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Staphylococcal cassette chromosome mec (SCCmec) in methicillin-resistant coagulase-negative staphylococci. A review and the experience in a tertiary-care setting

  • E. GARZA-GONZÁLEZ (a1), R. MORFÍN-OTERO (a2), J. M. LLACA-DÍAZ (a3) and E. RODRIGUEZ-NORIEGA (a2)

Summary

Coagulase-negative staphylococci (CNS) are increasingly recognized to cause clinically significant infections, with S. epidermidis often cited as the third most common cause of nosocomial sepsis. Among CNS, there is a high prevalence of methicillin resistance associated with staphylococcal cassette chromosome (SCCmec) elements. Although identical SCCmec types can exist in S. aureus and CNS, some novel classes of SCCmec may be unique to CNS. Differences in the accuracy of identification of CNS species and use of non-standardized methods for the detection of methicillin resistance have led to confusing data in the literature. In addition to the review of SCCmec in CNS, in this paper we report a 2-year surveillance of methicillin-resistant CNS in a tertiary-care hospital in Guadalajara, Mexico.

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Corresponding author

*Author for correspondence: Dra. E. Garza-González, Avenida Francisco I. Madero s/n. Colonia Mitras Centro, Monterrey, Nuevo Leon, 64460, Mexico. (Email: elvira_garza_gzz@yahoo.com)

References

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1.Bannerman, TL, Peacock, SJ. Staphylococcus, Micrococcus and other catalase positive cocci. In: Murray, PR, Baron, EJ, Jorgensen, JH, Pfaller, MA, Landry, ML, eds. Manual of Clinical Microbiology, 9th edn. Washington, DC: ASM Press, 2007, pp. 390404.
2.Euzeby, JP. List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. International Journal of Systematic Bacteriology 1997; 47: 590592.
3.Rogers, KL, Fey, PD, Rupp, ME. Coagulase-negative staphylococcal infections. Infection Disease Clinics of North America 2009; 23: 7398.
4.Huebner, J, Goldmann, DA. Coagulase-negative staphylococci: role as pathogens. Annual Review of Medicine 1999; 50: 223236.
5.McCann, MT, Gilmore, BF, Gorman, SP. Staphylococcus epidermidis device-related infections: pathogenesis and clinical management. Journal of Pharmacy and Pharmacology 2008; 60: 15511571.
6.Davey, ME, O'Toole, GA. Microbial biofilms: from ecology to molecular genetics. Microbiology and Molecular Biology Reviews 2000; 64: 847867.
7.Stewart, P, Costerton, J. Antibiotic resistance of bacteria in biofilms. Lancet 2001; 58: 135138.
8.Gill, SR, et al. Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain. Journal of Bacteriology 2005; 187: 24262438.
9.von Eiff, C, Peters, G, Heilmann, C. Pathogenesis of infections due to coagulase-negative staphylococci. Lancet Infectious Diseases 2002; 2: 677685.
10.Pfaller, MA, et al. Survey of blood stream infections attributable to gram-positive cocci: frequency of occurrence and antimicrobial susceptibility of isolates collected in 1997 in the United States, Canada, and Latin America from the SENTRY Antimicrobial Surveillance Program. SENTRY Participants Group. Diagnostic Microbiology and Infectious Disease 1999; 33: 283297.
11.Emory, TG, Gaynes, RP. An overview of nosocomial infections, including the role of the microbiology laboratory. Clinical Microbiology Reviews 1993; 6: 428442.
12.Diekema, DJ, et al. Survey of infections due to Staphylococcus species: frequency of occurrence and antimicrobial susceptibility of isolates collected in the United States, Canada, Latin America, Europe, and the Western Pacific region for the SENTRY Antimicrobial Surveillance Program, 1997–1999. Clinical Infectious Diseases 2001; 32 (Suppl. 2): S11432.
13.Barber, M. Methicillin-resistant staphylococci. Journal of Clinical Pathology 1961; 14: 385393.
14.Archer, GL, Mayhall, CG. Comparison of epidemiological markers used in the investigation of an outbreak of methicillin-resistant Staphylococcus aureus infections. Journal of Clinical Microbiology 1983; 18: 395399.
15.Rhinehart, E, et al. G. Nosocomial clonal dissemination of methicillin-resistant Staphylococcus aureus. Elucidation by plasmid analysis. Archives of Internal Medicine 1987; 147: 521524.
16.Katayama, Y, Ito, T, Hiramatsu, K. A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 2000; 44: 15491555.
17.Ito, T, Katayama, Y, Hiramatsu, K. Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315. Antimicrobial Agents and Chemotherapy 1999; 43: 14491458.
18.Katayama, Y, Ito, T, Hiramatsu, K. Genetic organization of the chromosome region surrounding mecA in clinical staphylococcal strains: role of IS431-mediated mecI deletion in expression of resistance in mecA-carrying, low-level methicillin-resistant Staphylococcus haemolyticus. Antimicrobial Agents and Chemotherapy 2001; 45: 19551963.
19.Kobayashi, N, Alam, MM, Urasawa, S. Genomic rearrangement of the mec regulator region mediated by insertion of IS431 in methicillin-resistant staphylococci. Antimicrobial Agents and Chemotherapy 2001; 45: 335338.
20.Kobayashi, N, et al. Distribution of insertion sequence-like element IS1272 and its position relative to methicillin resistance genes in clinically important staphylococci. Antimicrobial Agents and Chemotherapy 1999; 43: 27802782.
21.Suzuki, E, et al. Distribution of mec regulator genes in methicillin-resistant Staphylococcus clinical strains. Antimicrobial Agents and Chemotherapy 1993; 37: 12191226.
22.Zhang, K, et al. Novel staphylococcal cassette chromosome mec type, tentatively designated type VIII, harboring class A mec and type 4 ccr gene complexes in a Canadian epidemic strain of methicillin-resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 2009; 53: 531540.
23.Shore, A, et al. Seven novel variants of the staphylococcal chromosomal cassette mec in methicillin-resistant Staphylococcus aureus isolates from Ireland. Antimicrobial Agents and Chemotherapy 2005; 49: 20702083.
24.Ito, T, et al. Insights on antibiotic resistance of Staphylococcus aureus from its whole genome: genomic island SCC. Drug Resistance Updates 2003; 6: 4152.
25.Kwon, N, et al. Staphylococcal cassette chromosome mec (SCCmec) characterization and molecular analysis for methicillin-resistant Staphylococcus aureus and novel SCCmec subtype IVg isolated from bovine milk in Korea. Journal of Antimicrobial Chemotherapy 2005; 56: 624632.
26.Ma, XX, et al. Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains. Antimicrobial Agents and Chemotherapy 2002; 46: 11471152.
27.Ito, T, et al. Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 2001; 45: 13231336.
28.Katayama, Y, et al. Identification in methicillin-susceptible Staphylococcus hominis of an active primordial mobile genetic element for the staphylococcal cassette chromosome mec of methicillin-resistant Staphylococcus aureus. Journal of Bacteriology 2003; 185: 27112722.
29.Katayama, Y, et al. Jumping the barrier to beta-lactam resistance in Staphylococcus aureus. Journal of Bacteriology 2003; 185: 54655472.
30.Luong, TT, et al. Type 1 capsule genes of Staphylococcus aureus are carried in a staphylococcal cassette chromosome genetic element. Journal of Bacteriology 2002; 184: 36233629.
31.Mongkolrattanothai, K, et al. Novel non-mecA-containing staphylococcal chromosomal cassette composite island containing pbp4 and tagF genes in a commensal staphylococcal species: a possible reservoir for antibiotic resistance islands in Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 2004; 48: 18231836.
32.Chongtrakool, P, et al. Staphylococcal cassette chromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus strains isolated in 11 Asian countries: a proposal for a new nomenclature for SCCmec elements. Antimicrobial Agents and Chemotherapy 2006; 50: 10011012.
33.Hiramatsu, K, et al. The emergence and evolution of methicillin-resistant Staphylococcus aureus. Trends in Microbiology 2001; 9: 486493.
34.Ito, T, et al. Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccrC. Antimicrobial Agents and Chemotherapy 2004; 48: 26372651.
35.Okuma, K, et al. Dissemination of new methicillin-resistant Staphylococcus aureus clones in the community. Journal of Clinical Microbiology 2002; 40: 42894294.
36.Machado, AB, et al. Distribution of staphylococcal cassette chromosome mec (SCCmec) types I, II, III and IV in coagulase-negative staphylococci from patients attending a tertiary hospital in southern Brazil. Journal of Medical Microbiology 2007; 56: 13281333.
37.Ruppé, E, et al. Diversity of staphylococcal cassette chromosome mec structures in methicillin-resistant Staphylococcus epidermidis and Staphylococcus haemolyticus strains among outpatients from four countries. Antimicrobial Agents and Chemotherapy 2009; 53: 442449.
38.Jamaluddin, TZ, et al. Extreme genetic diversity of methicillin-resistant Staphylococcus epidermidis strains disseminated among healthy Japanese children. Journal of Clinical Microbiology 2008; 46: 37783783.
39.Ibrahem, S, et al. Carriage of methicillin-resistant staphylococci and their SCCmec types in a long-term-care facility. Journal of Clinical Microbiology 2009; 47: 3237.
40.Hanssen, AM, Kjeldsen, G, Sollid, JU. Local variants of staphylococcal cassette chromosome mec in sporadic methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci: evidence of horizontal gene transfer? Antimicrobial Agents and Chemotherapy 2004; 48: 285296.
41.Wisplinghoff, H, et al. Related clones containing SCCmec type IV predominate among clinically significant Staphylococcus epidermidis isolates. Antimicrobial agents and Chemotherapy 2003; 47: 35743579.
42.Pi, B, et al. Distribution of the ACME-arcA gene among meticillin-resistant Staphylococcus haemolyticus and identification of a novel ccr allotype in ACME-arcA-positive isolates. Journal of Medical Microbiology 2009; 58(Pt 6): 731736.
43.Li, M, et al. Molecular characterization of Staphylococcus epidermidis strains isolated from a teaching hospital in Shanghai, China. Journal of Medical Microbiology 2009; 58: 456461.
44.Söderquist, B, Berglund, C. Methicillin-resistant Staphylococcus saprophyticus in Sweden carries various types of staphylococcal cassette chromosome mec (SCCmec). Clinical Microbiology Infection (in press).
45.Higashide, M, et al. Methicillin-resistant Staphylococcus saprophyticus isolates carrying staphylococcal cassette chromosome mec have emerged in urogenital tract infections. Antimicrobial Agents and Chemotherapy 2008; 52: 20612068.
46.Yasuda, R, et al. Methicillin-resistant coagulase-negative staphylococci isolated from healthy horses in Japan. American Journal of Veterinary Research 2000; 61: 14511455.
47.Malik, S, et al. Molecular typing of methicillin-resistant staphylococci isolated from cats and dogs. Journal of Antimicrobial Chemotherapy 2006; 58: 428431.
48.Zhang, Y, Agidi, S, Lejeune, JT. Diversity of staphylococcal cassette chromosome in coagulase-negative staphylococci from animal sources. Journal of Applied Microbiology 2009; 107: 1375–83.
49.van Duijkeren, E, et al. Methicillin-resistant staphylococci isolated from animals. Veterinary Microbiology 2004; 103: 9197.
50.Slaughter, DM, et al. Antibiotic resistance in coagulase-negative staphylococci isolated from Cope's gray treefrogs (Hyla chrysoscelis). FEMS Microbiology Letters 2001; 205: 265270.
51.Lee, JH. Methicillin (Oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Applied and Environmental Microbiology 2003; 69: 64896494.
52.Burriel, AR. Resistance of coagulase-negative staphylococci isolated from sheep to various antimicrobial agents. Research in Veterinary Science 1997; 63: 189190.
53.Ziebuhr, W, et al. Nosocomial infections by Staphylococcus epidermidis: how a commensal bacterium turns into a pathogen. International Journal of Antimicrobial Agents 2006; 28 (Suppl. 1): S14S20.
54.Miragaia, M, et al. Comparison of molecular typing methods for characterization of Staphylococcus epidermidis: proposal for clone definition. Journal of Clinical Microbiology 2008; 46: 118129.
55.Miragaia, M, et al. Inferring a population structure for Staphylococcus epidermidis from multilocus sequence typing data. Journal of Bacteriology 2007; 189: 25402552.
56.Musser, JM, Kapur, V. Clonal analysis of methicillin-resistant Staphylococcus aureus strains from intercontinental sources: association of the mec gene with divergent phylogenetic lineages implies dissemination by horizontal transfer and recombination. Journal of Clinical Microbiology 1992; 30: 20582063.
57.Hanssen, AM, Ericson Sollid, JU. SCCmec in staphylococci: genes on the move. FEMS Immunology and Medical Microbiology 2006; 46: 820.
58.Archer, GL, Scott, J. Conjugative transfer genes in staphylococcal isolates from the United States. Antimicrobial Agents and Chemotherapy 1991; 35: 25002504.
59.Forbes, BA, Schaberg, DR. Transfer of resistance plasmids from Staphylococcus epidermidis to Staphylococcus aureus: evidence for conjugative exchange of resistance. Journal of Bacteriology 1983; 153: 627634.
60.Hurdle, JG, et al. In vivo transfer of high-level mupirocin resistance from Staphylococcus epidermidis to methicillin-resistant Staphylococcus aureus associated with failure of mupirocin prophylaxis. Journal of Antimicrobial Chemotherapy 2005; 56: 11661168.
61.Townsend, DE, et al. Conjugative, staphylococcal plasmids carrying hitch-hiking transposons similar to Tn554: intra- and interspecies dissemination of erythromycin resistance. Australian Journal of Experimental Biology and Medical Science 1986; 64: 367379.
62.Udo, EE, Jacob, LE, Mokadas, EM. Conjugative transfer of high-level mupirocin resistance from Staphylococcus haemolyticus to other staphylococci. Antimicrobial Agents and Chemotherapy 1997; 41: 693695.
63.Wielders, CL, et al. In-vivo transfer of mecA DNA to Staphylococcus aureus. Lancet 2001; 357: 16741675.
64.Berglund, C, Söderquist, B. The origin of a methicillin-resistant Staphylococcus aureus isolate at a neonatal ward in Sweden-possible horizontal transfer of a staphylococcal cassette chromosome mec between methicillin-resistant Staphylococcus haemolyticus and Staphylococcus aureus. Clinical Microbiology and Infection 2008; 14: 10481056.
65.Kozitskaya, S, et al. The bacterial insertion sequence element IS256 occurs preferentially in nosocomial Staphylococcus epidermidis isolates: association with biofilm formation and resistance to aminoglycosides. Infection and Immunity 2004; 72: 12101215.
66.Hanssen, AM, Sollid, JU. Multiple staphylococcal cassette chromosomes and allelic variants of cassette chromosome recombinases in Staphylococcus aureus and coagulase-negative staphylococci from Norway. Antimicrobial Agents and Chemotherapy 2007; 51: 16711677.
67.Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; Eighth Informational Supplement, CLSI document M100-S19. Wayne, PA: Clinical and Laboratory Standards Institute, 2009.
68.John, MA, et al. Comparison of three phenotypic techniques for detection of methicillin resistance in Staphylococcus spp. reveals a species-dependent performance. Journal of Antimicrobial Chemotherapy 2009; 63: 493496.
69.Kim, M, et al. Comparison of the MicroScan, VITEK 2, and Crystal GP with 16S rRNA sequencing and MicroSeq 500 v2.0 analysis for coagulase-negative staphylococci. BMC Microbiology 2008; 8: 233.
70.Brigante, G, et al. Identification of coagulase-negative staphylococci by using the BD phoenix system in the low-inoculum mode. Journal of Clinical Microbiology 2008; 46: 38263828.
71.Sampimon, OC, et al. Performance of API Staph ID 32 and Staph-Zym for identification of coagulase-negative staphylococci isolated from bovine milk samples. Veterinary Microbiology 2009; 136: 300305.
72.Clinical Laboratory Standards Institute. Interpretive criteria for microorganism identification by DNA target sequencing; proposed guideline. In: CLSI document MM18-P, Wayne, PA, 2007.
73.Drancourt, M, Raoult, D. rpoB-gene sequence-based identification of Staphylococcus species. Journal of Clinical Microbiology 2002; 40: 13331338.
74.Mellmann, A, et al. Sequencing and staphylococci identification. Emerging Infectious Diseases 2006; 12: 333336.
75.Kwok, AY, et al. Species identification and phylogenetic relationships based on partial HSP60 gene sequences within the genus Staphylococcus. International Journal of Systematic Bacteriology 1999; 49: 11811192.
76.Shah, MM, et al. dnaJ gene sequence-based assay for species identification and phylogenetic grouping in the genus Staphylococcus. International Journal of Systematic and Evolutionary Microbiology 2007; 57: 2530.
77.Capurro, A, et al. Comparison of commercialized phenotyping system, antimicrobial susceptibility testing, and tuf gene sequencebased genotyping for species-level identification of coagulase-negative staphylococci isolated from cases of bovine mastitis. Veterinary Microbiology 2009; 134: 327333.
78.Poyart, C, et al. Rapid and accurate species-level identification of coagulase-negative staphylococci by using the sodA gene as a target. Journal of Clinical Microbiology 2001; 39: 42964301.
79.Becker, K, et al. Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species. Journal of Clinical Microbiology 2004; 42: 49884995.
80.Zhang, K, et al. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. Journal of Clinical Microbiology 2005; 43: 50265033.
81.Pei, Z, et al. Bacterial biota in the human distal esophagus. Proceedings of the National Academy of Sciences USA 2004; 101: 42504255.
82.Echániz-Aviles, G, et al. Molecular characterisation of a dominant methicillin-resistant Staphylococcus aureus (MRSA) clone in a Mexican hospital (1999–2003). Clinical Microbiology and Infection 2006; 12: 2228.
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