Skip to main content
×
Home

The development and evaluation of a μ-capture ELISA detecting chlamydia-specific Igm

  • T. G. Wreghitt (a1), V. J. Robinson (a2), E. O Caul (a3), I. D. Paul (a3) and S. Gatley (a4)...
Summary
SUMMARY

A μ-capture enzyme-linked immunosorbent assay (ELISA) for detecting chlamydia-specific IgM was developed by use of the heat stable, lipopolysaccharide group-specific antigen and an alkaline phosphatase-labelled anti-chlamydia group-specific monoclonal antibody conjugate. The test was used to study the serological response in chlamydial respiratory tract infection among patients with acute respiratory tract symptoms in Cambridgeshire during the past 7 years. Results were compared with those of the complement fixation test (CFT) in routine use as well as those of a whole inclusion indirect immunofluorescence (WIF) test for IgM. Correlation between results of the μ-capture ELISA and those of the WIF test was 87·5%.

Copyright
References
Hide All
Berman S. L., Freundlich E., Glaser K., Abramov A. & Ephrati-Elizar E. (1955). Ornithosis in infancy. Pediatrics 15, 752759.
Bradstreet C. M. P. & Taylor C. E. D. (1962). Technique of complement fixation test applicable to the diagnosis of virus diseases. Monthly Bulletin of the Ministry of Health and Public Health Laboratory Service 21, 96104.
Byrom N. P., Walls J. & Mair H. J. (1979). Fulminant psittacosis. Lancet i, 353356.
Campbel L. A., Kuo C. C. & Grayston J. T. (1987). Charaterization of the chlamydia hybridization. Journal of Clinical Microbiology 25, 19111916.
Conway D., Glazener C. M. A., Caul E. O., Hodgson J., Hull M. G. R., Clarke S. J. R. & Stirrat G. M. (1984). Chlamydial serology in fertile and infertile women. Lancet i, 191193.
Grayston J. T., Kuo C. C., Wang S. & Altman J. (1986). A new Chalamydia psittaci strain, TWAR, isolated in acute respiratory tract infections. New England Journal of Medicine 315, 161168.
Ishikawa E., Imagawa M., Hashida S., Yoshitake S., Yamaguchi Y. & Veno T. (1983). Enzyme labelling of antibodies and their fragments for enzyme immunoassay and immunohistochemical staining. Journal of Immunology 4, 209227.
Kuo C. C., Chen H. H., Wang S. P. & Grayston J. T. (1986). Indentification of a new group of Chlamydia psittaci strains called TWAR. Journal of Clinical Microbiology 24, 10341037.
Lewis V. J., Thacker W. L. & Mitchell S. H. (1977). Enzyme-linked immunosorbent assay for chlamydial antibodies. Journal of Clinical Microbiology 6, 507510.
Macfarlane J. T. & Macrae A. D. (1983). Psittacosis. British Medical Bulletin 39, 163167.
Mcgivern D., White R., Paul I. D., Caul E. O., Room A. P. C. H. & Westmorland D. (1988). Concomitant zoonotic infection with ovine chlamydia and Q fever in pregnancy: Clinical features, diagnosis, management and public health implications. British Journal of Obstetrics and Gynaecology 95, 111115.
Meyer K. F. & Eddie B. (1956). The influence of tetracycline compounds on the development of antibodies in psittacosis. Annual Review of Tuberculosis and Pulmonary Disease 74, 566571.
Meyer K. F., Eddie B. & Schachter J. (1969). Psittacosis-lymphogranuloma venereum agents. In Diagnostic Procedures for Viral and Rickettsial Infections, 4th edition, p. 900. New York: American Public Health Association Inc.
Moule J. H., Caul E. O. & Wreghitt T. G. (1987). The specific IgM response to Mycoplasma pneumoniae infection: interpretation and application to early diagnosis. Epidemiology and Infection 99, 685692.
Nagington J. (1984). Psittacosis ornithosis in Cambridgeshire, 1975–1983. Journal of Hygiene 92, 919.
Palmer S. R., Andrews B. E. & Major R. (1981). A common-source outbreak of ornithosis in veterinary surgeons. Lancet ii, 798799.
Richmond S. J. & Caul E. O. (1982). Antibodies to Chalmydia: A Single Antigen Test. In Immunofluorescence Techniques in Diagnostic Microbiology (ed. Edwards I. M. B.Taylor C. E. D.Tomlinson A. H.), pp. 7784. PHLS Monograph Series 18. London: Her Majesty's Stationery Office.
Schachter J., & Dawson C. (1978). Human Chlamydial Infections. Massachussetts: PSG.
Schachter J., Lum L., Gooding C. A. & Ostler B. (1975). Pneumonitis following inclusion blennorhea. Journal of Pediatrics 87, 779780.
Strauss J. (1967). Microbiologic and epidemiologic aspects of duck ornithosis in Czechoslovakia. American Journal of Opthalmology 63, 12461259.
Terho P. & Meurman O. (1981). Chlamydial serum IgG, IgA and local IgA antibodies in patients with genital-tract infections, measured by solid-phase radioimmunoassay. Journal of Medical Microbiology 14, 7787.
Wang S. P. & Grayston J. T. (1971). In Trachoma and Related Disorders (ed. R. L. Nichols), pp. 217232. Amsterdam: Excerpta Medica.
Wang S.-P.Grayston J. T., Kuo C.-C.Alexander E. R. & Holmes K. K. (1977). Serodiagnosis of chlamydia trachomatis infection with the microimmunofluorescence test. In Non–gonococcal Urethritis and Related Infections (ed. Hobson D.Holmes K. K.) pp. 237248. Washington D.C.: American Society for Microbiology.
Wreghitt T. G. & Sillis M. (1985). A μ-capture ELISA for detecting Mycoplasma pneumoniae IgM: comparison with indirect immunofluorescence and indirect ELISA. Journal of Hygiene 94, 217227.
Wreghitt T. G. & Sillis M. (1987). An investigation of the Mycoplasma pneumoniae infections in Cambridge in 1983 using μ-capture ELISA, indirect immunofluorescence and complement fixation tests. Israel Journal of Medical Sciences 23, 704708.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 5 *
Loading metrics...

Abstract views

Total abstract views: 18 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 21st November 2017. This data will be updated every 24 hours.