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Molecular epidemiological study on tetracycline resistance R plasmids in enterohaemorrhagic Escherichia coli O157[ratio ]H7

  • S. MAKINO (a1), H. ASAKURA (a1), T. OBAYASHI (a1), T. SHIRAHATA (a1), T. IKEDA (a2) and K. TAKESHI (a2)...

Abstract

Restriction patterns obtained with EcoRI and Southern hybridization were used for the differentiation of tetracycline-resistant (Tetr) R plasmids in enterobaemorrhagic Escherichia coli (EHEC) O157[ratio ]H7 isolates from a mass outbreak at a kindergarten in Obihiro-City, Hokkaido, Japan, 1996. Two kinds of Tetr R plasmids of 50 and 95 kb were detected. The 50-kb plasmids were identical to each other, while the 93-kb plasmids were of three types that were very similar to each other. The tet genes of both 50- and 95-kb R plasmids were 100% identical to the tet gene of pSC101 and all plasmids hybridized to a probe for tet. Because food-origin O157 strains were sensitive to tetracycline, we concluded that such Tetr R-plasmids might transfer to drug-sensitive O157 strains in the infected individuals.

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