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The incorporation of radioactive phosphorus in the influenza virus and its distribution in serologically active virus fractions

Published online by Cambridge University Press:  15 May 2009

L. Hoyle ,
B. Jolles  and
R. G. Mitchell
L. Hoyle
Affiliation:
Public Health Laboratory, Northampton, and the Radiotherapy Department, General Hospital, Northampton
B. Jolles
Affiliation:
Public Health Laboratory, Northampton, and the Radiotherapy Department, General Hospital, Northampton
R. G. Mitchell
Affiliation:
Public Health Laboratory, Northampton, and the Radiotherapy Department, General Hospital, Northampton
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1. When the D.S.P. strain of influenza virus A is grown in eggs into which 100 μc. of radioactive inorganic phosphate has been introduced the virus incorporates 32P into its structure.

2. Some 20–25% of the virus 32P is found in the virus phospholipid; the remainder is combined with the virus protein and is probably present in the virus nucleic acid.

3. When the virus is disintegrated by ether treatment with the liberation of separate red-cell agglutinating and complement-fixing ‘soluble antigen’ particles the non-lipid 32P is found to be associated with the soluble antigen fraction and not with the haemagglutinin.

4. It is suggested that the complement-fixing soluble antigen is a nucleoprotein while the haemagglutinin is a phosphorus-free protein.

Type
Research Article
Information
Journal of Hygiene , Volume 52 , Issue 1 , March 1954 , pp. 119 - 127
Copyright
Copyright © Cambridge University Press 1954

References

REFERENCES

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