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Determination of colonization resistance of the digestive tract by biotyping of Enterobacteriaceae

  • H. Z. Apperloo-Renkema (a1), B. D. Van der Waaij (a1) and D. Van der Waaij (a1)
Summary
SUMMARY

In studies concerning the effect of antibiotics on faecal microflora, Colonization Resistance is an important parameter. Colonization Resistance correlates inversely with the number of different biotypes of Enterobacteriaceae isolated from faecal samples. Nine healthy volunteers were studied during 6 weeks, in order to determine the natural variation in the number of different biotypes of Enterobacteriaceae per faecal sample. The numbers of biotypes ranged from 1–15 per faecal sample, the mean number of biotypes varied between 2·6 and 7·3 different biotypes per faecal sample per healthy volunteer. Inter-individual variations of five biotypes in the mean number of biotypes per faecal sample are normal. We assessed the minimal number of faecal samples that should be taken for comprehensive biotyping so as to determine reliably the mean number of different biotypes representative for the Colonization Resistance of an individual. It was found that a minimum of four faecal samples was required.

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1.Hentges DJ. Que JU. Casey SW. Stein AJ. The influence of streptomycin on colonization resistance in mice. Microecol Ther 1984; 14: 5362.
2.Hofstra W. Welling GW, Waaij D van der. A comparative study of the effect of oral treatment with augmentin, amoxycillin and bacampicillin in the faecal flora in mice. Zentralbl Bakteriol Mikrobiol Hyg [A] 1988; 269: 7885.
3.Waaij D van der. The digestive tract in immunocompromised patients: importance of maintaining its resistance to colonisation, especially in hospital inpatients and those taking antibiotics. In: Sabath LD, ed. Action of antibiotics in patients. Bern: Hans Hubert Publishers. 1982: 104–18.
4.Waaij D van der. Speltie TM, Vossen JM. Biotyping of Enterobacteriaceae as a test for the evaluation of isolation systems. J Hyg 1972; 70: 639–50.
5.Waaij D van der, Speltie TM, Guinee PAM, Agterberg C. Serotyping and biotyping of 160 EschericMa coli strains: comparative study. J Clin Microbiol 1975; 1: 237–8.
6.Waaij D van der, Tieleman-Speltie TM. De Roeck-Houben AMJ. Infection by and distribution of biotypes of Enterobacteriaceae species in leukemic patients treated under ward conditions and in units for protective isolation in seven hospitals in Europe. Infection 1977; 3: 188–94.
7.Waaij D van der, Berghuis-de Vries JM, Lekkerkerk-van der Wees JEC. Colonization resistance of the digestive tract in conventional and antibiotic-treated mice. J Hyg 1971; 69:405–11.
8.Waaij D van der. Berghuis JM. Determination of the colonization resistance of the digestive tract of individual mice. J Hyg 1974; 72: 379–87.
9.Hahn DM, Schimpff SC, Fortner CL, Smyth AC, Young VM, Wiernik PH. Infection in acute leukemia patients receiving oral nonabsorbable antibiotics. Antimicrob Agents Chemother 1978: 13: 958–64.
10.Waaij D van der, Tieleman-Speltie TM, De Roeck-Houben AMJ. Infection by and distribution of biotypes of Enterobacteriaceae species in leukaemic patients treated under ward conditions and in units for protective isolation in seven hospitals in Europe. Infection 1977; 5: 188–94.
11.Waaij D van der, Tieleman-Speltie TM, De Roeck-Houben AMJ. Relation between the faecal concentration of various potentially pathogenic microorganisms and infections in individuals (mice) with severely decreased resistance to infection. Antonie van Leeuwenhoek 1978; 44: 395405.
12.Tancrède CH. Andremont AO. Bacterial translocation and gramnegative bacteremia in patients with hematological malignancies. J Infect Dis 1985; 152: 99103.
13.Schimpff SC, Young VM, Greene WH, Vermeulen GD. Moody MR. Wiernik PH. Origin of infection in acute nonlymphocytic leukemia. Significance of hospital acquisition of potential pathogens. Ann Intern Med 1972; 77: 707–14.
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Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
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