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Comparison in the immunological properties of Borrelia burgdorferi isolates from Ixodes ricinus derived from three endemic areas in Switzerland

Published online by Cambridge University Press:  15 May 2009

C. M. Hu ,
S. Leuba-Garcia ,
M. D. Kramer ,
A. Aeschlimann  and
L. Gern
C. M. Hu
Affiliation:
Institut de Zoologie, Neuchâtel University, CH-2000 Neuchâtel, Switzerland
S. Leuba-Garcia
Affiliation:
Institut de Zoologie, Neuchâtel University, CH-2000 Neuchâtel, Switzerland
M. D. Kramer
Affiliation:
Institut für Immunologie, Ruprecht-Karls-Universität, Heidelberg, Germany
A. Aeschlimann
Affiliation:
Institut de Zoologie, Neuchâtel University, CH-2000 Neuchâtel, Switzerland
L. Gern*
Affiliation:
Institut de Zoologie, Neuchâtel University, CH-2000 Neuchâtel, Switzerland
*
* Author for correspondence: Institut de Zoologie, Chantemerle 22, CH-2000 Neuchâtel, Switzerland.
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Borrelia burgdorferi isolates were obtained from Ixodes ricinus from three sites in Switzerland. They were examined by SDS-PAGE and immunoblotting. The phenotypes, in respect of three outer surface proteins (Osp), differed between the sites of collection. In site 1, most isolates had an OspA of 31 kDa and an OspB of 34 kDa: in site 2, isolates presenting an OspA of 33 kDa dominated and in site 3, the isolates with an OspA of 32 kDa and an OspB of 35 kDa were most frequent. This distribution differed significantly. About half of the isolates from sites 1 and 3 reacted with anti-OspA monoclonal antibody H5332 compared to 29% from site 2. Site 1 isolates reacted significantly more frequently (81 %) with another anti-OspA monoclonal antibody LA-31 than isolates from site 3 (P < 0·0001). These findings have implications for the epidemiology of Lyme borreliosis, for the further development of serodiagnostic reagents and for the development of a vaccine.

Type
Research Article
Information
Epidemiology & Infection , Volume 112 , Issue 3 , June 1994 , pp. 533 - 542
Copyright
Copyright © Cambridge University Press 1994

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