Skip to main content Accessibility help
×
Home
Hostname: page-component-684899dbb8-gblv7 Total loading time: 0.374 Render date: 2022-05-20T11:40:16.106Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "useRatesEcommerce": false, "useNewApi": true }

Klebsiella serotyping by counter-current immunoelectrophoresis

Published online by Cambridge University Press:  15 May 2009

Judith M. Palfreyman
Affiliation:
Public Health Laboratory, Coventry
Rights & Permissions[Opens in a new window]

Summary

HTML view is not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The development of a Klebsiella serotyping method by counter-current immunoelectrophoresis (CIE) is described.

Antisera were prepared against the capsular antigens of 72 type strains and tested for the specificity and strength of their precipitin reactions with antigens from homologous and heterologous serotypes. All antisera produced strong reactions with their homologous antigen: when diluted to titre 63 were highly specific, 3 cross-reacted strongly and 6 weakly with one other antigen. Pools of antisera for screening purposes were constructed on the basis of common cross-reactions: component serotypes of each pool could be detected strongly and specifically.

The technique is simple to perform, fairly rapid, and economical in the use of antisera. Results can be read easily and quickly and the intensity of cross-reactions compared directly. The technique appears to be more specific and is less time consuming than the Quellung method, but further assessment of its efficacy in typing routine clinical cultures is necessary.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1978

References

Burns, M. W. (1968). Precipitins to Klebsiella and other Enterobacteria in the serum of patients with chronic respiratory disorders. Lancet i, 383–5.CrossRefGoogle Scholar
Casewell, M. W. (1975). Titres and cross-reactions of commercial antisera for the capsular typing of Klebsiella species. Journal of Clinical Pathology 28, 33–6.CrossRefGoogle ScholarPubMed
Casewell, M. W., Dalton, M. T., Webster, M. & Phillips, T. (1977). Gentamicin-resistant Klebsiella aerogenes in a urological ward. Lancet ii, 444–6.CrossRefGoogle Scholar
Edwards, P. R. & Ewing, W. H. (1972). In Identification of Enterobacteriaceae, 3rd ed., p. 298. Minneapolis: Burgess Publishing Company.Google Scholar
Eriksen, J. (1965). Immunochemical studies on some serological cross-reactions in the Klebsiella group. 12. Serological reactions with the acidic capsular polysaccharides from Klebsiella Type 3(C) as antigen. Acta pathologica et microbiologica scandinavica 64, 522–6.CrossRefGoogle ScholarPubMed
Eriksen, J. & Henriksen, S. D. (1963). Immunochemical studies on some serological cross-reactions in the Klebsiella group. 9. Cross-reactions between Klebsiella types 11, 21 and a closely related strain. Acta pathologica et microbiologica scandinavica 58, 245–50.CrossRefGoogle Scholar
Martin, W. J., Yu, P. K. W. & Washington, J. A. (1971). Epidemiologic significance of Klebsiella pneumoniae. Mayo Clinic Proceedings 46, 785–93.Google Scholar
Montgomerie, J. Z., Doak, P. B., Taylor, D. E. M., North, J. D. K. & Martin, W. J. (1970). Klebsiella in faecal flora of renal-transplant patients. Lancet ii, 787–92.CrossRefGoogle Scholar
Moody, G. J. (1976). Methodology and applications of countercurrent-immunoelectrophoresis in microbiology. Laboratory Practice 25(9), 575–80.Google ScholarPubMed
Noriega, E. R., Liebowitz, R. E., Richmond, A. S., Rubinstein, B., Schaefler, S., Simberkoff, M. S. & Rahal, J. J. (1975). Nosocomial infection caused by gentamicin-resistant, streptomycin-sensitive Klebsiella. Journal of Infectious Diseases 131, (suppl.), S45–S50.CrossRefGoogle ScholarPubMed
Price, D. J. E. & Sleigh, J. D. (1970). Control of infection due to Klebsiella aerogenes in a neurosurgical unit by withdrawal of all antibiotics. Lancet ii, 1213–5.CrossRefGoogle Scholar
Rennie, R. P. & Duncan, I. B. R. (1974). Combined biochemical and serological typing of clinical isolates of Klebsiella. Applied Microbiology 28 (4), 534–9.Google ScholarPubMed
Richard, C. (1973). Etude antigénique et biochemique de 500 souches de Klebsiella. Annales de biologie clinique 31, 295303.Google Scholar
Riser, E., Noone, P. & Bonnet, M. L. (1976a). A new serotyping method for Klebsiella species: evaluation of the technique. Journal of Clinical Pathology 29, 305–8.CrossRefGoogle ScholarPubMed
Riser, E., Noone, P. & Poulton, T. A. (1976b). A new serotyping method for Klebsiella species: development of the technique. Journal of Clinical Pathology 29, 296304.CrossRefGoogle ScholarPubMed
Selden, R., Lee, S., Wang, W. L. L., Bennett, J. V. & Eickhoff, T. (1971). Nosocomial Klebsiella infections: intestinal colonization as a reservoir. Annals of Internal Medicine 74, 657–64.CrossRefGoogle ScholarPubMed
You have Access
62
Cited by

Save article to Kindle

To save this article to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Klebsiella serotyping by counter-current immunoelectrophoresis
Available formats
×

Save article to Dropbox

To save this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about saving content to Dropbox.

Klebsiella serotyping by counter-current immunoelectrophoresis
Available formats
×

Save article to Google Drive

To save this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about saving content to Google Drive.

Klebsiella serotyping by counter-current immunoelectrophoresis
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *