Skip to main content Accessibility help
×
×
Home

Pseudomonas pseudomallei isolates collected over 25 years from a non-tropical endemic focus show clonality on the basis of ribotyping

  • B. Currie (a1), H. Smith-Vaughan (a1), C. Golledge (a2), N. Buller (a3), K. S. Sriprakash (a1) and D. J. Kemp (a1)...

Summary

Between 1966 and 1991, melioidosis, a disease caused by Pseudomonas pseudomallei that is mostly confined to tropical regions, occurred in farm animals and a farmer in temperate south-west Western Australia. Using an Escherichia coli probe containing a ribosomal RNA operon, P. pseudomallei DNA from isolates from 8 animals, a soil sample and the human case showed an identical ribotype on Southern blotting. The ribotype was different from the 3 commonest ribotypes seen in tropical Australia. This molecular typing supports the theory of clonal introduction of P. pseudomallei into a non-endemic region, with environmental contamination, local dissemination and persistence over 25 years. As melioidosis is often fatal in humans, such persistence in a temperate region is cause for concern.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Pseudomonas pseudomallei isolates collected over 25 years from a non-tropical endemic focus show clonality on the basis of ribotyping
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Pseudomonas pseudomallei isolates collected over 25 years from a non-tropical endemic focus show clonality on the basis of ribotyping
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Pseudomonas pseudomallei isolates collected over 25 years from a non-tropical endemic focus show clonality on the basis of ribotyping
      Available formats
      ×

Copyright

Corresponding author

* Correspondence and reprints: Dr Bart Currie, Menzies School of Health Research, P.O. Box 41096. Casuarina NT 0811, Australia.

References

Hide All
1.Leelarasamee, A, Bovornkitt, SMelioidosis: review and update. Rev Infect Dis 1989; 11: 413–25.
2.Dance, DABMelioidosis: the tip of the iceberg? Clin Microbiol Rev 1991; 4: 5260.
3.Cottew, GSMelioidosis in sheep in Queensland. A description of the casual organism. Aust J Exp Biol Med Sci 1950; 28: 677–83.
4.Rimington, RAMelioidosis in north Queensland. Med J Aust 1962; 1: 50–3.
5.Currie, BJMedicine in tropical Australia. Med J Aust 1993; 158: 609–15.
6.Ketterer, PJ, Bamford, VWA case of melioidosis in lambs in south Western Australia. Aust Vet J 1967; 43: 7980.
7.Golledge, CL, Chin, WS, Tribe, AE, Condon, RJ, Ashdown, LRA case of human melioidosis originating in south-west Western Australia. Med J Aust 1992; 157: 332–4.
8.Lew, AE, Desmarchelier, PMMolecular typing of Pseudomonas pseudomallei: restriction fragment length polymorphisms of rRNA genes. J Clin Microbiol 1993; 31: 533–9.
9.Pitcher, DG, Saunders, NA, Owen, RJRapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 1989; 8: 151–6.
10.Feinberg, AP, Vogelstein, BA technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 1983; 132: 613.
11.Altwegg, M, Hickman-Brenner, FW, Farmer, JJRibosomal RNA gene restriction patterns provide increased sensitivity for typing Salmonella typhi strains. J Infect Dis 1989; 160: 145–9.
12.Sutmoller, P, Kraneveld, FC, van der Schaaf, AMelioidosis (Pseudomalleus) in sheep, goats and pigs on Aruba Netherlands Antilles. J Am Vet Med Assoc 1957; 130: 415–7.
13.Mollaret, HH‘L'affaire du Jardin des plantes’ ou comment la melioidose fit son apparition en France. Med Mai Infect 1988; 18: 643–54.
14.Ketterer, PJ, Donald, B, Rogers, RJBovine melioidosis in south-eastern Queensland. Aust Vet J 1975; 51: 395–8.
15.Dance, DAB, King, C, Aucken, H, Knott, CD, West, PG, Pitt, TLAn outbreak of melioidosis in imported primates in Britain. Vet Rec 1992; 130: 525–9.
16.Desmarchelier, PM, Dance, DAB, Chaowagul, W, Suputtamongkol, Y, White, NJ, Pitt, TLRelationships among Pseudomonas pseudomallei isolates from patients with recurrent melioidosis. J Clin Microbiol 1993; 31: 1592–6.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed