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Genotyping of Pseudomonas aeruginosa sputum and stool isolates from cystic fibrosis patients: evidence for intestinal colonization and spreading into toilets

Published online by Cambridge University Press:  15 May 2009

G. Döring ,
H. Bareth ,
A. Gairing ,
C. Wolz  and
K. Botzenhart
G. Döring
Affiliation:
Department of General and Environmental Hygiene, Hygiene Institute, University of Tübingen, Tübingen, Federal Republic of Germany
H. Bareth
Affiliation:
Department of General and Environmental Hygiene, Hygiene Institute, University of Tübingen, Tübingen, Federal Republic of Germany
A. Gairing
Affiliation:
Department of General and Environmental Hygiene, Hygiene Institute, University of Tübingen, Tübingen, Federal Republic of Germany
C. Wolz
Affiliation:
Department of General and Environmental Hygiene, Hygiene Institute, University of Tübingen, Tübingen, Federal Republic of Germany
K. Botzenhart
Affiliation:
Department of General and Environmental Hygiene, Hygiene Institute, University of Tübingen, Tübingen, Federal Republic of Germany
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Three hundred and fifty-eight stool and 131 sputum specimens from 40 cystic fibrosis (CF) patients and 100 toilet sinks were investigated for occurrence of Pseudomonas aeruginosa; 67% (21/31) of the patients with chronic P. aeruginosa lung infections carried the organism repeatedly in the stool but the organism was found only once in the stools of nine uninfected patients. P. aeruginosa stool carriage was correlated to high P. aeruginosa numbers in patients' sputa. Typing of P. aeruginosa with a DNA probe showed identity of sputum and stool strains. Seven patients repeatedly carried additional stool strains, not found in the sputum, suggesting intestinal colonization. No differences were seen in the clinical state of patients with P. aeruginosa-negative stool samples and patients with positive stool samples. Toilets in households of P. aeruginosa-infected CF patients were significantly more often contaminated with P. aeruginosa (42%) than toilets in households of non-infected CF patients (20%; P < 0·03). The study shows that P. aeruginosa-infected CF patients may harbour the organisms also in the intestinal tract, and may spread the bacteria into toilets.

Type
Research Article
Information
Epidemiology & Infection , Volume 103 , Issue 3 , December 1989 , pp. 555 - 564
Copyright
Copyright © Cambridge University Press 1989

References

REFERENCES

1.Høiby, N.Microbiology of lung infections in cystic fibrosis patients. Acta Paediatr Scand Suppl 1982; 301:33–54.Google Scholar
2.Speert, DP, Campbell, ME.Hospital epidemiology of Pseudomonas aeruginosa from patients with cystic fibrosis. J Hosp Infect 1987; 9: 11–21.CrossRefGoogle ScholarPubMed
3.Kessner, DM, Lepper, MH.Epidemiologic studies of gram-negative bacilli in the hospital and community. Am J Epidem 1967; 85:45–60.Google Scholar
4.Roy, CC, Delage, G, Fontaine, A, Robitaille, L, Chartraud, L, Weber, A, Morin, CL.The fecal microflora and bile acids in children with cystic fibrosis. Am J Clin Nutr 1979; 12: 2404–9.Google Scholar
5.Kelly, NM, Falkiner, FR, Keane, CT.Acetamid broth for isolation of Pseudomonas aeruginosa from patients with cystic fibrosis. J Clin Microbiol 1983; 17: 159.CrossRefGoogle ScholarPubMed
6.Agnarsson, U, Glass, S, Govan, JRW.Fecal isolation of Pseudomonas aeruginosa from patients with cystic fibrosis. J Clin Microbiol 1989; 27: 96–8.Google Scholar
7.Wolz, C, Kiosz, G, Ogle, JW, Vasil, ML, Schaad, U, Botzenhart, K, Döring, G.Pseudomonas aeruginosa cross-colonization and persistence in patients with cystic fibrosis. Use of a DNA probe. Epidemiol Infect 1989; 102: 205–14.Google Scholar
8.Gerba, CP, Wallis, C, Melnick, JL.Microbial hazards of household toilets: droplet production and the fate of residual organisms. App Microbiol 1975; 30: 229–37.Google Scholar
9.Darlow, HM, Bale, WR.Infective hazards of water-closets. Lancet 1959; i: 1196–200.Google Scholar
10.Wood, RE, Boat, TF, Doershuk, CF.Cystic fibrosis: state of the art. Am Rev Respir Dis 1976; 113: 833–78.Google Scholar
11.Shooter, RA, Walker, KA, Williams, VR, Horgan, GM, Parker, MT, Asheshov, EH, Bullimore, JF.Faecal carriage of Pseudomonas aeruginosa in hospital patients. Possible spread from patient to patient. Lancet 1966; 2: 1314.Google Scholar
12.Tancrède, CH, Andremont, AO.Bacterial translocation and gram-negative bacteremia in patients with hematological malignancies. J Infect Dis 1985; 152: 99–103.CrossRefGoogle ScholarPubMed
13.Ogle, JW, Janda, JM, Woods, DE, Vasil, ML.Characterization and use of a DNA probe as an epidemiological marker for Pseudomonas aeruginosa. J Infect Dis 1987; 155: 119–26.Google Scholar
14.Johnson, JL. Genetic characterization. In: Gerhardt, P, ed. Manual of methods for general bacteriology. Washington, DC: American Society for Microbiology, 1981; 450–72.Google Scholar
15.Southern, E.Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 1975; 98: 503–17.Google Scholar
16.Vasil, M, Chamberlain, C, Grant, C.Molecular studies of Pseudomonas exotoxin A gene. Infect Immun 1986; 52: 538–48.Google Scholar
17.Maniatis, T, Fritsch, EF, Sambrock, J.Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbour Laboratory, 1982.Google Scholar
18.Vogt, D.Über den gegenwärtigen Stand der Akzeleration in Bayern. Arch Kinderheilk 1959; 159: 141–5.Google Scholar
19.Levison, ME. Factors influencing colonization of the gastrointestinal tract with Pseudomonas aeruginosa. In: Young, VM, ed. Pseudomonas aeruginosa: Ecological Aspects and Patient Colonization. New York: Raven Press, 1977.Google Scholar
20.Hentges, DJ, Stein, AJ, Casey, SW, Que, JU.Protective role of intestinal flora against infection with Pseudomonas aeruginosa in mice: influence of antibiotics on colonization resistance. Infect Immun 1985; 47: 118–22.Google Scholar
21.Lanyi, B, Gregacs, M, Adam, MM.Incidence of Pseudomonas aeruginosa serotypes in water and human faeces. Acta Microbiol Hung 1966; 13: 319–26.Google Scholar
22.Linde, K, Kittlick, M.Zum Nachweis von Bacterium pyocyaneum in menschlichem Untersuchungsmaterial. Arch Hyg 1963; 146: 126–38.Google Scholar
23.Remington, JS, Schimpff, SC.Please don't eat the salads. New Engl J Med 1981; 304: 433–5.Google Scholar
24.Bruce, MC, Poncz, L, Klinger, JD, Stern, RC, Tomashefski, JF Jr, Dearborn, DG.Biochemical and pathological evidence for proteolytic destruction of lung connective tissue in cystic fibrosis. Am Rev Respir Dis 1985; 132: 529–35.Google Scholar
25.Buck, AC, Cooke, EM.The fate of ingested Pseudomonas aeruginosa in normal persons. J Med Microbiol 1969; 2: 521–5.Google Scholar
26.Lloyd-Still, JD. Growth, nutrition and gastrointestinal problems. In: Lloyd-Still, JD, ed. Testbook of Cystic Fibrosis. Bristol: John Wright, 1983; 223–67.Google Scholar
27.Moss, RB. Immunology of cystic fibrosis: immunity, immunodeficiency, and hypersensitivity. In: Lloyd-Still, JD, ed. Textbook of Cystic Fibrosis. Bristol: John Wright. 1983; 109–52.Google Scholar
28.Vander Wauven, C, Piérard, A, Kley-Raymann, M, Haas, D.Pseudomonas aeruginosa mutants affected in anaerobic growth on arginine: evidence for a four-gene cluster encoding the arginine deiminase pathway. J Bacteriol 1984; 160: 928–32.Google Scholar