Hostname: page-component-8448b6f56d-t5pn6 Total loading time: 0 Render date: 2024-04-25T06:14:48.364Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular epidemiology of human rotavirus

Published online by Cambridge University Press:  19 October 2009

P. Cash ,
E. Freebain ,
T. Brown  and
T. M. S. Reid
P. Cash
Affiliation:
From Department of Bacteriology, University of Aberdeen, Foresterhill, Aberdeen
E. Freebain
Affiliation:
From Department of Bacteriology, University of Aberdeen, Foresterhill, Aberdeen
T. Brown
Affiliation:
From Department of Bacteriology, University of Aberdeen, Foresterhill, Aberdeen
T. M. S. Reid
Affiliation:
Regional Laboratory, The City Hospital, Aberdeen
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The epidemiology of human rotavirus (HRV) in north-east Scotland was investigated between 1982 and 1984. During this period 708 HRV infections were recorded. The majority (83%) of the infections were in children less than 5 years of age although some were recorded in adults. The peak incidence occurred in the winter months although a high number of HRV infections was reported during the summer of 1983. A total of 840 faecal specimens containing rotavirus were screened for HRV genome RNA by polyacrylamide gel electrophoresis. Seven hundred (83%) specimens gave RNA profiles suitable for establishing the HRV electrophoretype. Twenty-five different electrophoretypes were identified, of which 21 had ‘long’ RNA profiles and four had ‘short’ RNA profiles. There was extensive co-circulation of distinct electrophoretypes although during any one epidemic period the majority of viruses belonged to a relatively small number of types. Comparison of viruses collected from hospitalized and non-hospitalized Patients showed no differences in electrophoretype distribution. HRV was identified in faecal specimens from a wide age group and no correlation was demonstrated between age of patient and electrophoretype of the infecting virus.

Type
Research Article
Information
Journal of Hygiene , Volume 96 , Issue 2 , April 1986 , pp. 265 - 275
Copyright
Copyright © Cambridge University Press 1986

References

REFERENCES

Beards, G. M. (1982). Polymorphism of genomic RNAs with rotavirus serotypes and subtypes. Archives of Virology 74, 65–70.CrossRefGoogle Scholar
Beards, G. M., Pilfold, J. N., Thouless, M. E. & Flewktt, T. H. (1980). Rotavirus serotypes by serum neutralisation. Journal of Medical Virology 5, 231–237.CrossRefGoogle ScholarPubMed
Bishop, R. F., Davidson, G. P., Holmes, I. H. & Ruck, B. J. (1973). Virus particles in the epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis. Lancet ii, 1281–1283.CrossRefGoogle Scholar
Brandt, C. D., Kim, H. W., Yolken, R. H., Kapikian, A. Z., Arrobio, J. O., Rodriguez, W. J., Wyatt, R. G., Chanock, R. M. & Parrott, R. H. (1979). Comparative epidemiology of two rotavirus serotypes and other viral agents associated with pediatric gastroenteritis. American Journal of Epidemiology 110, 243–254.CrossRefGoogle ScholarPubMed
Bryden, A. S., Davies, H. A., Hadley, R. E., Flewett, T. H., Morris, C. A. & Oliver, P. (1975). Rotavirus enteritis in the West Midlands during 1974. Lancet ii, 241–243.CrossRefGoogle Scholar
Cash, P. (1982). Human rotavirus RNA prepared from stool samples by a simple procedure suitable for the determination of electropherotypes. Journal of Virological Methods 4, 107–115.Google Scholar
Clarke, I. N. & McCrae, M. A. (1982). Structural analysis of electrophoretic variation in the genome profiles of rotavirus field isolates. Infection and Immunity 36, 492–497.CrossRefGoogle ScholarPubMed
Cubitt, W. D. & Holzel, H. (1980). An outbreak of rotavirus infection in a long-stay ward of a geriatric hospital. Journal of Clinical Pathology 33, 306–308.CrossRefGoogle Scholar
Estes, M. K., Graham, D. Y. & Dimitrov, D. H. (1984). The molecular epidemiology of rotavirus gastroenteritis. Progress in Medical Virology 29, 1–22.Google Scholar
Follet, E. A. C., Sanders, R. C., Beards, G. M., Hundley, F. & Desselberger, U. (1984). Molecular epidemiology of human rotaviruses. Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83. Journal of Hygiene 92, 209–222.Google Scholar
Garbarg-Chenon, A., Bricout, F. & Nicolas, J.-C. (1984). Study of genetic reassortment between two human rotaviruses. Virology 139, 358–365.CrossRefGoogle ScholarPubMed
Kalica, A. R., Greenberg, H. B., Espejo, R. T., Flores, J., Wyatt, R. G., Kapikian, A. Z. & Chanock, R. M. (1981 a). Distinctive ribonucleic acid patterns of human rotavirus subgroups 1 and 2. Infection and Immunity 33, 958–965.Google Scholar
Kalica, A. R., Greenberg, H. B., Wyatt, R. G., Flores, J., Sereno, M. M., Kapikian, A. Z. & Chanock, R. M. (1981 b). Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralisation and subgroup antigens. Virology 112, 385–390.Google Scholar
Killen, H. M. & Dimmock, N. J. (1982). Identification of a neutralisation-specific antigen of a calf rotavirus. Journal of Genereal Virology, 62, 297–311.CrossRefGoogle Scholar
Konno, T., Sato, T., Suzuki, H., Kitaoka, S., Katsushima, N., Sakamoto, M., Yazaki, N. & Ishida, N. (1984). Changing RNA patterns in rotaviruses of human origin: Demonstration of a single dominant pattern at the start of an epidemic and various patterns thereafter. Journal of Infectious Diseases 149, 683–687.CrossRefGoogle ScholarPubMed
Middleton, P. J., Szymanski, M. T. & Petric, M. (1977). Viruses associated with acute gastroenteritis in young children. American Journal of Diseases of Children 131, 733–737.Google ScholarPubMed
Rodger, S. M., Bishop, R. F., Birch, C., McLean, B. & Holmes, I. H. (1981). Molecular epidemiology of human rotaviruses in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid. Journal of Clinical Microbiology 13, 272–278.Google Scholar
Soenarto, Y., Sebono, T., Ridho, R., Alrasjid, H., Rhode, J. E., Bugg, H. C., Barnes, G. L. & Bishop, R. F. (1981). Acute diarrhea and rotavirus infection in newborn babies and children in Yogyakarta, Indonesia, from June 1978 to June 1979. Journal of Clinical Microbiology 14, 123–129.CrossRefGoogle ScholarPubMed
Spencer, E., Avendano, F. & Araya, M. (1983). Characteristics and analysis of electropherotypes of human rotavirus isolated in Chile. Journal of Infectious Diseases 148, 41–48.CrossRefGoogle ScholarPubMed
Wyatt, R. G., James, H. D., Pittmann, A. L., Hoshino, T., Greenberg, H. B., Kalica, A. R., Flores, J. & Kapikian, A. Z. (1983). Direct isolation in tissue culture of human rotaviruses and their characterisation into four serotypes. Journal of Clinical Microbiology 18, 310–317.CrossRefGoogle Scholar