Hostname: page-component-7d684dbfc8-zgpz2 Total loading time: 0 Render date: 2023-09-30T17:46:39.688Z Has data issue: false Feature Flags: { "corePageComponentGetUserInfoFromSharedSession": true, "coreDisableEcommerce": false, "coreDisableSocialShare": false, "coreDisableEcommerceForArticlePurchase": false, "coreDisableEcommerceForBookPurchase": false, "coreDisableEcommerceForElementPurchase": false, "coreUseNewShare": true, "useRatesEcommerce": true } hasContentIssue false

Klebsiella serotyping by counter-current immunoelectrophoresis

Published online by Cambridge University Press:  15 May 2009

Judith M. Palfreyman
Affiliation:
Public Health Laboratory, Coventry
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not possible as this article does not have html content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The development of a Klebsiella serotyping method by counter-current immunoelectrophoresis (CIE) is described.

Antisera were prepared against the capsular antigens of 72 type strains and tested for the specificity and strength of their precipitin reactions with antigens from homologous and heterologous serotypes. All antisera produced strong reactions with their homologous antigen: when diluted to titre 63 were highly specific, 3 cross-reacted strongly and 6 weakly with one other antigen. Pools of antisera for screening purposes were constructed on the basis of common cross-reactions: component serotypes of each pool could be detected strongly and specifically.

The technique is simple to perform, fairly rapid, and economical in the use of antisera. Results can be read easily and quickly and the intensity of cross-reactions compared directly. The technique appears to be more specific and is less time consuming than the Quellung method, but further assessment of its efficacy in typing routine clinical cultures is necessary.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1978

References

REFERENCES

Burns, M. W. (1968). Precipitins to Klebsiella and other Enterobacteria in the serum of patients with chronic respiratory disorders. Lancet i, 383–5.CrossRefGoogle Scholar
Casewell, M. W. (1975). Titres and cross-reactions of commercial antisera for the capsular typing of Klebsiella species. Journal of Clinical Pathology 28, 33–6.CrossRefGoogle ScholarPubMed
Casewell, M. W., Dalton, M. T., Webster, M. & Phillips, T. (1977). Gentamicin-resistant Klebsiella aerogenes in a urological ward. Lancet ii, 444–6.CrossRefGoogle Scholar
Edwards, P. R. & Ewing, W. H. (1972). In Identification of Enterobacteriaceae, 3rd ed., p. 298. Minneapolis: Burgess Publishing Company.Google Scholar
Eriksen, J. (1965). Immunochemical studies on some serological cross-reactions in the Klebsiella group. 12. Serological reactions with the acidic capsular polysaccharides from Klebsiella Type 3(C) as antigen. Acta pathologica et microbiologica scandinavica 64, 522–6.CrossRefGoogle ScholarPubMed
Eriksen, J. & Henriksen, S. D. (1963). Immunochemical studies on some serological cross-reactions in the Klebsiella group. 9. Cross-reactions between Klebsiella types 11, 21 and a closely related strain. Acta pathologica et microbiologica scandinavica 58, 245–50.CrossRefGoogle Scholar
Martin, W. J., Yu, P. K. W. & Washington, J. A. (1971). Epidemiologic significance of Klebsiella pneumoniae. Mayo Clinic Proceedings 46, 785–93.Google Scholar
Montgomerie, J. Z., Doak, P. B., Taylor, D. E. M., North, J. D. K. & Martin, W. J. (1970). Klebsiella in faecal flora of renal-transplant patients. Lancet ii, 787–92.CrossRefGoogle Scholar
Moody, G. J. (1976). Methodology and applications of countercurrent-immunoelectrophoresis in microbiology. Laboratory Practice 25(9), 575–80.Google ScholarPubMed
Noriega, E. R., Liebowitz, R. E., Richmond, A. S., Rubinstein, B., Schaefler, S., Simberkoff, M. S. & Rahal, J. J. (1975). Nosocomial infection caused by gentamicin-resistant, streptomycin-sensitive Klebsiella. Journal of Infectious Diseases 131, (suppl.), S45–S50.CrossRefGoogle ScholarPubMed
Price, D. J. E. & Sleigh, J. D. (1970). Control of infection due to Klebsiella aerogenes in a neurosurgical unit by withdrawal of all antibiotics. Lancet ii, 1213–5.CrossRefGoogle Scholar
Rennie, R. P. & Duncan, I. B. R. (1974). Combined biochemical and serological typing of clinical isolates of Klebsiella. Applied Microbiology 28 (4), 534–9.Google ScholarPubMed
Richard, C. (1973). Etude antigénique et biochemique de 500 souches de Klebsiella. Annales de biologie clinique 31, 295303.Google Scholar
Riser, E., Noone, P. & Bonnet, M. L. (1976a). A new serotyping method for Klebsiella species: evaluation of the technique. Journal of Clinical Pathology 29, 305–8.CrossRefGoogle ScholarPubMed
Riser, E., Noone, P. & Poulton, T. A. (1976b). A new serotyping method for Klebsiella species: development of the technique. Journal of Clinical Pathology 29, 296304.CrossRefGoogle ScholarPubMed
Selden, R., Lee, S., Wang, W. L. L., Bennett, J. V. & Eickhoff, T. (1971). Nosocomial Klebsiella infections: intestinal colonization as a reservoir. Annals of Internal Medicine 74, 657–64.CrossRefGoogle ScholarPubMed