hygiene 78, 429438.
Tikasingh E. S., Spence L. & Downs W. G. (1966). The use of adjuvant and sarcoma 180 cells in the production of mouse hyperimmune ascitic fluids to arboviruses. American Journal of Tropical Medicine and Hygiene 15, 219226.
Umrigar M. D. & Pavri K. M. (1977). Comparative serological studies on Indian strains of West Nile virus isolated from different sources. Indian Journal of Medical Research 65, 603612.
Yasamura U. & Kawakita Y. (1963). Studies on Sv40 in relationship to tissue culture. Nippon Rhinsho 21, 12011209.
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Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
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