Skip to main content
    • Aa
    • Aa

Apramycin resistance plasmids in Escherichia coli: possible transfer to Salmonella typhimurium in calves

  • J. E. B. Hunter (a1) (a2), J. C. Shelley (a1), J. R. Walton (a1), C. A. Hart (a2) and M. Bennett (a1)...

An outbreak of salmonellosis in calves was monitored for persistence of Salmonella typhimurium excretion in faeces and the effect of treatment with apramycin. Prior to treatment apramycin-resistant Escherichia coli were present but all S. typhimurium isolates were sensitive. Following the treatment of six calves with apramycin, apramycin-resistant S. typhimurium were isolated from two treated calves and one untreated calf. Plasmid profiles of E. coli and S. typhimurium were compared and plasmids conferring resistance to apramycin and several other antibiotics were transferred by conjugation in vitro from calf E. coli and S. typhimurium isolates to E. coli K-12 and from E. coli to S. typhimurium. The plasmids conjugated with high frequency in vitro from E. coli to S. typhimurium, and hybridized to a DNA probe specific for the gene encoding aminoglycoside acetyltransferase 3-IV (AAC(3)-IV) which confers resistance to apramycin, gentamicin, netilmicin and tobramycin.

Hide All
3. LR Ward , EJ Threlfall , B Rowe . Multiple drug resistance in salmonellae in England and Wales: a comparison between 1981 and 1988. J Clin Pathol 1990; 43: 563–6.

4. D Salauze , I Otal , R Gomez-Lus , J Davies . Aminoglycoside acetyltransferase 3–1V (aacC4) and hygromycin B4–1 phosphotransferase (hphB) in bacteria isolated from human and animal sources. Antimicrob Agents Chemother 1990; 34: 1915–20.

5. E Chaslus-Danela , G Gerbaud , JL Martel , M Lagorce , JP Lafont , P Courvalin . Probable transmission between animals of a plasmid encoding aminoglycoside 3-N-acetyltransferase IV and dihydrofolate reductase 1. Vet Microbiol 1987; 15: 97104.

9. E Chaslus-Dancla , Y Glupczynski , G Gerbaud , M Lagorce , JP Lafont , P Courvalin . Detection of apramycin resistant Enterobacteriaceae in hospital isolates. FEMS Microbiol Lett 1989; 61: 261–6.

13. PM Bennett , J Heritage , PM Hawkey . An ultra-rapid method for the study of antibiotic resistance plasmids. J Antimicrob Chemother 1986; 18: 421–4.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
Please enter your name
Please enter a valid email address
Who would you like to send this to? *


Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 3 *
Loading metrics...

Abstract views

Total abstract views: 47 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 18th October 2017. This data will be updated every 24 hours.