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Detection of Q fever antibodies in whey by the anti-globulin sensitization test and other techniques

Published online by Cambridge University Press:  15 May 2009

M. G. P. Stoker  and
B. P. Marmion
M. G. P. Stoker
Affiliation:
Department of Pathology, University ofCambridge, and the Virus Reference Laboratory, Colindale
B. P. Marmion
Affiliation:
Department of Pathology, University ofCambridge, and the Virus Reference Laboratory, Colindale
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1. Samples of whey from individual cows and herds infected with R. burneti and from uninfected cows and herds have been tested for R. burneti antibodies.

2. The haemolytic complement-fixation test, the conglutinating complement-absorption test, the direct agglutination test and the anti-globulin sensitization test were compared for sensitivity in detecting whey antibody.

3. The direct agglutination test was generally more sensitive than either of the complement-fixation tests, particularly when it was enhanced by centrifugation of the sensitized rickettsiae. The anti-globulin sensitization test, however, gave the highest titres andit appeared to be specific.

4. The practical application of these techniques and certain theoretical considerations are discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 50 , Issue 1 , March 1952 , pp. 1 - 11
Copyright
Copyright © Cambridge University Press 1952

References

REFERENCES

Blomfield, A. M., Coombs, R. R. A. & Hole, N. H. (1950). The conglutination phenomenon. VI. An experimental investigation of the factors determining the adsorption of complement by an antigen-antiserum mixture. J. Hyg., Camb. 48, 73.CrossRefGoogle ScholarPubMed
Coombs, R. R. A., Mourant, A. E. & Race, R. R. (1945). A new test for the detection of weak and ‘incomplete’ Rh agglutinins. Brit. J. exp. Path. 26, 255.Google ScholarPubMed
Coombs, R. R. A. & Stoker, M. G. P. (1951). Detection of Q fever antibodies by the anti-globulin sensitisation test. Lancet, ii, 15.CrossRefGoogle Scholar
Hole, N. H. & Coombs, R. R. A. (1947). The conglutination phenomenon. I. An introduction to the conglutination phenomenon and an account of the observations and views of previous investigators. J. Hyg., Camb. 45, 480.Google Scholar
Jones, L. O. & Wilson, M. M. (1951). Serum agglutinins in brucellosis. Nature, Lond. 167, 558.CrossRefGoogle ScholarPubMed
Marmion, B. P. & Stoker, M. G. P. (1950). Q fever in Great Britain. Lancet, ii, 611.CrossRefGoogle Scholar
Morgan, W. T. J. & Schütze, H. (1946). Non-agglutinating antibody in human antisera to Sh. shiga and S. typhi. Brit. J. exp. Path. 27, 286.Google Scholar
Plotz, H. (1943). Complement fixation in rickettsial diseases. Science, 97, 20.CrossRefGoogle ScholarPubMed
Smith, E. L. & Holm, A. (1948). The transfer of immunity to the newborn calf from colostrum. J. biol. Chem. 175, 349.CrossRefGoogle Scholar
Stewart, F. S. & McKeever, J. D. (1950). The antiglobulin technique applied to the detection of non-agglutinating antibody against Salmonella typhi O in human sera. J. Hyg., Camb., 48, 357.CrossRefGoogle Scholar
Stoker, M. G. P., Coombs, R. R. A. & Bedson, S. P. (1950). The application of the con-glutinating complement-absorption test to virus systems. Brit. J. exp. Path. 31, 217.Google ScholarPubMed
Stoker, M. G. P., & Marmion, B. P. (1950). A comparison of the conglutinating complement-absorption test and the haemolytic complement-fixation test in the serological diagnosis of influenza. Brit. J. exp. Path. 31, 217.Google ScholarPubMed
Wolfe, D. M. & Kornfeld, L. (1948). Conglutinating complement-absorption test compared with haemolytic complement-fixation reactions using Q fever immune bovineserum. Proc. Soc. exp. Biol. N.Y. 69, 251.CrossRefGoogle Scholar
Wolfe, D. M. & Kornfeld, L. (1949). The application of a quantitative complement-fixation method to astudy of Q fever strain differentiation. J. Immunol. 61, 297.CrossRefGoogle Scholar