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Complexity of Pseudomonas aeruginosa infection in cystic fibrosis: combined results from esterase electrophoresis and rDNA restriction fragment length polymorphism analysis

  • E. Denamur (a1), B. Picard (a2), Ph. Goullet (a2), E. Bingen (a3), N. Lambert (a3) and J. Elion (a1)
  • DOI: http://dx.doi.org/10.1017/S0950268800067595
  • Published online: 19 October 2009
Abstract
SUMMARY

Esterase electrophoretic typing and restriction fragment length polymorphism of ribosomal DNA regions (ribotyping) were used to differentiate 102 Pseudomonas aeruginosa clinical isolates obtained from chronic lung infection in 23 patients with cystic fibrosis (CF) and two reference strains (including the type strain ATCC 10145). Twenty-five zymotypes were obtained with the former method and 16 ribotypes with the latter. Combination of the two typing systems led to the finding of 30 different types. Our data highlights the physiopathological complexity of P. aeruginosa infection in CF as, in six individual cases, several types were found among isolates from a given patient. On the other hand, two unique types were found in two and three patients respectively, raising the possibility of crossinfections.

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1.TL Pitt . Epidemiological typing of Pseudomonas aeruginosa. Eur J Clin Microbiol Infect Dis 1988; 7: 238–47.

9.S Pattyn , G. Mertens Esterase iso-enzyme electrophoresis for epidemiological surveillance of Pseudomonas aeruginosa hospital infections. Eur J Clin Microbiol 1988; 7: 821–2.

13.ES. Lennox Transduction of linked genetic characters of the host by bacteriophage PI. Virol 1955; 1: 190206.

14.PH. Goullet An esterase zymogram of Escherichia coli. J Gen Microbiol 1973; 77: 2735.

16.PH Goullet , B. Picard A two-dimensional electrophoretic profile for bacterial esterases. Electrophoresis 1985; 6: 132–5.

17.SH Lawrence , PJ Melnick , HE Weimer . A comparison of serum proteins and enzymes by starch-gel electrophoresis. Proc Soc Experim Biol Med 1960; 105: 572–5.

19.N Picard-Pasquier , Ouagued'M, B Picard , PH Goullet , R. Krishnamoorthy A simple sensitive method of analyzing bacterial ribosomal DNA polymorphism. Electrophoresis 1989; 10: 186–9.

20.EM. Southern Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 1975; 98: 503–17.

27.B Picard , B Bruneau , PH. Goullet Demonstration of an outbreak of Serratia marcescens infections in a medical intensive care unit by esterase electrophoretic typing. J Hosp Infect 1988; 11: 194–5.

29.F Grimont , PAD. Grimont Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur/Microbiol 1986; 137B: 165–75.

30.TL Stull , JJ Lipuma , TD Edlind . A broad spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. J Infect Dis 1988; 157: 280–86.

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Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
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