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A comparison of enrichment media for the isolation of salmonellae from seagull cloacal swabs

Published online by Cambridge University Press:  19 October 2009

C. R. Fricker
Affiliation:
Scottish Salmonella Reference Laboratory, Stobhill General Hospital, Glasgow G21 3UW
R. W. A. Girdwood
Affiliation:
Scottish Salmonella Reference Laboratory, Stobhill General Hospital, Glasgow G21 3UW
D. Munro
Affiliation:
Scottish Salmonella Reference Laboratory, Stobhill General Hospital, Glasgow G21 3UW
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The relative efficiency of three selective enrichment broths (Muller-Kauffmann tetrathionate, Rappaport's and selenite F) was investigated for the isolation of salmonellae from seagull cloacal swabs. Pre-enrichment in buffered peptone water was employed throughout the study, which involved the examination of 560 gulls, sixty (10·7%) of which were found to be carrying salmonellae. Rappaport's broth as modified by Vassiliadis for incubation at 43°C (Vassiliadis et al. 1976) yielded the highest number of positive swabs (57) and the widest range of serotypes. It was significantly more efficient that either selenite F or tetrathionate broth, although the results obtained with Rappaport's broth incubated at 37 and 43°C did not differ significantly (P > 0·5). Eleven serotypes were isolated during, the study, the most prevalent being Salmonella virchow.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1983

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