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Studies on poliomyelitis virus. Concentration and purification of the virus

Published online by Cambridge University Press:  15 May 2009

A. Polson  and
J. W. F. Hampton
A. Polson
Affiliation:
C.S.I.R. and U.C.T. Virus Research Unit, Department of Pathology, University of Cape Town (South Africa)
J. W. F. Hampton
Affiliation:
Laboratories of the Poliomyelitis Research Foundation, Johannesburg (South Africa)
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For the purpose of determining the immunogenic potency of polio virus, relatively large amounts of concentrated virus material were prepared which had titres of the order of 1010 T.C.I.D.50 per ml. These were obtained by pervaporating large quantities of tissue culture fluid containing approximately 106.5 T.C.I.D.50 per ml.

We wish to acknowledge the valuable assistance received from Dr P. D. Winter and his staff, of the Poliomyelitis Research Foundation, in carrying out the tissue culture titrations of these materials.

Information

Type
Research Article
Information
Journal of Hygiene , Volume 55 , Issue 3 , September 1957 , pp. 344 - 346
Copyright
Copyright © Cambridge University Press 1957

References

Hampton, J. W. F., Polson, A. & Selzer, Golda (1955). Biochim. biophys. Acta, 17, 592.CrossRefGoogle Scholar
Polson, A. & Selzer, G. (1954). Biochim. biophys. Acta, 14, 67.CrossRefGoogle Scholar