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Real-time investigation of a Legionella pneumophila outbreak using whole genome sequencing

  • R. M. A. GRAHAM (a1), C. J. DOYLE (a1) and A. V. JENNISON (a1)
Summary

Legionella pneumophila is the main pathogen responsible for outbreaks of Legionnaires' disease, which can be related to contaminated water supplies such as cooling towers or water pipes. We combined conventional molecular methods and whole genome sequence (WGS) analysis to investigate an outbreak of L. pneumophila in a large Australian hospital. Typing of these isolates using sequence-based typing and virulence gene profiling, was unable to discriminate between outbreak and non-outbreak isolates. WGS analysis was performed on isolates during the outbreak, as well as on unlinked isolates from the Public Health Microbiology reference collection. The more powerful resolution provided by analysis of whole genome sequences allowed outbreak isolates to be distinguished from isolates that were temporally and spatially unassociated with the outbreak, demonstrating that this technology can be used in real-time to investigate L. pneumophila outbreaks.

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Copyright
Corresponding author
* Author for correspondence: Dr R. M. A. Graham, Public Health Microbiology, Communicable Disease, PO Box 594, Department of Health, Forensic and Scientific Services, Brisbane, QLD 4108, Australia. (Email: rikki_graham@health.qld.gov.au)
References
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1. Fields, BS, Benson, RF, Besser, RE. Legionella and Legionnaires' disease: 25 years of investigation. Clinical Microbiology Reviews 2002; 15: 506526.
2. Gomez-Valero, L, et al. Extensive recombination events and horizontal gene transfer shaped the Legionella pneumophila genomes. BMC Genomics 2011; 12: 536.
3. Yu, VL, et al. Distribution of Legionella species and serogroups isolated by culture in patients with sporadic community-acquired legionellosis: an international collaborative survey. Journal of Infectious Diseases 2002; 186: 127128.
4. Huang, B, et al. A predominant and virulent Legionella pneumophila serogroup 1 strain detected in isolates from patients and water in Queensland, Australia, by an amplified fragment length polymorphism protocol and virulence gene-based PCR assay. Journal of Clinical Microbiology 2004; 42: 41644168.
5. Robinson, E, Walker, T, Pallen, M. Genomics and outbreak investigation: from sequence to consequence. Genome Medicine 2013; 5: 36.
6. Reuter, S, et al. A pilot study of rapid whole-genome sequencing for the investigation of a Legionella outbreak. BMJ Open 2013; 3(1).
7. Ginevra, C, et al. Legionella pneumophila sequence type 1/Paris pulsotype subtyping by spoligotyping. Journal of Clinical Microbiology 2012; 50: 696701.
8. Pannier, K, Heuner, K, Lück, C. Variable genetic element typing: a quick method for epidemiological subtyping of Legionella pneumophila. European Journal of Clinical Microbiology and Infectious Diseases 2010; 29: 481487.
9. Winn, WCJ (ed.). Legionella, 7th edn. Washington. DC: ASM Press, 1999, pp. 572585.
10. Edwards, MT, Fry, NK, Harrison, TG. Clonal population structure of Legionella pneumophila inferred from allelic profiling. Microbiology 2008; 154: 852864.
11. Huang, B, et al. Distribution of 19 major virulence genes in Legionella pneumophila serogroup 1 isolates from patients and water in Queensland, Australia. Journal of Medical Microbiology 2006; 55: 993997.
12. Cazalet, C, et al. Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity. Nature Genetics 2004; 36: 11651173.
13. D'Auria, G, et al. Legionella pneumophila pangenome reveals strain-specific virulence factors. BMC Genomics 2010; 11: 181.
14. Queensland Department of Health. Review of the prevention and control of Legionella pneumophila infection in Queensland (http://www.health.qld.gov.au/legionnaires/docs/cho-legionella-report.pdf). Queensland Government, 2013.
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Epidemiology & Infection
  • ISSN: -
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
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