Molecular typing of Mycoplasma pneumoniae strains by PCR-based methods and pulsed-field gel electrophoresis. Application to French and Danish isolates

A. COUSIN-ALLERY; A. CHARRON; B. DE BARBEYRAC; G. FREMY; J. SKOV JENSEN; H. RENAUDIN; C. BEBEAR

doi:10.1017/S0950268899003313

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Restriction fragment length polymorphism (RFLP) analysis of the amplified P1 gene was used to type 153 strains of Mycoplasma pneumoniae isolated in France between 1977 and 1994, and in Denmark between 1962 and 1994, and an additional group of 28 strains isolated from Belgium and Germany between 1990 and 1993. Random amplified polymorphic DNA (RAPD) analysis was tested on French, Belgian and German strains. Both methods separated the strains into two groups corresponding to the two reference strains M129 (group I) and FH (group II), and gave concordant results. When 75 selected strains of different geographical origin were analysed by pulsed-field gel electrophoresis (PFGE), strains of group II fell into two closely related subgroups, subgroup IIa corresponding to the reference strain FH, and subgroup IIb. Most of the strains isolated in Denmark in the period 1962–86 belonged to group I. Almost all strains isolated in France and Denmark between 1987 and 1988 were from group II, the two subgroups being present. In 1991–3, almost all strains from France as well as Denmark, Germany and Belgium belonged to group I.

Crossref Citations

This article has been cited by the following publications. This list is generated based on data provided by Crossref.

O'Brien, A. D. Dorigo-Zetsma, J. Wendelien Wilbrink, Berry Dankert, Jacob and Zaat, Sebastian A. J. 2001. Mycoplasma pneumoniae P1 Type 1- and Type 2-Specific Sequences within the P1 Cytadhesin Gene of Individual Strains . Infection and Immunity, Vol. 69, Issue. 9, p. 5612.

Marois, Corinne Dufour-Gesbert, Fabienne and Kempf, Isabelle 2001. Comparison of pulsed-field gel electrophoresis with random amplified polymorphic DNA for typing of Mycoplasma synoviae. Veterinary Microbiology, Vol. 79, Issue. 1, p. 1.

Jacobs, Enno 2002. Molecular Biology and Pathogenicity of Mycoplasmas. p. 519.

Razin, Shmuel 2002. Molecular Biology and Pathogenicity of Mycoplasmas. p. 531.

Waites, Ken B. and Talkington, Deborah F. 2004. Mycoplasma pneumoniaeand Its Role as a Human Pathogen. Clinical Microbiology Reviews, Vol. 17, Issue. 4, p. 697.

Dumke, Roger Catrein, Ina Herrmann, Richard and Jacobs, Enno 2004. Preference, adaptation and survival of Mycoplasma pneumoniae subtypes in an animal model. International Journal of Medical Microbiology, Vol. 294, Issue. 2-3, p. 149.

Daxboeck, Florian 2004. Encyclopedia of Medical Genomics and Proteomics. p. 876.

Pereyre, S. Guyot, C. Renaudin, H. Charron, A. Bébéar, C. and Bébéar, C. M. 2004. In Vitro Selection and Characterization of Resistance to Macrolides and Related Antibiotics in Mycoplasma pneumoniae . Antimicrobial Agents and Chemotherapy, Vol. 48, Issue. 2, p. 460.

Melin, Anne-Marie Allery, Annie Perromat, Annie Bébéar, Christiane Déléris, Gérard and de Barbeyrac, Bertille 2004. Fourier transform infrared spectroscopy as a new tool for characterization of mollicutes. Journal of Microbiological Methods, Vol. 56, Issue. 1, p. 73.

Morozumi, Miyuki Hasegawa, Keiko Kobayashi, Reiko Inoue, Nagako Iwata, Satoshi Kuroki, Haruo Kawamura, Naohisa Nakayama, Eiichi Tajima, Takeshi Shimizu, Kouichi and Ubukata, Kimiko 2005. Emergence of Macrolide-Resistant Mycoplasma pneumoniae with a 23S rRNA Gene Mutation . Antimicrobial Agents and Chemotherapy, Vol. 49, Issue. 6, p. 2302.

Bébéar, Cécile M. and Bébéar, Christiane 2006. Mycoplasmes. EMC - Biologie Médicale, Vol. 1, Issue. 1, p. 1.

Broaders, Samantha A. Hooper, W. Craig Phillips, Donald J. and Talkington, Deborah F. 2006. Mycoplasma pneumoniae subtype-independent induction of proinflammatory cytokines in THP-1 cells. Microbial Pathogenesis, Vol. 40, Issue. 6, p. 286.

Dumke, Roger Lück, Paul Christian Noppen, Christoph Schaefer, Christoph von Baum, Heike Marre, Reinhard and Jacobs, Enno 2006. Culture-Independent Molecular Subtyping of Mycoplasma pneumoniae in Clinical Samples . Journal of Clinical Microbiology, Vol. 44, Issue. 7, p. 2567.

Musatovova, Oxana Herrera, Caleb and Baseman, Joel B. 2006. Proximal Region of the Gene Encoding Cytadherence-Related Protein Permits Molecular Typing of Mycoplasma genitalium Clinical Strains by PCR-Restriction Fragment Length Polymorphism . Journal of Clinical Microbiology, Vol. 44, Issue. 2, p. 598.

Bébéar, Christiane and Bébéar, Cécile M. 2007. Infections humaines a mycoplasmes. Revue Francophone des Laboratoires, Vol. 2007, Issue. 391, p. 63.

Pereyre, S. Charron, A. Renaudin, H. Bébéar, C. and Bébéar, C. M. 2007. First Report of Macrolide-Resistant Strains and Description of a Novel Nucleotide Sequence Variation in the P1 Adhesin Gene in Mycoplasma pneumoniae Clinical Strains Isolated in France over 12 Years . Journal of Clinical Microbiology, Vol. 45, Issue. 11, p. 3534.

Bébéar, C.-M. 2007. Physiopathologie et diagnostic des infections à Mycoplasma pneumoniae. Revue Française d'Allergologie et d'Immunologie Clinique, Vol. 47, Issue. 7, p. 438.

Waites, Ken B. Simecka, Jerry W. Talkington, Deborah F. and Atkinson, T. Prescott 2007. Community-Acquired Pneumonia. p. 183.

Musatovova, Oxana Kannan, T. R. and Baseman, Joel B. 2008. Genomic Analysis Reveals Mycoplasma pneumoniae Repetitive Element 1-Mediated Recombination in a Clinical Isolate . Infection and Immunity, Vol. 76, Issue. 4, p. 1639.

Atkinson, Thomas Prescott Balish, Mitchell F. and Waites, Ken B. 2008. Epidemiology, clinical manifestations, pathogenesis and laboratory detection ofMycoplasma pneumoniaeinfections: Figure 1. FEMS Microbiology Reviews, Vol. 32, Issue. 6, p. 956.

Download full list

Article contents

Molecular typing of Mycoplasma pneumoniae strains by PCR-based methods and pulsed-field gel electrophoresis. Application to French and Danish isolates

Abstract

Save article to Kindle

Save article to Dropbox

Save article to Google Drive

Reply to: Submit a response

Your details

You have entered the maximum number of contributors

Conflicting interests