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Diagnostic assays with monoclonal antibodies for the human serum parvovirus-like virus (SPLV)

Published online by Cambridge University Press:  19 October 2009

B. J. Cohen ,
P. P. Mortimer  and
M. S. Pereira
B. J. Cohen
Affiliation:
PHLS Virus Reference Laboratory, Central Public Health Laboratory, Colindale Avenue, London NW9
P. P. Mortimer
Affiliation:
PHLS Virus Reference Laboratory, Central Public Health Laboratory, Colindale Avenue, London NW9
M. S. Pereira
Affiliation:
PHLS Virus Reference Laboratory, Central Public Health Laboratory, Colindale Avenue, London NW9
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Monoclonal antibodies to the serum parovirus-like virus (SPLV) were prepared by the hybridoma technique. They provided an antibody reagent which was used to develop solid phase antibody-capture assays for anti-SPLV IgM and IgG and for SPLV antigen. These assays were more sensitive than those based on human convalescent antibody as a reagent, and were more economical in the use of SPLV antigen. Their use enabled the serological responses to SPLV to be studied more fully and their sensitivity revealed the extent of SPLV infection.

SPLV antigen was detected in four patients by both counter-immuno electrophoresis (CIE) and radioimmunoassay (RIA) and in two others by RIA alone. Parvovirus particles were seen in all six by electron microscopy. The anti-SPLV IgM response was measured in patients infected by SPLV. It was strong 5–18 days after the onset of illness, then declined and was only detectable in trace amounts after 6 months. Anti-SPLV IgG was also formed early, and persisted for at least 6 months. In a survey of 310 blood donors anti-SPLV was detected in 134 (43%) by CIE, but in 190 (61 %) by IgG antibody capture RIA.

Information

Type
Research Article
Information
Journal of Hygiene , Volume 91 , Issue 1 , August 1983 , pp. 113 - 130
Copyright
Copyright © Cambridge University Press 1983

References

REFERENCES

Anderson, M. J. (1982). The emerging story of a human pnrovirus-like agent. Journal of Hygiene 89, 1–8.CrossRefGoogle ScholarPubMed
Anderson, M. J., Davis, L. R., Jones, S. E., Pattison, J. R. & Serjeant, G. R. (1982). The development and use of an antibody capture radioimmunoassay for specific IgM to a human parvovirus-like agent. Journal of Hygiene 88, 309–324.CrossRefGoogle ScholarPubMed
Cohen, B. J., Hewish, R. A. & Mortimer, P. P. (1981). Comparison of radioimmunoassay and counter-immunoelectrophoresis for the detection of antibody to hepatitis B core antigen. Journal of Virological Methods 2, 181–192.Google Scholar
Cossart, Y. E., Field, A. M., Cant, B. & Widdows, D. (1975). Parvovirus-like particles in human sera. Lancet i, 72–73.Google Scholar
Flehmig, B., Ranke, M., Berthold, H. & Gerth, H. J. (1979). A solid phase radioimmunoassay for detection of IgM antibodies to hepatitis A virus. Journal of Infectious Diseases 140, 169–175.CrossRefGoogle ScholarPubMed
Kelleher, J. F., Luhan, N. L. C., Mortimer, P. P. & Kanimura, T. (1983). The human serum 'parvovirus': A specific cause of aplastic crisis in hereditary spherocytosis. Journal of Paediatrics (In the Press.)Google Scholar
Köhler, G. & Milstein, C. (1976). Derivation of specific antibody-producing tissue culture and tumour lines by cell fusion. European Journal of Immunology 6, 511–519.CrossRefGoogle ScholarPubMed
Pavek, W. K. & Clarke, S. K. R. (1976). Comparison of Human Fecal and Serum Parvo-like Viruses. Journal of Clinical Microbiology 4, 67–70.Google Scholar
Serjeant, G. R., Mason, K., Topley, J. M., Serjeant, B. E., Pattison, J. R.Jones, S. E. & Mohamed, R. (1981). Outbreak of aplastic crises in sickle cell anaemia associated with parvovirus-like agent. Lancet ii, 595–597.CrossRefGoogle Scholar
Shneerson, J. M., Mortimer, P. P. & Vandervelde, E. M. (1980). Febrile illness due to a parvovirus. British Medical Journal 280, 1580.CrossRefGoogle ScholarPubMed
Tedder, R. S. & Wilson-Croome, R. (1980). Detection by radioimmunoassay of IgM class antibody to hepatitis B core antigen: a comparison of two methods. Journal of Medical Virology 6, 235–247.CrossRefGoogle ScholarPubMed
Tedder, R. S., Yao, J. L. & Anderson, M. J. (1982). The production of monoclonal antibodies to rubella haemagglutinin and their use in antibody-capture assays for rubella specific IgM. Journal of Hygiene 88, 335–350.CrossRefGoogle ScholarPubMed