Skip to main content Accessibility help
×
Home

RAPD analysis of isolates of Burkholderia pseudomallei from patients with recurrent melioidosis

  • A. Haase (a1), A. Melder (a1), H. Smith-Vaughan (a1), D. Kemp (a1) and B. Currie (a1)...

Summary

Twenty-seven isolates of Burholderia pseudomallei (formerly Pseudomonas pseudomallei) from ten patients with recurrent melioidosis were analysed by RAPD. In two cases RAPD patterns in recurrent isolates differed from the original isolates; one was considered a likely reinfection while the other may represent relapse from one of two strains initially infecting the patient. In two cases where a change in antibiotic resistance had occurred between original and relapse isolates, slight changes in RAPD patterns were found with one of the four primers used. In the other six cases the relapse was clearly due to the original strain re-emerging unchanged, with identical RAPD patterns with all four primers.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      RAPD analysis of isolates of Burkholderia pseudomallei from patients with recurrent melioidosis
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      RAPD analysis of isolates of Burkholderia pseudomallei from patients with recurrent melioidosis
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      RAPD analysis of isolates of Burkholderia pseudomallei from patients with recurrent melioidosis
      Available formats
      ×

Copyright

References

Hide All
1.Rode, JW, Webling, DD. Melioidosis in the Northern Territory of Australia. Med J Aust 1981; 1: 181–4.
2.Chaowagul, W, White, NJ, Dance, DAB et al. , Melioidosis: a major cause of community-acquired septicemia in northern Thailand. J Infect Dis 1989; 159: 890–9.
3.Currie, B. Medicine in Tropical Australia. Med J Aust 1993; 158: 609–15.
4.Desmarchelier, PM, Dance, DAB, Chaowagul, W, Supputamongkol, Y, White, NJ. Pitt, TL. Relationships among Pseudomonas pseudomallei isolates from patients with recurrent melioidosis. J Clin Microbiol 1993; 31: 1592–6.
5.Morrison, RE, Lamb, AS, Craig, DB. Melioidosis: a reminder. Am J Med 1988; 84: 965–7.
6.Bouvy, JJ, Degener, JE, Stijnen, C, Gallee, MP. Septic melioidosis after a visit to Southeast Asia. Eur J Clin Microbiol 1986; 5: 655–6.
7.Leelarasamee, A, Bovornkitti, S. Melioidosis: review and update. Rev Infect Dis 1989; 11: 413–25.
8.Weber, DR, Douglass, LE, Brundage, WG, Stallkamp, TC. Acute varieties of melioidosis occurring in US soldiers in Vietnam. Am J Med 1969; 46: 234.
9.Wilks, D, Kim Jacobson, S, Lever, AM, Farrington, M. Fatal melioidosis in a tourist returning from Thailand. J Infect 1994; 29: 8790.
10.Mays, EE, Ricketts, EA. Melioidosis: recrudescence associated with bronchogenic carcinoma twenty-six years following initial geographic exposure. Chest 1976; 68: 261–3.
11.Chaowagul, W, Supputtamongkol, Y, Dance, DAB, Rajchanuvong, A. Relapse in melioidosis: incidence and risk factors. J Infect Dis 1993; 168, 1181–5.
12.Currie, B, Howard, D, The, VN, Withnall, K, Merianos, A. The 1990–1991 outbreak of melioidosis in the Northern Territory of Australia: clinical aspects. S A J Trop Med Publ Health 1993; 24: 436–43.
13.Currie, B, Smith-Vaughan, H, Golledge, C, Buller, N, Sriprakash, KS, Kemp, DJ. Pseudomonas pseudomallei isolates collected over 25 years from a non-tropical endemic focus show clonality on the basis of ribotyping. Epidemiol Infect 1994; 113: 307–12.
14.Williams, JGK, Kubelik, AR, Livak, KJ, Rafalski, JA, Tingley, SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 1990; 18: 6531–5.
15.Welsh, J, McClelland, M. Fingerprinting genomes using PCR with arbitrary primers. Nucl Acids Res 1990; 18: 7213–8.
16.Akopyanz, N, Bukanov, BO, Westblom, TU. Kresovich, S. Berg, DE. DNA diversity among clinical isolates of Helicobacter pylori detected bv PCR-based RAPD fingerprinting. Nucl Acids Res 1992; 20: 5137–42.
17.Kersulyte, D, Woods, JP, Keath, EJ, Goldman, WE, Berg, DE. Diversity among clinical isolates of Histoplasma capsulatum detected by polymerase chain reaction with arbitrary primers. J Bact 1992; 174: 7075–9.
18.Haase, A, Smith-Vaughan, H, Melder, A et al. , Subdivision of Burkholderia pseudomallei ribotypes into multiple types by RAPD analysis provides new insights into its epidemiology. Submitted for publication.
19.Pitcher, DG, Saunders, NA, Owen, RJ. Rapid extraction of bacterial genomic DNA with guanidinium thiocyanate. Lett Appl Microbiol 1989; 8: 151–6.
20.Lew, AE, Desmarchelier, PM. Molecular typing of Pseudomonas pseudomallei: restriction fragment length polymorphism of rRNA genes. J Clin Microbiol 1993; 31: 533–9.
21.Weis, SE, Slocum, PC, Blais, FX et al. , The effect of directly observed therapy on the rates of drug resistance and relapse in tuberculosis. N Engl J Med 1994; 330: 1179–84.

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed