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Pseudomonas pseudomallei isolates collected over 25 years from a non-tropical endemic focus show clonality on the basis of ribotyping

Published online by Cambridge University Press:  15 May 2009

B. Currie ,
H. Smith-Vaughan ,
C. Golledge ,
N. Buller ,
K. S. Sriprakash  and
D. J. Kemp
B. Currie*
Affiliation:
Menzies School of Health Research and Royal Darwin Hospital, Darwin, Australia
H. Smith-Vaughan
Affiliation:
Menzies School of Health Research and Royal Darwin Hospital, Darwin, Australia
C. Golledge
Affiliation:
Sir Charles Gairdner Hospital, Perth, Australia
N. Buller
Affiliation:
Western Australian Department of Agriculture, Perth, Australia
K. S. Sriprakash
Affiliation:
Menzies School of Health Research and Royal Darwin Hospital, Darwin, Australia
D. J. Kemp
Affiliation:
Menzies School of Health Research and Royal Darwin Hospital, Darwin, Australia
*
* Correspondence and reprints: Dr Bart Currie, Menzies School of Health Research, P.O. Box 41096. Casuarina NT 0811, Australia.
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Summary

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Between 1966 and 1991, melioidosis, a disease caused by Pseudomonas pseudomallei that is mostly confined to tropical regions, occurred in farm animals and a farmer in temperate south-west Western Australia. Using an Escherichia coli probe containing a ribosomal RNA operon, P. pseudomallei DNA from isolates from 8 animals, a soil sample and the human case showed an identical ribotype on Southern blotting. The ribotype was different from the 3 commonest ribotypes seen in tropical Australia. This molecular typing supports the theory of clonal introduction of P. pseudomallei into a non-endemic region, with environmental contamination, local dissemination and persistence over 25 years. As melioidosis is often fatal in humans, such persistence in a temperate region is cause for concern.

Type
Research Article
Information
Epidemiology & Infection , Volume 113 , Issue 2 , October 1994 , pp. 307 - 312
Copyright
Copyright © Cambridge University Press 1994

References

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