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The challenge of improving boiling: lessons learned from a randomized controlled trial of water pasteurization and safe storage in Peru

Published online by Cambridge University Press:  22 February 2016

Department of Epidemiology, University of Washington, Seattle, WA, USA
Bacteriology Department, U.S. Naval Medical Research Unit No. 6, Callao, Peru
Department of Epidemiology, University of Washington, Seattle, WA, USA Waterborne Disease Prevention Branch, U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA
Bacteriology Department, U.S. Naval Medical Research Unit No. 6, Callao, Peru Department of Neurology, University Of Washington, Seattle, WA, USA
Bacteriology Department, U.S. Naval Medical Research Unit No. 6, Callao, Peru
Department of Epidemiology, University of Washington, Seattle, WA, USA
Department of Environmental Health, Hospital San Juan de Dios, Ica Regional Ministry of Health, Pisco, Peru
Department of Environmental Health, Hospital San Juan de Dios, Ica Regional Ministry of Health, Pisco, Peru
Department of Epidemiology, University of Washington, Seattle, WA, USA
*Author for correspondence: Ms. K. Heitzinger, University of Washington, Department of Epidemiology, Box 357236, Seattle 98195, WA, USA. (Email:
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Boiling is the most common method of household water treatment in developing countries; however, it is not always effectively practised. We conducted a randomized controlled trial among 210 households to assess the effectiveness of water pasteurization and safe-storage interventions in reducing Escherichia coli contamination of household drinking water in a water-boiling population in rural Peru. Households were randomized to receive either a safe-storage container or a safe-storage container plus water pasteurization indicator or to a control group. During a 13-week follow-up period, households that received a safe-storage container and water pasteurization indicator did not have a significantly different prevalence of stored drinking-water contamination relative to the control group [prevalence ratio (PR) 1·18, 95% confidence interval (CI) 0·92–1·52]. Similarly, receipt of a safe-storage container alone had no effect on prevalence of contamination (PR 1·02, 95% CI 0·79–1·31). Although use of water pasteurization indicators and locally available storage containers did not increase the safety of household drinking water in this study, future research could illuminate factors that facilitate the effective use of these interventions to improve water quality and reduce the risk of waterborne disease in populations that boil drinking water.

Original Papers
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Copyright © Cambridge University Press 2016


In low- and middle-income countries (LMICs), diarrhoea is a leading cause of morbidity and mortality in children aged <5 years [Reference Walker13]. Household water treatment and safe storage are recommended as part of the WHO strategy to reduce diarrhoea risk [4], yet <30% of people living in LMICs report using an effective method to treat their household drinking water [Reference Rosa and Clasen5]. Boiling is the sole method of household treatment that has reached scale in any country, as it is the only method reported by >90% of the population in nationally representative household surveys [Reference Rosa and Clasen5]. Globally, it is also over three times more frequently practised than any other method [Reference Rosa and Clasen5]. On the basis of the demonstrated effectiveness and scalability of boiling, its improvement and expansion has been proposed as a potentially effective means of increasing household access to safe drinking water [Reference Clasen6].

Despite the relatively high acceptability of boiling, the practice has a number of potential shortcomings that may limit its effectiveness and use. In the absence of safe storage and handling in the home, boiled water supplies may be recontaminated [Reference Oswald7Reference Wright, Gundry and Conroy9]; other drawbacks include collection time and cost of fuel [Reference Gilman and Skillicorn10, Reference Biran, Abbot and Mace11], risk of scalding [Reference Samuel12Reference Delgado15], and, among populations that rely on biomass fuel, indoor air pollution [Reference Smith, Mehta, Maeusezahl-Feuz, Ezzati, Lopez and Roders16] and emission of black soot, an important contributing factor to climate change [Reference Ramanathan17, Reference Jacobson18].

There is evidence that some of the obstacles to effective boiling can be addressed by simple technological interventions. The results of randomized trials indicate that the risk of recontamination of boiled water supplies can be reduced by the use of storage containers that prevent the introduction of hands or dipping objects [Reference Roberts19Reference Ercumen21], although one study found no effect [Reference Firth22]. A study conducted in Kenya demonstrated the feasibility and effectiveness of teaching a low-income population to pasteurize their water – that is, heating water to sub-boiling temperatures to inactivate pathogens by using a thermosensitive indicator [Reference Iijima23]. Relative to boiling, pasteurization is a less time-consuming procedure and reduces the risks and potential costs associated with boiling. Among reported users in the Kenyan study, pasteurization reduced the prevalence of microbial contamination of drinking water by over 30% and the incidence of severe diarrhoea by 45% relative to individuals who drank untreated water. No other research has been conducted to assess the effect of pasteurization indicator use on household drinking-water safety.

In rural Peru, 70% of households report boiling [24], yet one investigation documented that 95% of household drinking-water samples contained microbiological contamination [Reference Miranda25], which suggests that the effectiveness of the practice is sub-optimal. We evaluated whether two simple technological interventions – an improved storage container and a water pasteurization indicator – were effective in reducing faecal contamination of household drinking water in a rural Peruvian population that boils drinking water.


Study design

We conducted an unblinded randomized controlled trial, assigning households in a 1:1:1 ratio to one of two intervention groups or to a control group. The trial was conducted between January and April 2014.

Study setting

The study was conducted in Humay district, Pisco province, on Peru's southern coast. The district is located in the Pisco River Valley and has a population of 5800 inhabitants. It receives ~2 mm of rainfall annually. The majority of households use piped drinking-water sources. Water distributed by piped systems either flows directly from the Pisco River, or indirectly through irrigation channels. The piped system of one community distributes water from a borehole. In some communities, the water from piped water systems passes through a sand or gravel filter before distribution. A member of the community water board manually chlorinates piped water stored in elevated tanks before distribution in the two largest communities; this is done on an intermittent basis due to an inconsistent supply of chlorine and lack of availability of the person responsible for chlorination. Because piped water is available between 2 and 20 h a day, many households pump water into elevated tanks above their homes in order to be able to use it outside of normal service hours.

Sample size

The sample size was calculated to detect a 15% relative reduction in the prevalence of Escherichia coli contamination in household drinking water between each of the intervention arms compared to the control arm with 95% confidence (with respect to two-sided test) and 80% power, assuming a total number of six follow-up microbiological tests of stored drinking water per household, and an estimated 88% prevalence of E. coli contamination in stored drinking water, 20% loss to follow-up, and a design effect of 2. The prevalence of E. coli contamination was estimated using pilot data collected from the study district in 2010. The design effect was included to account for the correlation between observations collected from the same household. We aimed to enrol 70 subject households per arm to ensure sufficient power to detect this difference based on these assumptions.

Participants and enrolment

As described previously [Reference Heitzinger26], households were eligible to participate in the study if they contained a woman aged ⩾18 years, a child aged <5 years, and were able to heat water in their home. Field teams conducted a census of the district before the study to identify eligible households. A computerized pseudo-random number generator was used to create a randomly ordered list of eligible houses for recruitment. Using a geographically organized list of eligible households, enumerators enrolled households to participate until a total of 210 was reached; households were randomly assigned to one of the three study groups. If a female head of household was not available at the initial visit, field workers made one additional attempt to enrol the household. Only the female head of household was enrolled for each house and she was the only household member requested to complete study procedures. All participants provided written informed consent before initiation of any study procedures.

At the enrolment visit, a baseline survey was administered to assess participant demographic characteristics; socioeconomic status; household water supply, treatment, storage, and handling behaviours; sanitation and hygiene; presence of a handwashing station (defined as a designated place for handwashing with a water supply and soap present); and ability to demonstrate correct handwashing technique (lathering all surfaces of the hands with soap). A socioeconomic index was generated using principal component analysis of household assets, building materials, and home ownership [Reference Vyas and Kumaranayake27]. The index was comprised of the first principal component of each variable [Reference Houweling, Kunst and Mackenbach28] and households were grouped into index terciles for analysis. Field workers used an orthotolidine (OTO) pool test kit (Pentair, USA) to test household source and stored drinking water for the presence of total chlorine. Source and stored household water samples were collected in sterile bottles and analysed for E. coli using the Compartment Bag Test (Aquagenx, USA); this test is a highly sensitive and specific qualitative measure [Reference Stauber29] and has a minimum threshold of detection of 1 E. coli/100 ml [30]. Samples were transported in coolers containing ice packs to the study centre and processed within 6 h of collection. All samples were incubated for a minimum of 20 h at 35–44·5 °C [30]. Positive and negative controls were incubated daily with the samples to verify the correct functioning of the tests.

Randomization and study interventions

The randomization list of eligible houses was computer-generated by the principal investigator (K.H.). Simple randomization was used to assign eligible households to study groups. The group assignments were concealed from the field team enrolling participants until completion of the baseline survey. At that time, the field team phoned the principal investigator, who opened the opaque sealed envelope containing the group assignment that corresponded to the household and communicated the assignment. At the end of the enrolment visit, all participants were informed of the dangers of drinking untreated water. Participants were randomly assigned to one of three groups. Study group A received a locally available 20-l storage container with a tight-fitting lid and spigot (which we will refer to henceforth as an ‘improved container’; Reyplast, Peru); study group B received an improved container and a water pasteurization indicator (Solar Cookers International, USA; Fig. 1) with written and pictorial instructions for its use. The indicator consists of a wax that melts at the pasteurization temperature of 65 °C in a polycarbonate tube connected to a stainless steel cable that is used to dip the indicator tube into the container in which water is being heated. Two key advantages of indicator use described in the manufacturer's instructions – fuel and time savings – were highlighted in the instructions distributed to participants. Field workers additionally performed a short demonstration of how to use the indicator to pasteurize water and asked the study participant to demonstrate correct use of the indicator in order to ensure understanding. If the participant did not initially demonstrate correct use of the indicator, the field worker reinforced training until the participant could perform the demonstration correctly. Training in indicator use was only conducted once and was not reinforced during the follow-up period. The indicators were reusable and participants were instructed to use the indicator every time they wanted to treat their drinking water. Study group C received no additional intervention and served as a control group. Participants who received an intervention were asked to use it during the course of the study for their household drinking water.

Fig. 1. Water pasteurization indicator (reproduced with permission from

Outcome assessment

The primary outcome was the presence of detectable E. coli contamination in stored drinking water. Follow-up home visits began 3 weeks following enrolment and were conducted every 2 weeks over a 10-week period, yielding a total of six possible follow-up visits and 13 weeks follow-up. At each visit, source and stored household water samples were collected and analysed for E. coli using the Compartment Bag Test following the same procedures as described for the enrolment visit.

Adherence and other measures

Field workers completed a short survey at all follow-up visits to assess cooking practices, reported and observed chlorination of source and stored water, and a proxy measure of boiling behaviour (observation of water in a pot on a stove or open fire). In the control group, water storage practices were also observed. In both intervention groups, field workers evaluated use of the improved container (defined as observation of water in the container), and the condition of the container. Participants in study group B were asked to demonstrate and explain how they use their indicator. Correct indicator use was defined as proper indicator tube placement (wax end of tube pointed upward) in a container of water being heated, along with the knowledge to stop heating water when the wax falls to the bottom of the tube. We could not directly assess indicator use because we lacked the resources to do structured observations and, because the indicator tubes were reusable, no used or discarded tubes could be observed. At the final visit, open-ended questions regarding the advantages and disadvantages of the interventions were administered to participants assigned to groups A and B.

Statistical analysis

Data were entered into Microsoft Access 2010 (Microsoft Corp., USA) and analysed using Stata v. 13.1 (StataCorp., USA). Cross-tabulations were used to examine the distribution of baseline characteristics by randomization assignment. We used generalized estimating equations (GEE) with a log link function, binomial distribution, working exchangeable correlation structure to account for correlation between repeated measures collected from the same household, and robust standard errors to compare the prevalence of E. coli contamination between paired source and stored water samples. As our primary analysis, we used an intention-to-treat approach to assess the effect of the interventions on E. coli contamination. The prevalence ratios (PRs) of E. coli contamination were estimated using a GEE model with the same specifications as above. We assessed the sensitivity of these results to possible confounding caused by imbalances in the baseline demographic and socioeconomic characteristics of the randomization groups and or by imbalances in potentially confounding follow-up variables (E. coli contamination of source water, source water chlorination, and type of cooking stove). Substantial confounders were defined as variables that altered the PR estimates by ⩾10% in adjusted models. The characteristics of participants who adhered to improved container use (had water in the container at ⩾4 follow-up visits) were evaluated using χ 2 and Fisher's exact tests, with Fisher's exact tests being used when any cell contained <5 observations [Reference Gaddis and Gaddis31]. The prevalence of E. coli contamination in stored water was compared between participants who were and were not adherent to container use within each intervention arm using GEE to adjust for household-level clustering.

Ethical considerations

This study protocol was approved by the Institutional Review Boards of the University of Washington, the U.S. Naval Medical Research Unit No. 6 (NAMRU-6), and the Ica Regional Ministry of Health. Written informed consent was obtained from all subjects and all subject personal identifiers were irreversibly removed from electronic databases following the end of data collection.



Field teams invited a total of 333 households to participate and 210 were enrolled and randomized (Fig. 2). Of the 123 households that were not enrolled, the most common reasons for non-participation were not being available at two attempts to enrol (34%), not having a child aged <5 years (26%), and refusal to participate (18%). The geographical distribution of households did not differ by enrolment status (P = 0·65). The baseline characteristics of eligible participants randomized to each of the three study groups were similar (Table 1). At baseline, E. coli was detected in 42·5% of stored water samples; chlorine was not detected in any stored water samples. Three participants were excluded from analysis post-randomization due to failure to meet study eligibility criteria. Two participants moved following the enrolment visit, resulting in a total of 205 participants who provided follow-up data for analysis.

Fig. 2. Trial profile.

Table 1. Household demographic, socioeconomic, water, sanitation, and hygiene characteristics by study arm, Pisco, Peru, 2014*

* Numbers may not sum to total due to missing values.

Overall, 59% of households used a water source that was piped to the home, 34% used an improved source not piped to the home (primarily community standpipes), and 7% used an unimproved source. Nearly all participants (98%) reported treating their household drinking water, with 99% of those doing so by boiling and 1% using both boiling and chlorination. When asked an open-ended question regarding the definition of boiling, 194 (96%) of the participants who reported boiling provided a response consistent with adequate heating for water disinfection. Eighty-nine per cent of participants stored their drinking water in a wide-mouthed container; 11% used a teapot. Storage containers were observed to be covered at 94% of enrolment visits. Thirty per cent of households dipped a hand or an object to extract water from the container.

E. coli contamination of stored drinking water

During the follow-up period, the percentages of paired stored and source water samples contaminated by E. coli were similar in group A (storage container only) and group C (the control group; P values from GEE models >0·13). In group B (storage container plus indicator), stored water samples were less likely to be contaminated than their paired source water samples (P = 0·048). In group C, 37·3% of stored water samples contained detectable E. coli, compared to 38·3% in group A, and 44·2% in group B; these differences were not statistically significant (P values for pairwise comparisons in GEE models >0·19). The PR of E. coli contamination relative to group C was 1·02 [95% confidence interval (CI) 0·79–1·31] for group A and 1·18 (95% CI 0·92–1·52) for group B (Table 2). There was no evidence of confounding of these estimates by demographic or socioeconomic factors or potentially confounding follow-up variables. The prevalence of E. coli contamination in group C was similar during baseline and follow-up visits (P = 0·14).

Table 2. Effect of interventions on the mean prevalence of Escherichia coli contamination of stored drinking water in Pisco, Peru, 2014

PR, Prevalence ratio; CI, confidence interval.

There was no trend in the prevalence of contamination of stored water over time in any of the study groups (all P values >0·12; Fig. 3). E. coli was detected in at least one follow-up visit for 167 (81%) of 205 households; of the 91 households with complete follow-up data, one household (1%) had contaminated drinking water at all six follow-up visits; ten households (11%) had no E. coli detected in stored drinking water at any of the follow-up visits, and these households were roughly equally distributed among the three study groups.

Fig. 3. Percentage of stored drinking-water samples contaminated by Escherichia coli, by study arm and follow-up visit round, Pisco, Peru, January–April 2014.

Adherence to interventions and control group practices

In the intervention groups, improved containers were observed at 97% of follow-up visits and were observed to have water inside at 55% and 58% of the follow-up visits in study groups A and B, respectively. Container usage decreased over time in both intervention groups (P values of GEE models <0·01); about 50% of households in both intervention arms were using their storage container at the final visit (Fig. 4). The indicator was observed at 90% of visits; four participants lost their indicator during the follow-up period. Knowledge of correct indicator use was demonstrated by over 90% of participants during each bi-weekly follow-up period (Fig. 4).

Fig. 4. Percentage of intervention group A and B participants using improved storage container, and intervention group B participants with knowledge of proper indicator use, by follow-up visit round, Pisco, Peru, January–April 2014.

About half (48%) of participants in both intervention groups adhered to use of the improved storage container (defined as use at ⩾4 visits). Adherent participants were more likely to have an off-premises water source (not piped directly to the participant's home, yard, or plot; 54% vs. 27%, P = 0·002). No other baseline variables were associated with adherence. In both intervention groups, participants who adhered to improved container use had similar stored water quality as participants who used their containers less frequently (Table 3).

Table 3. Mean prevalence of Escherichia coli contamination of stored drinking water by adherence with improved storage container use, Pisco, Peru, 2014

CI, confidence interval.

In group C (the control group), the storage container was covered less frequently during the follow-up period, but prevalence of container coverage was still high (86% of the follow-up visits vs. 95% of baseline visits; P = 0·003). Water was observed in a pot on the stove or open fire at 36% of visits in group C; this indicator of boiling was observed with similar frequency in group A (36%) and group B (32%).

Perceptions of the interventions

When asked to cite the advantages and disadvantages, if any, of the safe-storage container, participants in groups A and B most frequently stated that it was easy to use (53%), it made the water safer for drinking (42%), and allowed them to store water in larger quantities (31%). The most frequently cited disadvantage was that the container was too large (3%). Group B participants reported that the primary advantages of using the indicator were saving fuel (83%), saving time (29%), and ease of use (28%). Disadvantages included difficulty of use (3%) and needing to keep an eye on the indicator to use it correctly (3%).


Although the results of this trial demonstrated the feasibility of teaching a low-income population to pasteurize their household drinking water via the use of a thermosensitive indicator, this intervention had no significant effect on the risk of E. coli contamination of household drinking water. This study is the first to test the effect of use of a water pasteurization indicator in a population in which water boiling was a culturally accepted practice. In the only previous study of a similar intervention, indicator users were compared to individuals who drank untreated water [Reference Iijima23]. We found that the provision of an improved storage container had no effect on the prevalence of E. coli contamination of household drinking water, which was similar to the result of one previous trial of an improved container [Reference Firth22] but inconsistent with others that demonstrated reductions in indicators of faecal contamination [Reference Roberts19Reference Ercumen21].

The lack of effect of the pasteurization indicator intervention could be explained in several ways. First, despite retaining knowledge of how to use the indicators, participants may not have used them. Because the indicators are reusable, we could not verify this hypothesis. Second, participants may have used the indicators and this behaviour may have resulted in more effective treatment, but inconsistency of use prevented any significant reduction in contamination. The observation of the proxy for boiling – water in a pot on a stove or fire – at over 30% of home visits coupled with the significant reduction in contamination from source to stored water in group B supports this hypothesis, although we could not confirm the effectiveness of treatment. We could also not confirm boiling behaviour and our proxy for boiling may have been inaccurate if water was boiled outside of the daytime hours during which field workers conducted the study visits. An alternative explanation for the null effect is that although use of the pasteurization indicator resulted in more effective treatment behaviour, the receipt of an indicator led participants to reduce their investment in other protective behaviours such as handwashing, thus negating the impact of more effective treatment on water quality. The effect of the prevalence of an exposure such as a public health intervention on demand for disease prevention – termed prevalence elasticity – has been observed for other health-related outcomes [Reference Philipson, Culyer and Newhouse32], but because we did not assess preventive behaviours including hygiene or safe water handling at follow-up visits, we could not confirm whether this occurred. Unlike the previous study using a water pasteurization indicator intervention [Reference Iijima23], we did not employ female community leaders to promote indicator use, nor did we demonstrate the effectiveness of pasteurization in reducing the number of bacterial colonies on an agar plate. It is possible that these two elements were critical in the promotion of indicator use and should be considered in future trials of this intervention in order to increase effectiveness. In group A, generally safe water storage and handling practices and good hygiene in the control group likely limited our ability to detect a benefit of the intervention.

The results of this study demonstrate the challenge of improving boiling and safe water storage in a population in which both practices have been accepted but perhaps inconsistently or ineffectively practised. Although participants generally expressed satisfaction with the container, these stated perceptions likely overstated actual satisfaction due to courtesy bias. Moderate use of the storage container highlighted the importance of identifying interventions that the population perceives as offering a relative advantage over current storage practices to increase the probability of use [Reference Everett33]. Similarly, although most group B participants exhibited knowledge of indicator use and perceived its advantages, we could not confirm indicator use. The lack of effect of the indicator may have reflected lack of use for similar reasons as for the storage container.

This study had two important limitations. First, we based our sample size calculation on the prevalence of E. coli contamination in drinking-water samples collected from the same district in 2010, as this was the only relevant data available at the time of protocol development. Although the majority of drinking water from even piped sources in rural Peru is contaminated with E. coli [Reference Miranda25, Reference Bain34], progressive expansion of access to piped water infrastructure in the area likely contributed to the unexpectedly low prevalence of contamination in the control group, which limited our ability to detect statistically significant differences. Second, we assessed our primary outcome, E. coli contamination, using the Compartment Bag Test. This test has the advantages of requiring minimal materials or technical skill to perform, but yields imprecise quantitative results [30]. It is, however, a highly sensitive and specific qualitative measure of contamination [Reference Stauber29]. A qualitative assessment of E. coli contamination may be adequate given that it corresponds with the WHO standard of drinking-water safety [35]. Moreover, the results of a recent systematic review and meta-analysis indicate that while the presence of E. coli contamination in drinking water is associated with the risk of diarrhoea, increasing levels of E. coli contamination in drinking water beyond a detectable level did not demonstrate a dose-response relationship with risk [Reference Gruber, Ercumen and Colford36]. This finding was based on a small number of studies, however, and more research is needed to determine whether a qualitative measure of E. coli contamination is sufficient indicator of health risk.

Our study findings indicate that the use of a water pasteurization indicator was not effective in reducing faecal contamination of household drinking water in a population that had already adopted boiling as a method of treatment. However, this investigation and a previous study [Reference Iijima23] provided evidence of the feasibility of teaching a low-income population to use a thermosensitive indicator to pasteurize their drinking water. Future research is needed on methods to improve objective measurement of indicator use and assess the impact of this intervention on drinking water safety and diarrhoeal disease in populations at high risk for waterborne disease. Formative research may additionally be useful to better understand the factors that influence use of water treatment and storage interventions designed to limit the contamination of drinking water in the home.


We thank the study participants for their contribution to this investigation. This research would not have been possible without the administrative and logistical support of Asociación Benéfica PRISMA, particularly Crisóloga Lauro Salas, Angela Huamán Gómez, Alex Fernández Díaz, Enny Herrera Mayuri, Beatriz Huamán Ccollana, and Aydee Huanaco Muñoz. We additionally thank the Peace Corps Water and Sanitation Programme in Peru, particularly Hilary Miller, for her help throughout this project.

This research was supported by the Department of Defense Humanitarian Assistance Program (project no. 20306) and by NIH research training grant no. R25 TW009345 awarded to the Northern Pacific Global Health Fellows Program by the Fogarty International Center.

C. A. Rocha, R. E. Quick, S. M. Montano and D. H. Tilley Jr. are employees of the U.S. Government. This work was prepared as part of their official duties. The findings and conclusions expressed in this manuscript are those of the authors and do not necessarily reflect the official policy or position of the Department of the Navy, Department of Defense, the Centers for Disease Control and Prevention, nor the U.S. government.





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Figure 0

Fig. 1. Water pasteurization indicator (reproduced with permission from

Figure 1

Fig. 2. Trial profile.

Figure 2

Table 1. Household demographic, socioeconomic, water, sanitation, and hygiene characteristics by study arm, Pisco, Peru, 2014*

Figure 3

Table 2. Effect of interventions on the mean prevalence of Escherichia coli contamination of stored drinking water in Pisco, Peru, 2014

Figure 4

Fig. 3. Percentage of stored drinking-water samples contaminated by Escherichia coli, by study arm and follow-up visit round, Pisco, Peru, January–April 2014.

Figure 5

Fig. 4. Percentage of intervention group A and B participants using improved storage container, and intervention group B participants with knowledge of proper indicator use, by follow-up visit round, Pisco, Peru, January–April 2014.

Figure 6

Table 3. Mean prevalence of Escherichia coli contamination of stored drinking water by adherence with improved storage container use, Pisco, Peru, 2014