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Phylogenetic and epidemiological analysis of Neisseria meningitidis using DNA probes

Published online by Cambridge University Press:  15 May 2009

H. Ni ,
A. I. Knight ,
K. A. V. Cartwright  and
J. J. McFadden
H. Ni
Affiliation:
Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, Surrey, GU2 5XH, UK
A. I. Knight
Affiliation:
Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, Surrey, GU2 5XH, UK
K. A. V. Cartwright
Affiliation:
Public Health Laboratory, Gloucester Royal Hospital, Great Western Rd, Gloucester, GL1 3NN
J. J. McFadden*
Affiliation:
Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, Surrey, GU2 5XH, UK
*
*Author for correspondence.
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Summary

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The genetic relationships between various serotypes and serogroups of meningococcal strains were investigated by restriction fragment-length polymorphism (RFLP) analysis using a number of random DNA probes and a probe containing a truncated copy of the meningococcal insertion sequence IS1106. The data were used to estimate genetic distance between all pairs of strains and to construct phylogenetic trees for meningococcal strains. B15: P1.16R strains isolated from cases of systemic meningococcal disease in two health districts with a high incidence of disease were clonal in contrast to similar strains from cases occurring in other parts of the UK. Strains from these areas, which contain a similar genomic deletion, were found to be derived from two distinct lineages within the B15: P1.16R phylogenetic group. RFLP data demonstrated that present serological typing systems for the meningoccus do not necessarily reflect true genetic relationships.

Type
Research Article
Information
Epidemiology & Infection , Volume 109 , Issue 2 , October 1992 , pp. 227 - 239
Copyright
Copyright © Cambridge University Press 1992

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