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The development and performance of a simple, sensitive method for the detection of Cryptosporidium oocysts in faeces

Published online by Cambridge University Press:  19 October 2009

Derrick Baxby ,
N. Blundell  and
C. A. Hart
Derrick Baxby
Affiliation:
University Department of Medical Microbiology, Duncan Building, Royal Liverpool Hospital, Liverpool L7 8XW
N. Blundell
Affiliation:
University Department of Medical Microbiology, Duncan Building, Royal Liverpool Hospital, Liverpool L7 8XW
C. A. Hart
Affiliation:
University Department of Medical Microbiology, Duncan Building, Royal Liverpool Hospital, Liverpool L7 8XW
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Features are described of a new staining method for the detection of Cryptosporidium oocysts in faeces. Smears, fixed in acid-methanol, are stained with heated safranin and counterstained with methylene blue. Oocysts stain a vivid orange-pink and are easily recognized. The method is rapid and simple with little source of error. The method is more sensitive than currently recommended Ziehl-Neelson methods; of 26 cases of cryptosporidiosis diagnosed by the new method only 19 were detected by acid-fast and only 11 by acid-alcohol-fast methods. Oocysts can be stained by safranin-methylene blue after concentration by various methods, in paraffin-embedded material, and after storage for months at various temperatures.

Information

Type
Research Article
Information
Journal of Hygiene , Volume 93 , Issue 2 , October 1984 , pp. 317 - 323
Copyright
Copyright © Cambridge University Press 1984

References

Adam, K. M. G., Paul, J. & Zaman, V. (1971). Medical and Veterinary Parasitology, 1st ed., pp. 167–168. Edinburgh & London: Churchill Livingstone.Google Scholar
Angus, K. W. (1983). Cryptosporidiosis in man, domestic animals and birds: a review. Journal of the Royal Society of Medicine 76, 62–70.Google Scholar
Angus, K. W., Campbell, I., Gray, E. W. & Sherwood, D. (1981). Staining of faecal yeasts and Cryplosporidium oocysts. Veterinary Record 108, 173.Google Scholar
Baxby, D. & Blundell, N. (1983). Sensitive, rapid, simple methods for detecting Cryptosporidium in faeces. Lancet ii, 1149.Google Scholar
Baxby, D., Hart, C. A. & Taylor, C. (1983). Human cryptosporidiosis: a possible case of hospital cross-infection. British Medical Journal 287, 1760–1761.Google Scholar
Casemore, D. P., Armstrong, M.Jackson, B. (1984). Screening for Cryptosporidium in stools. Lancet i, 734–735.Google Scholar
Current, W. L., Reese, N. C., Ernst, J. V., Bailey, W. S., Heyman, M. B. & Weinstein, W. M. (1983). Human cryptosporidiosis in immunocompetent and immunodeficient persons. New England Journal of Medicine 308, 1252–1257.Google Scholar
Editorial. (1984). Cryptosporidiosis. Lancet i, 492–493.Google Scholar
Garcia, L. S., Bruckner, D. A., Brewer, T. C. & Shimizu, R. Y. (1983). Techniques for recovery and identification of Cryptosporidiumoocysts from stool specimens. Journal of Clinical Microbiology 18, 185–189.Google Scholar
Henriksen, S. A. & Pohlenz, J. F. L. (1981). Staining of cryptosporidia by a modified Ziehl-Neelson technique. Acta veterinaria scandinavica 22, 594–596.CrossRefGoogle Scholar
Ma, P. & Soave, R. (1983). Three-step stool examination for cryptosporidiosis in 10 homosexual men with protracted watery diarrhea. Journal of Infectious Diseases 147, 824–828.Google Scholar
Miller, R. A., Holmberg, R. E. & Clausen, C. R. (1983). Life-threatening diarrhea caused by Cryptosporidium in a child undergoing therapy for lymphocytic leukemia. Journal of Pediatrics 103, 256–259.Google Scholar
Nichols, G. & Thom, B. T. (1984). Screening for Cryptosporidium in stools. Lancet i, 735.Google Scholar
Pitlik, S. D., Fainstain, V., Garza, D., Guarda, L.Bolivar, R., Rios, A., Hopfer, R. L. & Mansell, P. (1983). Human cryptosporidiosis: spectrum of disease. Archives of Internal Medicine 143, 2269–2275.Google Scholar
Schultz, M. G. (1983). Editorial – emerging zoonoses. New England Journal of Medicine 308, 1285–1286.Google Scholar
Tzipori, S. (1983). Cryptosporidiosis in animals and man. Microbiological Reviews 47, 84–96.CrossRefGoogle Scholar
Tzipori, S., Angus, K. W., Gray, E. W. & Campbell, I. (1980). Vomiting and diarrhea associated with cryptosporidial infection. New England Journal of Medicine 303, 818.Google Scholar
Tzipori, S., Smith, M., Birch, C., Barnes, G. & Bishop, R. (1983). Cryptosporidiosis in hospital patients with gastroenteritis. American Journal of Tropical Medicine and Hygiene 32, 931–934.Google Scholar