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The development and evaluation of a μ-capture ELISA detecting chlamydia-specific Igm

  • T. G. Wreghitt (a1), V. J. Robinson (a2), E. O Caul (a3), I. D. Paul (a3) and S. Gatley (a4)...

A μ-capture enzyme-linked immunosorbent assay (ELISA) for detecting chlamydia-specific IgM was developed by use of the heat stable, lipopolysaccharide group-specific antigen and an alkaline phosphatase-labelled anti-chlamydia group-specific monoclonal antibody conjugate. The test was used to study the serological response in chlamydial respiratory tract infection among patients with acute respiratory tract symptoms in Cambridgeshire during the past 7 years. Results were compared with those of the complement fixation test (CFT) in routine use as well as those of a whole inclusion indirect immunofluorescence (WIF) test for IgM. Correlation between results of the μ-capture ELISA and those of the WIF test was 87·5%.

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Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
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