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Enzyme immunoassay using BCG in serodiagnosis of pulmonary tuberculosis

Published online by Cambridge University Press:  19 October 2009

F. L. Garcia-Carreñno ,
R. E. Carvajal  and
R. Hernandez
F. L. Garcia-Carreñno
Affiliation:
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Apartado Postal 2-191, 44270 Guadalajara, Jalisco, Mexico
R. E. Carvajal
Affiliation:
Departamento de Bioquimica, Facultad de Medicina, UNAM
R. Hernandez
Affiliation:
Unidad de Investigación en Enfermedades Infecciosas y Parasitarias, Hospital de Pediatría, C.M.N.
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Summary

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Amounts of Mycobacterium tuberculosis antibodies were determined in sera from patients with either active or inactive tuberculosis and in healthy subjects by an immunoenzymatic assay in which whole BCG cells attached covalently to polystyrene disks were used as antigen. Statistically significant differences (P < 0·005) were found both between the active and inactive tuberculosis groups and between the active group and healthy controls. No significant differences were found between the inactive group and controls. Since this procedure is efficient (91%) and can be used in areas which lack laboratory equipment, it appears promising for individual serodiagnosis and for epidemiological surveys.

Type
Research Article
Information
Journal of Hygiene , Volume 97 , Issue 3 , December 1986 , pp. 483 - 487
Copyright
Copyright © Cambridge University Press 1986

References

REFERENCES

Galen, R. S. & Gambino, R. S. (1975). Beyond Normality: The Predictive Value and Efficiency of Medical Diagnoses. New York: John Wiley & Sons, Inc.Google Scholar
Grange, J. M., Gibson, J. & Nassau, E. (1980). Enzyme-linked immunosorbent assay (ELISA): a study of antibodies to Mycobacterium tuberculosis in the IgG, IgA and IgM classes in tuberculosis, sarcoidosis and Crohn's disease. Tubercle 61, 145–152.CrossRefGoogle Scholar
Hernáandez, R., Muñnoz, O. & Guiscafre, H. (1984). Sensitive enzyme immunoassay for early diagnosis of tuberculous meningitis. Journal of Clinical Microbiology 20, 533–535.CrossRefGoogle Scholar
Kalish, S. B., Radin, R. C., Phair, J. P., Levitz, D., Zeiss, C. R. & Metzger, E. (1983). Use of enzyme-linked immunoabsorbent assay technique in the differential diagnosis of active pulmonary tuberculosis in humans. The Journal of Infectious Diseases 147, 523–529.CrossRefGoogle Scholar
Káardjito, T. & Grange, J. M. (1980). Immunological and clinical features of smear-positive pulmonary tuberculosis in East-Java. Tubercle 61, 231–238.CrossRefGoogle Scholar
Nakane, P. (1974). Peroxidase-labeled antibody. A new method of conjugation. The Journal of Histochemistry and Cytochemistry 22, 1084–1091.CrossRefGoogle ScholarPubMed
Nassau, E., Parsons, E. R. & Johnson, G. D. (1976). The detection of antibodies to Mycobacterium tuberculosis by microplate enzyme-linked immunoabsorbent assay (ELISA). Tubercle 57, 67–70.CrossRefGoogle Scholar
Nicholls, A. C. (1975). A serodiagnostic test for tuberculosis. Journal of Clinical Pathology 28 850–853.CrossRefGoogle ScholarPubMed
Ruitenberg, E. J., Steerenberg, P. A., Brosi, B. J. M. & Buys, J. (1976). Evaluation of the reability of immunoenzymatic techniques for the serodiagnosis in Trichinella spiralis infections. In Immunoenzimatic Techniques (ed. Feldmann, G., Duret, P., Bigrion, J. and Avrameas, S), pp. 149–165. Amsterdam: Elsevier/North Holland.Google Scholar
Tandon, A., Saxena, R. P. & Saxena, K. C. (1980). Diagnostic potencialities of enzyme-linked immunosorbent assay in tuberculosis using purified tuberculin antigen. Tubercle 61, 87–89.CrossRefGoogle Scholar
Winters, W. D. & Cox, R. A. (1981). Serodiagnosis of tuberculosis by radioimmunoassay. American Review of Respiratory Diseases 124, 582–585.Google ScholarPubMed