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Immunofluorescent study of the spore antigens of proteolyticstrains of Clostridium botulinum

Published online by Cambridge University Press:  15 May 2009

T. J. T. Princewill
T. J. T. Princewill
Affiliation:
Department of Microbiology, Agricultural Science Building, The University, Leeds, LS2 9JT, England
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By means of the spore fluorescent antibody technique, 31 strains of Clostridium botulinum types A (18 strains), B (10 strains) and F (3 strains) were found to belong to the same homogeneous group irrespective of their toxigenic types. Some strains of this species also cross-reacted with certain strains of Clostridium sporogenes types I, II and III and Clostridium histolyticum type II. By spore antigenic analysis it was found that Clostridium parabotulinum contained two components designated L and M, the former describing species specificity; the latter was the cross-reacting component shared by some strains of Clostridium sporogenes and Clostridium histolyticum. Following this, a scheme showing the distribution of spore antigenic components among various species of Clostridium was given.

Type
Research Article
Information
Journal of Hygiene , Volume 83 , Issue 1 , August 1979 , pp. 1 - 9
Copyright
Copyright © Cambridge University Press 1979

References

Bengtson, I. A. (1924). Studies on organisms concerned as causative factors in botulism. U.S. Public Health Service, Hygiene Laboratory Bulletin, No. 136.Google Scholar
Lynt, R. K. Jr., solomon, H. M. & Kautter, D. A. (1971). Immuno-fluorescence among strains of Clostridium botulinum and other clostridia by direct and indirect methods. Journal of Food Science 36, 594.CrossRefGoogle Scholar
Lynt, R. K. JR., Solomon, H. M. & Kautter, D. A. (1972). Specificity of somatic antisera for detection of Clostridium botulinum by immunofluorescence. Spore 5, 344.Google Scholar
Mandia, J. W. (1951). Serological group II of the proteolytic clostridia. Journal of Immunology 67, 49.CrossRefGoogle ScholarPubMed
Mandia, J. W. (1955). The position of Clostridium tetani within the serological schema for the proteolytic clostridia. Journal of Infectious Diseases 97, 66.CrossRefGoogle ScholarPubMed
Meisel, H. & Rymkiewicz, D. (1959). Antygeny zarodnikowe w klasyfikacji proteolitycznych clostridia. Medycyna Doswiadczalna i Mikrobiologia 11, 1.Google Scholar
Princewill, T. J. T. (1978). Differences in colony morphology and carbohydrate fermentation of Clostridium sporogenes. Journal of General Microbiology 108, 315.CrossRefGoogle Scholar
Princewill, T. J. T. (1979a). Spore antigens of Clostridium sporogenes. Journal of Medical Microbiology 12, 29.CrossRefGoogle ScholarPubMed
Princewill, T. J. T. (1979b). Spore antigens for the classification of some clostridia. Journal of Medical Microbiology (in the Press).Google ScholarPubMed
Solomon, H. M., Lynt, R. K. JR., Kautter, D. A. & Lilly, T. JR. (1971). Antigenic relationships among the proteolytic and non-proteolytic strains of Clostridium botulinum. Applied Microbiology 21, 295.CrossRefGoogle Scholar
Walker, P. D. & Batty, I. (1964). Further studies in the genus Clostridium. II. A rapid method for differentiating Clostridium botulinum types A, B and F, types C and D and type E. Journal of Applied Bacteriology 27, 140.CrossRefGoogle Scholar