Skip to main content
×
Home

The role of serum haemagglutination-inhibiting antibody in protection against challenge infection with influenza A2 and B viruses

  • D. Hobson (a1), R. L. Curry (a1), A. S. Beare (a2) and A. Ward-Gardner (a3)
Summary
SUMMARY

The intranasal inoculation of volunteers with living partially attenuated strains of influenza A and B viruses offers a new opportunity to determine the protective effect of serum haemagglutin-inhibiting antibody against a strictly homologous virus, under conditions where the time and dosage of the infective challenge can be controlled, the scoring of proven infections can be more precise and higher rates of infection can be achieved than in most natural epidemics.

In 1032 adult volunteers, whose serum HI antibody titre was determined immediately before virus challenge, there was a consistent inverse quantitative relationship between the HI titre and the likelihood of infection. The PD50 (50 % protective dose) of HI antibody was 1/18–1/36, but an unusual finding was that volunteers with no detectable pre-challenge antibody often seem to be less susceptible to infection than those with pre-challenge antibody in low titre.

In one group of volunteers challenged with an influenza B strain there was no evidence that pre-challenge antibody titres against viral neuraminidase had any significant protective effect against challenge infection.

Copyright
References
Hide All
Allison A. C. (1965). Genetic factors in resistance against virus infections. Archiv für die gesamte Virusforschung 17, 280.
Bashe W. J., Stegmuller H., Leonida D. & Greenwald P. (1964). Failure of polyvalent vaccine to provide clinical protection against Asian influenza. New England Journal of Medicine 270, 870.
Beare A. S., Hobson D., Reed S. E. & Tyrrell D. A. J. (1968). A comparison of live and killed influenza virus vaccines. Lancet ii, 418.
Beare A. S., Maassab H. F., Tyrrell D. A. J., Slepushkin A. N. & Hall T. S. (1971). A comparative study of attenuated influenza viruses. Bulletin of the World Health Organisation 44, 593.
Beare A. S., Tyrrell D. A. J., Hobson D., Howells C. H. L., Pereira M. S., Pollock T. M. & Tyler L. E. (1969). Live influenza B vaccine in volunteers. Journal of Hygiene 67, 1.
Downie J. C. (1970). Neuraminidase-inhibiting and haemagglutination-inhibiting antibodies in serum and nasal secretion of volunteers immunized with attenuated and inactivated influenza B/Eng/13/65 virus vaccines. Journal of Immunology 105, 620.
Downie J. C. & Stuart-Harris C. H. (1970). The production of neutralizing activity in serum and nasal secretion following immunization with influenza B virus. Journal of Hygiene 68, 233.
Easterday B., Laver W. G., Pereira H. G. & Schild G. C. (1969). Antigenic composition of recombinant virus strains produced from human and avian influenza viruses. Journal of General Virology 5, 83.
Hobson D., Lane C. A., Beare A. S. & Chivers C. P. (1964). Serological studies on adult volunteers inoculated with oil-adjuvant Asian influenza vaccine. British Medical Journal ii, 271.
Hoyle L. (1968). The Influenza Viruses, chap. 27. Vienna: Springer-Verlag.
Laver W. G. (1963). The structure of influenza viruses. 3. Disruption of the virus particle and separation of neuraminidase activity. Virology 20, 251.
Lindenmann J., Lane C. A. & Hobson D. (1963). The resistance of A2G mice to myxoviruses. Journal of Immunology 90, 942.
McDonald J. C., Zuckermann A. J., Beare A. S. & Tyrrell D. A. J. (1962). Trials of live influenza vaccine in the Royal Air Force. British Medical Journal i, 1036.
McDonald J. C. & Zuckermann A. J. (1962). A B O blood groups and acute respiratory virus disease. British Medical Journal ii, 89.
Maynard J. E., Dull H. B., Hanson M. L., Feltz E. T., Berger B. & Hammes L. (1968). Evaluation of monovalent and polyvalent influenza vaccines during an epidemic of Type A2 and B influenza. American Journal of Epidemiology 87, 148.
Meiklejohn G., Kempe C. H., Thalman W. G. & Lennette E. H. (1952). Evaluation of monovalent influenza vaccines. II. Observations during an influenza A-prime epidemic. American Journal of Hygiene 55, 12.
Potter C. W. (1969). HI antibody to various influenza viruses and adenoviruses in individuals of blood groups A and O. Journal of Hygiene 67, 67.
Schild G. C. & Newman R. W. (1969). Antibody against influenza A2 virus neuraminidase in human sera. Journal of Hygiene 67, 353.
Slepushkin A. N., Schild G. C., Beare A. S., Chinn S. & Tyrrell D. A. J. (1971). Anti-neuraminidase antibody and resistance to vaccination with live A2/Hong Kong influenza virus vaccines. Journal of Hygiene 69, 571.
Tyrrell D. A. J. (1963). The use of volunteers. American Review of Respiratory Diseases 88, Supplement 128.
Webster R. G. & Laver W. G. (1967). Preparation and properties of antibody directed specifically against the neuraminidase of influenza virus. Journal of Immunology 99, 49.
World Health Organization (1953). Expert Committee on Influenza. Technical Report Series of the World Health Organization, no. 64.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 38 *
Loading metrics...

Abstract views

Total abstract views: 389 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 23rd November 2017. This data will be updated every 24 hours.