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Surveys of human enterotoxigenic Escherichia coli from three different geographical areas for possible colonization factors

Published online by Cambridge University Press:  19 October 2009

M. M. McConnell ,
M. L. Hibberd ,
M. E. Penny ,
S. M. Scotland ,
T. Cheasty  and
B. Rowe
M. M. McConnell
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, Colindale, London, NW9 5HT
M. L. Hibberd
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, Colindale, London, NW9 5HT
M. E. Penny
Affiliation:
Instituto de Investigacion Nutricional, Apartado 18–0191, Miraflores, Lima 18, Peru
S. M. Scotland
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, Colindale, London, NW9 5HT
T. Cheasty
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, Colindale, London, NW9 5HT
B. Rowe
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, Colindale, London, NW9 5HT
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Enterotoxigenic Escherichia coli (ETEC) from Burma, central Africa (Rwanda and Zaire) and Peru, were screened by enzyme-linked immunoassays for the colonization factor antigens (CFAs) and putative colonization factors (PCFs): CFA/I, CFA/II, which consists of three coli surface-associated (CS) antigens, CS1, CS2 and CS3, CFA/III, CFA/IV (CS4, CS5, CS6), CS7, PCFO9, PCFO159.H4, PCFO166, and CS17. The highest proportion of ETEC with identifiable colonization factors (71%) were found in the strains from Burma, which were mainly positive for CFA/I (38%), but strains producing CFA/II (4%), CFA/IV (11%), CS7 (10%), CS17 (4%), PCFO159.H4 (2%) and PCFO166 (2%) were also found. Sixty-nine percent of the ETEC from central Africa were positive for known colonization factors. While CFA/I positive strains were important (12%), a higher number of ETEC producing CFA/IV (33%) and CS17 (24%) were found. Fifty-two percent of the Peruvian strains produced identifiable colonization factors. The largest group of strains produced antigens of the CFA/IV complex (17%), while ETEC producing CFA/II (6%), CFA/III and CS6 (2%), CS7 (6%), PCFO9 (6%), PCFO166 (8%) and CS17 (7%) were also found. These surveys show that there is a considerable variation in the proportions and types of colonization factor found in different geographical areas. From 29 to 48% of the ETEC did not possess an identifiable colonization factor. These were particularly of the LT only producing type. These results have important implications for vaccine formulation.

Type
Research Article
Information
Epidemiology & Infection , Volume 106 , Issue 3 , June 1991 , pp. 477 - 484
Copyright
Copyright © Cambridge University Press 1991

References

REFERENCES

1.Mooi, FR, de Graaf, FK.Molecular biology of fimbriae of enterotoxigenic Escherichia coli. Curr Top Microbiol Immunol 1985; 118: 119–38.Google Scholar
2.Evans, DG, Silver, RP, Evans, DJ JrChase, DG, Gorbach, SL. Plasmid-controlled colonization factor associated with virulence in Escherichia coli enterotoxigenic for humans. Infect Immun 1975; 12: 656–67.Google Scholar
3.Smyth, CJ. Two mannose-resistant haemagglutinins on enterotoxigenic Escherichia coli of serotype O6.K15.H16 or H- isolated from travellers' and infantile diarrhoea. J Gen Microbiol 1982; 128: 2081–96.Google ScholarPubMed
4.Cravioto, A, Scotland, SM, Rowe, B.Hemagglutination activity and colonization factor antigens I and II in enterotoxigenic and non-enterotoxigenic strains of Escherichia coli isolated from humans. Infect Immun 1982; 36: 189–97.Google Scholar
5.Thomas, LV, McConnell, MM, Rowe, B, Field, AM. The possession of three novel coli surface antigens by enterotoxigenic Escherichia coli strains positive for the putative colonization factor PCF8775. J Gen Microbiol 1985; 131: 2319–26.Google Scholar
6.McConnell, MM, Thomas, LV, Scotland, SM, Rowe, B. The possession of coli surface antigen CS6 by enterotoxigenic Escherichia coli of serogroups 025, 027, 0148 and 0159: a possible colonization factor. Curr Microbiol 1986; 14: 51–4.CrossRefGoogle Scholar
7.Honda, T, Arita, M, Miwatani, T. Characterization of new hydrophobic pili of human enterotoxigenic Escherichia coli: a possible new colonization factor. Infect Immun 1984; 43: 959–65.CrossRefGoogle ScholarPubMed
8.Tacket, CO, Maneval, DR, Levine, MM. Purification, morphology and genetics of a new fimbrial putative colonization factor of enterotoxigenic Escherichia coli 0159.H4. Infect Immun 1987; 55: 1063–9.Google Scholar
9.McConnell, MM, Chart, H, Field, AM, Hibberd, M, Rowe, B. Characterization of a putative colonization factor (PCFO166) of enterotoxigenic Escherichia coli of serogroup 0166. J Gen Microbiol 1989; 135: 1135–44.Google Scholar
10.McConnell, MM, Hibberd, M, Field, AM, Chart, H, Rowe, B. Characterization of a new putative colonization factor (CS17) from a human enterotoxigenic Escherichia coli of serotype O114.H21 which produces only heat-labile enterotoxin. J Infect Dis 1990; 161: 343–7.CrossRefGoogle ScholarPubMed
11.Deneke, CF, Thorne, GM, Gorach, SL. Serotypes of attachment pili of enterotoxigenic Escherichia coli isolated from humans. Infect Immun 1981; 32: 1254–60.Google Scholar
12.Hibberd, ML, McConnell, MM, Field, AM, Rowe, B. The fimbriae of human enterotoxigenic Escherichia coli strain 334 are related to CS5 fimbriae. J Gen Microbiol 1990; 136: 2449–56.Google Scholar
13.Heuzenroeder, MW, Elliot, TR, Thomas, CJ, Halter, R, Manning, PA. A new fimbrial type (PCF09) on enterotoxigenic Escherichia coli O9.H-LT+ isolated from a case of infant diarrhea in Central Australia. FEMS Microbiol Lett 1990; 66: 55–60.Google Scholar
14.McConnell, MM, Thomas, LV, Day, NP, Rowe, B. Enzyme-linked immunosorbent assays for detection of adhesion factor antigens of enterotoxigenic Escherichia coli. J Infect Dis 1985; 152: 1120–7.Google Scholar
15.McConnell, MM, Rowe, B. Prevalence of the putative colonization factors CFA/III and PCFO159.H4 in enterotoxigenic Escherichia coli. J Infect Dis 1989; 159: 582–6.CrossRefGoogle ScholarPubMed
16.Ahren, CM, Gothefors, L, Stoll, BJ, Salek, MA, Svennerholm, A-M. Comparison of methods for detection of colonization factor antigens on enterotoxigenic Escherichia coli. J Clin Microbiol 1986; 23: 586–91.CrossRefGoogle ScholarPubMed
17.Changchawalit, S, Echeverria, P, Taylor, DN, Leksomboon, U, Tirapat, C, Eampokalap, B, Rowe, B. Colonization factors associated with enterotoxigenic Escherichia coli isolated in Thailand. Infect Immun 1984; 45: 525–7.Google Scholar
18.Evans, DG, Graham, DY, Evans, DJ JrOpekun, A. Administration of purified colonization factor antigens (CFA/I, CFA/II) of enterotoxigenic Escherichia coli to volunteers. Response to challenge with virulent enterotoxigenic Escherichia coli. Gastroenterology 1984; 87: 934–40.Google Scholar
19.Levine, MM, Morris, JG, Losonsky, G, Boedeker, E, Rowe, B. Fimbriae (pili) adhesins as vaccines. In: D, Lark, Normark, S, Brent-Uhlin, E, eds. Molecular biology of microbial pathogenicity. London: Academic Press, 1986; 143–5.Google Scholar
20.Svennerholm, A-M, LopezVidal, Y, Holmgren, J, McConnell, MM, Rowe, B. Role of PCF8775 antigen and its coli surface subcomponents for colonization, disease and protective immunogenicity of enterotoxigenic E. coli in rabbits. Infect Immun 1988; 56: 523–8.Google Scholar
21.Evans, DG, Evans, DJ JrTjoa, WS, Dupont, HL. Detection and characterization of colonization factor of enterotoxigenic Escherichia coli isolated from adults with diarrhoea. Infect Immun 1978: 19: 727–36.CrossRefGoogle Scholar
22.Ørskov, I, Ørskov, F. Special O:K:H serotypes among enterotoxigenic E. coli strains from diarrhea in adults and children. Mol Microbiol Immunol 1977; 163: 99–110.Google Scholar
23.Aguero, ME, Reyes, L, Prado, V, Ørskov, I, Ørskov, F, Cabello, FC. Enterotoxigenic Escherichia coli in a population of infants with diarrhea in Chile. J Clin Microbiol 1985; 22: 570–81.Google Scholar
24.Thomas, LV, Rowe, B. The occurrence of colonisation factors (CFA/I, CFA/II and E8775) in enterotoxigenic Escherichia coli from various countries in South East Asia. Med Microbiol Immunol 1982; 171: 85–90.CrossRefGoogle ScholarPubMed
25.Gross, RJ, Rowe, B. Serotyping of Escherichia coli. In: M, Sussman, ed. The virulence of Escherichia coli. Reviews and methods. London: Academic Press, 1985; 345–63.Google Scholar
26.Dean, AG, Ching, Y-C, Williams, RG, Harden, LB. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis 1972; 125: 407–11.Google Scholar
27.Donta, ST, Moon, HW, Whipp, SC. Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture. Science 1974; 183: 334–6.Google Scholar
28.McConnell, MM, Thomas, LV, Willshaw, GA, Smith, HR, Rowe, B. Genetic control and properties of coli surface antigens of colonization factor antigen IV (PCF8775) of enterotoxigenic Escherichia coli. Infect Immun 1988; 56: 1974–80.Google Scholar
29.McConnell, MM, Smith, HR, Willshaw, GA, Field, AM, Rowe, B. Plasmids coding for colonization factor antigen 1 and heat-stable enterotoxin production isolated from enterotoxigenic Escherichia coli: comparison of their properties. Infect Immun 1981; 32: 927–36.Google Scholar
30.Smith, HR, Scotland, SM, Rowe, B. Plasmids that code for production of colonization factor antigen II and enterotoxin production in strains of Escherichia coli. Infect Immun 1983; 40: 1236–39.Google Scholar
31.Thomas, LV, Rowe, B, McConnell, MM. In strains of Escherichia coli 0167 a single plasmid encodes for the coli surface antigens CS5 and CS6 of putative colonization factor PCF8775, heat-stable enterotoxin and colicin la. Infect Immun 1987; 55: 1929–31.Google Scholar
32.Cravioto, A, Reyes, RE, Ortega, R, Fernandez, G, Hernandez, R, Lopez, D. Prospective study of diarrhoeal disease in a cohort of rural Mexican children: incidence and isolated pathogens during the first two years of life. Epidemiol Infect 1988; 101: 123–34.Google Scholar
33.Reis, MLH, Matos, DP, Pestana de Castro, AF, Toledo, RF, Trabulsi, RL. Relationship among enterotoxigenic phenotypes, serotypes and sources of strains in enterotoxigenic Escherichia coli. Infect Immun 1980; 28: 24–7.Google Scholar
34.Levine, MM, Nalin, DR, Hoover, DL, Berquist, EJ, Hornick, RB, Young, CR. Immunity to enterotoxigenic Escherichia coli. Infect Immun 1979; 23: 729–36.Google Scholar
35.Clemens, JD, Sack, DA, Harris, JR et al. Cross-protection by B subunit-whole cell cholera vaccine against diarrhea associated with heat-labile toxin-producing enterotoxigenic Escherichia coli: results of a large-scale field trial. J Infect Dis 1988; 158: 372–7.Google Scholar