Hostname: page-component-848d4c4894-5nwft Total loading time: 0 Render date: 2024-05-18T08:37:37.271Z Has data issue: false hasContentIssue false
  • English
  • Français

Further studies of incubation at 44° C. as a test for ‘faecal coli’

Published online by Cambridge University Press:  15 May 2009

H. P. Sherwood  and
L. F. L. Clegg
H. P. Sherwood
Affiliation:
Ministry of Agriculture and FisheriesFisheries Experiment Station, Conway, Caernarvonshire
L. F. L. Clegg
Affiliation:
Ministry of Agriculture and FisheriesFisheries Experiment Station, Conway, Caernarvonshire
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. The authors concluded in a previous paper that the most reliable single test for the detection of Bact. coli was incubation in MacConkey's broth at 44° C, such a test for Bact. coli alone being necessary in shellfish because of occasional multiplication of other coliforms in purified mussels. The present investigation is an attempt to verify this finding on a broader basis.

2. A description is given of the isolation of 1600 cultures of coliform organisms from polluted mussels, sewage, and faeces (human, cow, sheep and sea birds). Their classification according to ‘IMViC’ reactions and the production of gas in MacConkey's broth at temperatures of 37, 41, 44, 45 and 46° C. is given.

3. Of the + + − − type, 1036 out of 1065 cultures (97·3%) were found to produce gas in MacConkey's broth at 44° C. A balance is noted between the + + − − type which does not produce gas at 44° C. (irregular I) and the two irregular types which do so (irregular II and irregular VI). Such a balance was reported by Bardsley (1938).

4. From the cultures examined it is confirmed that incubation at 44° C. is the most suitable temperature for use with MacConkey's broth for permitting the maximum number of Bact. coli to produce gas while inhibiting the maximum number of other coliforms.

Type
Research Article
Information
Journal of Hygiene , Volume 42 , Issue 1 , January 1942 , pp. 45 - 54
Copyright
Copyright © Cambridge University Press 1942

References

REFERENCES

Banerjea, R., & Sen, A. K., (1940). Study of the Eijkman test and modifications as given by coliform, organisms isolated from human faeces. Ind. J. med. Res. 28, 315.Google Scholar
Bardsley, D. A., (1938). A comparison of two methods for assessing the number of different types of conform organisms in water. J. Hyg., Camb., 38, 309.CrossRefGoogle Scholar
Clegg, L. F. L., (1941). The bacteriological examination of water samples with reference to direct and secondary incubation at 44° C. J. Path. Bact. 53, 51.CrossRefGoogle Scholar
Clegg, L. F. L., & Sherwood, H. P., (1939). Incubation at 44° C. as a test for faecal coli. J. Hyg., Camb., 39, 361.Google Scholar
Dodgson, R. W., (1936). Shellfish and the public health. Brit. med. J. 2, 169.CrossRefGoogle Scholar
Dodgson, R. W., (1937). Shellfish and the public health. Public Health, 06.Google Scholar
Dodgson, R. W., (1938). Shellfish and the public health. Proc. R. Soc. Med. 31, 925.Google ScholarPubMed
Ferramola, R., (1940). Wilson method for the bacteriological examination of waiter. Amer. J. Publ. Hlth, 30, 1083.CrossRefGoogle Scholar
Ferramola, R., & Monteverde, J. J., (1938). Incubation at 44° C. as a means of proving the presence of B. coli. Bol. Obras. Sanit., Buenos Aires, 3, 265.Google Scholar
Ferramola, R., & Monteverde, J. J., (1939). Incubation at 44° C. as a means of indicating the presence of B. coli. Bol. Obras. Sanit, Buenos Aires, 4, 248.Google Scholar
Hajna, A. A., & Perry, C. A., (1939). Optimum temperature for differentiation of Esch. coli from other coliform bacteria. J. Bact. 38, 275.CrossRefGoogle Scholar
Ministry of Health (1939). Rep. Publ. Hlth Med. Subj., Lond., no. 71, rev. ed.Google Scholar
Parr, L. W., (1938). The occurrence and succession of coliform organisms in human faeces. Amer. J. Hyg. 27, 67.Google Scholar
Raven, C., Peden, D., & Wright, H. D., (1940). The bacteriological examination of water supplies. J. Path. Bact. 50, 287.CrossRefGoogle Scholar
Taylor, C. B., (1941). The ecology and significance of different types of colifonn bacteria found in water. J. Hyg., Camb., 41, 23.Google Scholar
Wilson, G. S., Twig, R. S., Wright, R., C, Hendry, C. B., Cowell, M. P., & Maier, I., (1935). The bacteriological grading of milk. Spec: Rep. Ser. Med. Res. Coun., Lond., no. 206. London: H.M. Stationery Office.Google Scholar