Skip to main content
×
Home
    • Aa
    • Aa

Epidemiology of Escherichia coli serogroups O26, O103, O111 and O145 in very young (‘bobby’) calves in the North Island, New Zealand

  • H. IRSHAD (a1) (a2), A. L. COOKSON (a1) (a3), D. J. PRATTLEY (a1), J. MARSHALL (a1) and N. P. FRENCH (a1) (a4)...
Summary
SUMMARY

The prevalence and spatial distribution of Escherichia coli serogroups O26, O103, O111 and O145 in calves <7 days old in New Zealand and their relationship with serum IgG, weight and sex was determined by collecting recto-anal mucosal swabs (RAMS) (n = 299) and blood samples (n = 299) from two slaughter plants in the North Island. Real-time PCR of RAMS enrichment cultures revealed that 134/299 samples were positive for O26, 68/299 for O103 and 47/299 for O145, but none were positive for O111. Processing of positive enrichment cultures resulted in 49 O26, four O103 and five O145 isolates. Using multiplex PCR 25/49 (51%) O26 isolates were positive for stx1, eae, ehxA, 17/49 (34·7%) for eae, ehxA and 7/49 (14·2%) for eae only. All O103 and O145 isolates were positive for eae, ehxA only. O26 isolates were grouped into four clusters (>70% similarity) using pulsed field gel electrophoresis. Mapping of the farms showed the presence of farms positive for O26, O103 and O145 in three important dairy producing regions of the North Island. Calves positive for O103 were more likely to be positive for O26 and vice versa (P = 0·04). Similarly, calves positive for O145 were more likely to be positive for O103 and vice versa (P = 0·03). This study demonstrates that non-O157 E. coli serogroups of public health and economic importance containing clinically relevant virulence factors are present in calves in the North Island of New Zealand.

Copyright
Corresponding author
*Author for correspondence: H. Irshad, Animal Health Programme, Animal Sciences Institute, National Agricultural Research Centre, Park Road, Islamabad, Pakistan. (Email: hamidirshad@hotmail.com)
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

1. KA Bettelheim . The non-O157 Shiga-toxigenic (Verocytotoxigenic) Escherichia coli; under-rated pathogens. Critical Reviews in Microbiology 2007; 33: 6787.

2. MA Karmali , V Gannon , JM Sargeant . Verocytotoxin-producing Escherichia coli (VTEC). Veterinary Microbiology 2010; 140: 360370.

4. JG Mainil , G Daube . Verotoxigenic Escherichia coli from animals, humans and foods: who's who? Journal of Applied Microbiology 2005; 98: 13321344.

6. CC Conrad , Further development of sample preparation and detection methods for O157 and the top 6 non-O157 STEC serogroups in cattle feces. Journal of Microbiological Methods 2014; 105: 2230.

9. DD Hancock , Multiple sources of Escherichia coli O157 in feedlots and dairy farms in the northwestern USA. Preventive Veterinary Medicine 1998; 35: 1119.

10. RE Luna-Gierke , Multiple-aetiology enteric infections involving non-O157 Shiga toxin-producing Escherichia coli-FoodNet, 2001–2010. Zoonoses Public Health 2014; 61: 492498.

11. S Buncic , SM Avery . Escherichia coli O157:H7 in healthy dairy cows. New Zealand Veterinary Journal 1997; 45: 4548.

12. AL Cookson , SCS Taylor , GT Attwood . The prevalence of Shiga toxin-producing Escherichia coli in cattle and sheep in the lower North Island, New Zealand. New Zealand Veterinary Journal 2006; 54: 2833.

13. AL Cookson , Serotypes and analysis of distribution of Shiga toxin-producing Escherichia coli from cattle and sheep in the lower North Island, New Zealand. New Zealand Veterinary Journal 2006; 54: 7884.

14. P Jaros , A prospective case–control and molecular epidemiological study of human cases of Shiga toxin-producing Escherichia coli in New Zealand. BMC Infectious Diseases 2013; 13: 1450.

15. P Jaros , Nationwide prevalence and risk factors for faecal carriage of Escherichia coli O157 and O26 in very young calves and adult cattle at slaughter in New Zealand. Epidemiology and Infection 2016; 144: 17361747.

19. H Irshad , Epidemiology of Shiga toxin-producing Escherichia coli O157 in very young calves in the North Island of New Zealand. New Zealand Veterinary Journal 2012; 60: 2126.

20. S Perelle , Detection by 5 ‘-nuclease PCR of Shiga-toxin producing Escherichia coli O26, O55, O91, O103, O111, O113, O145 and O157 : H7, associated with the world's most frequent clinical cases. Molecular and Cellular Probes 2004; 18: 185192.

21. PM Fratamico , DNA sequence of the Escherichia coli O103 O antigen gene cluster and detection of enterohemorrhagic E. coli O103 by PCR amplification of the wzx and wzy genes. Canadian Journal of Microbiology 2005; 51: 515522.

22. PM Fratamico , PCR detection of enterohemorrhagic Escherichia coli O145 in food by targeting genes in the E. coli O145 O-antigen gene cluster and the Shiga toxin 1 and Shiga toxin 2 genes. Foodborne Pathogens and Disease 2009; 6: 605611.

23. H Irshad , Diversity and relatedness of Shiga toxin-producing Escherichia coli and Campylobacter jejuni between farms in a dairy catchment. Epidemiology and Infection 2016; 144: 14061417.

25. VK Sharma , EA Dean-Nystrom . Detection of enterohemorrhagic Escherichia coli O157:H7 by using a multiplex real-time PCR assay for genes encoding intimin and Shiga toxins. Veterinary Microbiology 2003; 93: 247260.

28. BD Ripley . The second-order analysis of stationary point processes. Journal of Applied Probability 1976; 13: 255266.

30. VK Sharma . Detection and quantitation of enterohemorrhagic Escherichia coli O157, O111, and O26 in beef and bovine feces by real-time polymerase chain reaction. Journal of Food Protection 2002; 65: 13711380.

31. MC Pearce , Prevalence and virulence factors of Escherichia coli serogroups O26, O103, O111, and O145 shed by cattle in Scotland. Appliedand Environmental Microbiology 2006; 72(1): 653659.

32. KM O'Reilly , Associations between the presence of virulence determinants and the epidemiology and ecology of zoonotic Escherichia coli . Applied and Environmental Microbiology 2010; 76: 81108116.

33. MC Pearce , Temporal shedding patterns and virulence factors of Escherichia coli serogroups O26, O103, O111, O145, and O157 in a cohort of beef calves and their dams. Applied and Environmental Microbiology 2004; 70: 17081716.

34. H Schmidt , Non-O157:H7 pathogenic Shiga toxin-producing Escherichia coli: phenotypic and genetic profiling of virulence traits and evidence for clonality. Journal of Infectious Diseases 1999; 179: 115123.

36. M Bielaszewska , Shiga toxin-negative attaching and effacing Escherichia coli: distinct clinical associations with bacterial phylogeny and virulence traits and inferred in-host pathogen evolution. Clinical Infectious Diseases 2008; 47: 208217.

37. RT Hernandes , An overview of atypical enteropathogenic Escherichia coli . FEMS Microbiology Letters 2009; 297: 137149.

38. KN Norman , Association of nucleotide polymorphisms within the O-antigen gene cluster of Escherichia coli O26, O45, O103, O111, O121, and O145 with serogroups and genetic subtypes. Applied and Environmental Microbiology 2012; 78: 66896703.

39. AL Cookson , Intimin subtyping of Escherichia coli: concomitant carriage of multiple intimin subtypes from forage-fed cattle and sheep. FEMS Microbiology Letters 2007; 272: 163171. doi: 10.1111/j.1574–6968.2007.00755.x.

40. E Hofer , Application of a Real-Time PCR-based system for monitoring of O26, O103, O111, O145 and O157 Shiga toxin-producing Escherichia coli in cattle at slaughter. Zoonoses and Public Health 2012; 59: 408415.

41. JT Brooks , Non-O157 Shiga toxin-producing Escherichia coli infections in the United States, 1983–2002. Journal of Infectious Diseases 2005; 192: 14221429.

43. JA Crump , DR Murdoch , MG Baker . Emerging infectious diseases in an island ecosystem: the New Zealand perspective. Emerging Infectious Diseases 2001; 7: 767772.

44. J Benschop , Informing surveillance programmes by investigating spatial dependency of subclinical Salmonella infection. Epidemiology and Infection 2009; 137: 13481359.

45. SE Fenton , Spatial and spatio-temporal analysis of Salmonella infection in dairy herds in England and Wales. Epidemiology and Infection 2009; 137: 847857.

46. G Bretschneider , EM Berberov , RA Moxley . Reduced intestinal colonization of adult beef cattle by Escherichia coli O157: H7 tir deletion and nalidixic-acid-resistant mutants lacking flagellar expression. Veterinary Microbiology 2007; 125: 381386.

47. F Dziva , Vaccination of calves with EspA, a key colonisation factor of Escherichia coli O157:H7, induces antigen-specific humoral responses but does not confer protection against intestinal colonisation. Veterinary Microbiology 2007; 123: 254261.

48. PM van Diemen , Subunit vaccines based on intimin and Efa-1 polypeptides induce humoral immunity in cattle but do not protect against intestinal colonisation by enterohaemorrhagic Escherichia coli O157:H7 or O26:H. Veterinary Immunology and Immunopathology 2007; 116: 4758.

49. AA Potter , Decreased shedding of Escherichia coli O157:H7 by cattle following vaccination with type III secreted proteins. Vaccine 2004; 22: 362369.

50. H Rugbjerg , EM Nielsen , JS Andersen . Risk factors associated with faecal shedding of verocytotoxin-producing Escherichia coli O157 in eight known-infected Danish dairy herds. Preventive Veterinary Medicine 2003; 58: 101113.

51. DA Widiasih , Duration and magnitude of faecal shedding of Shiga toxin-producing Escherichia coli from naturally infected cattle. Epidemiology and Infection 2004; 132: 6775.

52. DG Riley , Escherichia coli O157:H7 prevalence in fecal samples of cattle from a south-eastern beef cow-calf herd. Journal of Food Protection 2003; 66: 17781782.

53. K Shinagawa , Frequency of Shiga toxin-producing Escherichia coli in cattle at a breeding farm and at a slaughterhouse in Japan. Veterinary Microbiology 2000; 76: 305309.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Full text views

Total number of HTML views: 12
Total number of PDF views: 46 *
Loading metrics...

Abstract views

Total abstract views: 164 *
Loading metrics...

* Views captured on Cambridge Core between 7th March 2017 - 19th September 2017. This data will be updated every 24 hours.