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    Coenye, Tom Vandamme, Peter and LiPuma, John J. 2002. Infection byRalstoniaSpecies in Cystic Fibrosis Patients: Identification ofR. pickettiiandR. mannitolilyticaby Polymerase Chain Reaction. Emerging Infectious Diseases, Vol. 8, Issue. 7, p. 692.

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Investigation of a pseudo-outbreak of ‘Pseudomonas thomasii’ in a special-care baby unit by numerical analysis of SDS–PAGE protein patterns

  • M. Costas (a1), B. Holmes (a1), L. L. Sloss (a1) and S. Heard (a2)
  • DOI:
  • Published online: 01 May 2009

Forty-two cultures of pseudomonas comprising 28 clinical isolates from a pseudo-outbreak on a Special-Care Baby Unit and 14 reference strains, including 9 type strains, of various Pseudomonas species, were characterized by one-dimensional sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) of whole-cell proteins. The protein patterns were highly reproducible and were used as the basis for a numerical analysis which divided the strains into 9 phenons. Two of the 28 clinical isolates were identified by biochemical tests as P. pickettii and their identification was confirmed by SDS–PAGE as they fell in the same phenon as the type strain of the species. The remaining 26 isolates, which could not be identified on phenotypic tests, fell in the same phenon as three reference strains of ‘P. thomasii’. The protein patterns provided the first clear evidence that P. pickettii and ‘P. thomasii’ were separate taxa and that the ‘outbreak’ was polymicrobial in origin, in line with the probable aqueous source of contamination. We conclude that high-resolution SDS–PAGE of proteins provides an effective method of identifying and differentiating pseudomonads, especially where this cannot be done adequately using conventional biochemical tests.

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5.RM Baird , KM Elhag , EJ Shaw . Pseudomonas thomasii in a hospital distilled-water supply. J Med Microbiol 1976; 9, 493–5.

7.A King , B Holmes , I Phillips , SP Lapage . A taxonomic study of clinical isolates of Pseudomonas pickettii, ‘P. thomasii’ and ‘group IVd’ bacteria. J Gen Microbiol 1979; 114: 137–47.

12.K Kersters . Numerical methods in the classification of bacteria by protein electrophoresis. In: M Goodfellow , D Jones , FG Priest , eds. Computer assisted bacterial systematics. London: Academic Press. 1985: 337–68.

15.M Costas , B Holmes , AC Wood , SLW On . Numerical analysis of electrophoretic protein patterns of Providencia rettgeri strains from human faeces, urine and other specimens. J Appl Bacteriol 1989; 67: 441–52.

16.B Holmes , M Costas , AC Wood . Numerical analysis of electrophoretic protein patterns of Group EF-4 bacteria, predominantly from dog-bite wounds of humans. J Appl Bacteriol 1990; 68: 8191.

17.S Heard , S Lawrence , B Holmes , M Costas . A pseudo-outbreak of Pseudomonas on a special care baby unit. J Hosp Infect 1990. In press.

20.M Costas , B Holmes , LL Sloss . Numerical analysis of electrophoretic protein patterns of Providencia rustigianii strains from human diarrhoea and other sources. J Appl Bacteriol 1987; 63: 319328.

23.PHA Sneath , R Johnson . The influence on numerical taxonomic similarities of errors in microbiological tests. J Gen Microbiol 1972; 72: 377–91.

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Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
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