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The preparation of simulated water samples for the purpose of bacteriological quality control

Published online by Cambridge University Press:  15 May 2009

R. D. Gray  and
G. H. Lowe
R. D. Gray
Affiliation:
Public Health Laboratory, Newport, Gwent
G. H. Lowe
Affiliation:
Public Health Laboratory, Newport, Gwent
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Summary

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As part of the bacteriological quality control programme of the Public Health Laboratory Service, we were asked to investigate the possibility of providing simulated water samples for distribution to the laboratories. For this purpose it was necessary to find some means whereby suspensions of coliform organisms and Escherichia coli could be kept relatively stable in number at room temperature for a period of 7–10 days. This, it was finally found, was best achieved by adding selected strains of the organisms to improved formate lactose glutamate medium (Gray, 1964) without the lactose but with added boric acid to a final concentration of 1·8%.

The procedures adopted in the successful quality control programme are described.

Information

Type
Research Article
Information
Journal of Hygiene , Volume 76 , Issue 1 , February 1976 , pp. 49 - 56
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

American Public Health Association (1955). Standard Methods for the Examination of Water, Sewage and Industrial Wastes 10th ed. New York: American Public Health Association.Google Scholar
Gray, R. D. (1964). An improved formate lactose glutamate medium for the detection of Escherichia coli and other coliform organisms in water. Journal of Hygiene 62, 495.Google ScholarPubMed
Hoather, R. C. (1952). The bacteriological examination of water with particular reference to the stability of the numbers of coli-aerogenes, including Bact. coli, during storage of samples overnight. Journal of the Institution of Water Engineers 6, 426.Google Scholar
Hoather, R. C. (1957). The effect of thiosulphate and of phosphate on the bactericidal action of copper and zinc in samples of water. Journal of Applied Bacteriology 20, 180.CrossRefGoogle Scholar
Miles, A. A. & Misra, S. S. (1938). The estimation of the bactericidal power of the blood. Journal of Hygiene 38, 732.Google ScholarPubMed
Porter, I. A. & Brodie, J. (1969). Boric acid preservation of urine samples. British Medical Journal ii, 353.CrossRefGoogle Scholar
Report (1952). Public Health Laboratory Service Water Sub-committee. The effect of storage on the coliform and Bacterium coli counts of water samples. Overnight storage at room and refrigerator temperature. Journal of Hygiene 50, 107.CrossRefGoogle Scholar
Report (1953). Public Health Laboratory Service Water Sub-committee. The effect of storage on the coliform and Bacterium coli counts of water samples. Storage for six hours at room and refrigerator temperature. Journal of Hygiene 51, 559.Google Scholar
Report (1969). The Bacteriological Examination of Water Supplies. Reports on Public Health and Medical Subjects no. 71. London: H.M.S.O.Google Scholar