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A novel multiplex-PCR for the rapid identification of Mycobacterium bovis in clinical isolates of both veterinary and human origin

Published online by Cambridge University Press:  25 June 2003

L. COBOS-MARÍN ,
J. MONTES-VARGAS ,
S. RIVERA-GUTIERREZ ,
A. LICEA-NAVARRO ,
J. A. GONZÁLEZ-Y-MERCHAND  and
I. ESTRADA-GARCÍA
L. COBOS-MARÍN
Affiliation:
Departamento de Inmunologia, Escuela Nacional de Ciencias Biologicas, IPN; Prolongacion Carpio y Plan de Ayala s/n, Col. Sto. Tomas, Mexico D.F., 11340, Mexico
J. MONTES-VARGAS
Affiliation:
CANETB, SAGARPA, Av. Del charro 770-1sur Cd Juarez, Chih. 32320, México
S. RIVERA-GUTIERREZ
Affiliation:
Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, IPN; Prolongacion Carpio y Plan de Ayala s/n, Col. Sto. Tomas, Mexico D.F., 11340, Mexico
A. LICEA-NAVARRO
Affiliation:
Departamento de Acuicultura y Biotecnología Marina. CICESE, Km 107 Carretera Tijuana-Ensenada, Ensenada, B.C., Mexico
J. A. GONZÁLEZ-Y-MERCHAND
Affiliation:
Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, IPN; Prolongacion Carpio y Plan de Ayala s/n, Col. Sto. Tomas, Mexico D.F., 11340, Mexico
I. ESTRADA-GARCÍA
Affiliation:
Departamento de Inmunologia, Escuela Nacional de Ciencias Biologicas, IPN; Prolongacion Carpio y Plan de Ayala s/n, Col. Sto. Tomas, Mexico D.F., 11340, Mexico
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Abstract

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Bovine tuberculosis is a zoonotic disease that not only causes huge economic losses but also poses an important risk for human infection. The definitive identification of a clinical isolate relies on time-consuming, highly specialized and laborious biochemical tests. We have developed a method for the rapid and reliable identification of Mycobacterium bovis and for its simultaneous differentiation from other members of the M. tuberculosis complex. Furthermore, the technique also allowed us to distinguish M. tuberculosis complex members from other Mycobacterial species. The method comprises both a single PCR and a multiplex-PCR and can be confidently applied to samples of both veterinary and human origin.

Type
Research Article
Information
Epidemiology & Infection , Volume 130 , Issue 3 , June 2003 , pp. 485 - 490
Copyright
© 2003 Cambridge University Press