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Microneutralization tests for serological typing and subtyping of foot-and-mouth disease virus strains

Published online by Cambridge University Press:  15 May 2009

M. M. Rweyemamu ,
J. C. Booth ,
Morwen Head  and
T. W. F. Pay
M. M. Rweyemamu
Affiliation:
The Wellcome Foundation Limited, Wellcome Foot-and-Mouth Disease Vaccine Leboratory, Pirbright, Woking, Surrey 0U24 ONQ, U.K.
J. C. Booth
Affiliation:
The Wellcome Foundation Limited, Wellcome Foot-and-Mouth Disease Vaccine Leboratory, Pirbright, Woking, Surrey 0U24 ONQ, U.K.
Morwen Head
Affiliation:
The Wellcome Foundation Limited, Wellcome Foot-and-Mouth Disease Vaccine Leboratory, Pirbright, Woking, Surrey 0U24 ONQ, U.K.
T. W. F. Pay
Affiliation:
The Wellcome Foundation Limited, Wellcome Foot-and-Mouth Disease Vaccine Leboratory, Pirbright, Woking, Surrey 0U24 ONQ, U.K.
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Summary

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A microneutralization test for serotyping of FMD viruses is described. It is based on earlier observations by Booth, Rweyemamu & Pay (1978) that dose-response relationships in quantal microneutralizations often deviated from linearity. The typing test described therefore utilizes undiluted virus preparations. In about 90% of samples a positive typing was obtained in contrast with about 50% for the complement fixation test. The test was also found to be susceptible to minimal quantities of heterotypic viral contamination.

For strain differentiation the microneutralization test was carried out as a checkerboard test. When compared with the complement fixation test it was found to be more specific. The necessity to utilize virus-neutralization test systems for comparing (FMD) virus strains particularly for the purpose of vaccine selection is emphasized. The two dimensional microneutralization test has been applied to a study of comparing FMDV vaccine strains for Europe, South America, the Middle East and East Africa.

Type
Research Article
Information
Journal of Hygiene , Volume 81 , Issue 1 , August 1978 , pp. 107 - 123
Copyright
Copyright © Cambridge University Press 1978

References

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