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Isolates of Salmonella enterica Enteritidis PT4 with enhanced heat and acid tolerance are more virulent in mice and more invasive in chickens

  • T. J. Humphrey (a1), A. Williams (a2), K. McAlpine (a1), M. S. Lever (a2), J. Guard-Petter (a3) and J. M. Cox (a4)...

Summary

Two Enteritidis PT4 isolates which differed in inherent tolerance to heat, acid, H2O2 and the ability to survive on surfaces were used to infect mice, day-old chicks or laying hens. The acid-, heat-, H2O2- and surface-tolerant isolate was more virulent in mice and more invasive in laying hens, particularly in reproductive tissue. However, no significant differences were observed in behaviour in chicks. Both PT4 isolates were able to infect chicks housed in the same room as infected birds, although the heat-tolerant isolate survived significantly better than the heat-sensitive one in aerosols.

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Copyright

References

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1.Advisory Committee on the Microbiological Safety of Food. Salmonella in eggs. London: HMSO, 1993.
2.Humphrey, TJ, Slater, E, McAlpine, K, Rowbury, RJ, Gilbert, RJ.Salmonella enteritidis PT4 isolates more tolerant of heat, acid, or hydrogen peroxide also survive longer on surfaces. Appl Environ Microbiol 1995; 61: 3161–4.
3.Petter, JG.Detection of two smooth colony phenotypes in a Salmonella enteritidis isolate which vary in their ability to contaminate eggs. Appl. Environ Microbiol 1993; 59: 2884–90.
4.Guard-Petter, J, Lakshmi, B, Carlson, R, Ingram, K.Characterization of lipopolysaccharide heterogeneity in Salmonella enteritidis by an improved gel electro-phoresis method. Appl Environ Microbiol 1995; 61: 2845–51.
5.Hinton, M, Pearson, GR, Threlfall, EJ, Rowe, B.Experimental Salmonella enteritidis infection in chicks. Vet Rec 1989; 124: 223.
6.Hinton, M, Threlfall, EJ, Rowe, B.The invasiveness of different strains of Salmonella enteritidis phage type 4 for young chickens. FEMS Microbiol Lett 1990; 70: 193–6.
7.Hinton, M, Threlfall, E, Rowe, B.The invasive potential of Salmonella enteritidis phage types for young chickens. Lett Appl Microbiol 1990; 10: 237–9.
8.Cox, JM, Woolcock, JB.Lipopolysaccharide expression and virulence in mice of Australian isolates of Salmonella enteritis. Lett Appl Microbiol 1994; 19: 95–8.
9.Chart, H, Rowe, B.Antibodies to lipopolysaccharide and outer membrane proteins of Salmonella enteritidis PT4 are not involved in protection from experimental infection. FEMS Microbiol Lett 1991; 84: 345–50.
10.Baskerville, A, Humphrey, TJ, Fitzgeorge, RB, et al. Airborne infection of laying hens with Salmonella enteritidis phage type 4. Vet Rec 1992; 130: 395–8.
11.Humphrey, TJ, Baskerville, A, Chart, H, Rowe, B, Whitehead, A.Infection of laying hens with Salmonella enteritidis PT4 by conjunctival challenge. Vet Rec 1992; 131: 386–8.
12.Humphrey, TJ, Whitehead, A, Gawler, AH, Henley, A, Rowe, B.Numbers of Salmonella enteritidis in the contents of naturally contaminated hens' eggs. Epidemiol Infect 1991; 106: 489–96.
13.Hambleton, P, Broster, MG, Dennis, PJ, Henstridge, R, Fitzgeorge, R, Conlan, JW.Survival of virulent Legionella pneumophila in aerosols. J Hyg 1983; 90: 451–60.
14.Padron, MN.Salmonella typhimurium penetration through the eggshell of hatching eggs. Avian Dis 1990; 34: 463–5.
15.Gast, RK, Beard, CW.Production of Salmonella enteritidis-contaminated eggs by experimentally infected hens. Avian Dis 1990; 34: 438–46.
16.Humphrey, TJ, Baskerville, A, Mawer, S, Rowe, B, Hopper, S.Salmonella enteritidis phage type 4 from the contents of intact eggs: a study involving naturally infected hens. Epidemiol Infect 1989; 103: 415–23.
17.Hoop, RK, Pospischil, A.Bacteriological, serological, histological and immuno-histochemical findings in laying hens naturally infected with Salmonella enteritidis phage type 4 infection. Vet Rec 1993; 133: 391–3.
18.Lister, SA.Salmonella enteritidis infection in broilers and broiler-breeders. Vet Rec 1988; 123: 250.
19.Keller, LH, Benson, CE, Krotec, K, Eckroade, RJ.Salmonella enteritidis colonization of the reproductive tract and forming and freshly laid eggs of chickens. Infect Immun 1995; 63: 2443–9.
20.Brake, J, Thaxton, P.Physiological changes in caged layers during a forced molt. 2. Gross changes in organs. Poult Sci 1979; 58: 707–16.
21.Humphrey, TJ, Baskerville, A, Chart, H, Rowe, B, Whitehead, B.Salmonella enteritidis PT4 infection in specific pathogen free hens: influence of infecting dose. Vet Rec 1991; 129: 482–5.
22.Bygrave, A, Gallagher, J.Transmission of Salmonella enteritidis in poultry. Vet Rec 1989; 124: 571.
23.Halavatkar, H, Barrow, PL.The role of a 54-kb plasmid in the virulence of strains of Salmonella enteritidis of phage type 4 for chicken and mice. J Med Microbiol 1993; 38: 171–6.
24.Chart, H, Threlfall, EJ, Rowe, B.Virulence studies of Salmonella enteritidis phage types. Lett Appl Microbiol 1991; 12: 188–91.
25.Cox, JM.Salmonella enteritidis: virulence factors and invasive infection in poultry. Trends Food Sci Tech 1995; 6: 407–10.
26.Collinson, SK, Emödy, L, Müller, KH, Trust, TJ, Kay, WW.Purification and characterisation of thin, aggregative fimbriae from Salmonella enteritidis. J. Bacteriol 1991; 173: 4473–81.
27.Collinson, SK, Doig, PC, Doran, JL, Clouthier, S, Trust, TJ, Kay, WW.Thin, aggregative fimbriae mediate binding of Salmonella enteritidis to fibrolectin. J Bacteriol 1993; 175: 12–8.
28.Thorns, CJ, Sojka, MG, Chasey, D.Detection of a novel fimbrial structure on the surface of Salmonella enteritidis by using monoclonal antibody. J Clin Microbiol 1990; 28: 2409–14.
29.Mekalanos, JJ.Environmental signals controlling expression of virulence determinants in bacteria. J Bacteriol 1992; 174: 17.

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