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Western blot analysis of virus-specific antibody responses for capripox and contagious pustular dermatitis viral infections in sheep

Published online by Cambridge University Press:  15 May 2009

P. Chand ,
R. P. Kitching  and
D. N. Black
P. Chand
Affiliation:
Institute for Animal Health, Pirbright Laboratory, Pirbright, Woking, Surrey, GU24 0NF, England Dept of Veterinary Pathology, College of Veterinary Sciences, C.C.S. Haryana Agricultural University, Hisar 125004, INDIA
R. P. Kitching
Affiliation:
Institute for Animal Health, Pirbright Laboratory, Pirbright, Woking, Surrey, GU24 0NF, England
D. N. Black*
Affiliation:
Institute for Animal Health, Pirbright Laboratory, Pirbright, Woking, Surrey, GU24 0NF, England
*
* Author for correspondence.
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Summary

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This paper reports the development and evaluation of serological tests for the differentiation of antibodies in animals infected with capripox and parapox viruses. Agar-gel immunodiffusion tests using sera from sheep with naturally-acquired infections and from sheep experimentally inoculated with orf or capripox viruses showed cross reactions. Virus-specific antibody responses to structural proteins of the viruses were analysed by Western-blot analysis. This analysis readily differentiated the infections as either capripox or contagious pustular dermatitis. The antibody responses to the 32 kDa and 26 kDa proteins of capripox virus provided a firm basis for differention.

Type
Research Article
Information
Epidemiology & Infection , Volume 113 , Issue 2 , October 1994 , pp. 377 - 385
Copyright
Copyright © Cambridge University Press 1994

References

REFERENCES

1.Dubey, SC, Sawhney, ANSerological relationship between viruses of goat-pox and contagious pustular dermatitis. Indian J Anim Sci 1979; 46: 135–9.Google Scholar
2.Sharma, SN, Dhanda, MRStudies on sheep and goat-pox viruses: Relationship with contagious pustular dermatitis and vaccinia viruses. Indian J Anim Sci 1971; 41: 864–7.Google Scholar
3.Subba Rao, MW and Malik, BSCross-neutralization tests on sheep pox, goatpox and contagious pustular dermatitis viruses. Acta Virol 1979; 23: 165–7.Google Scholar
4.Subba Rao, MV, Malik, BS, Sharma, SNAntigenic relationships among sheep pox, goatpox and contagious pustular dermatitis viruses. Acta Virol 1984; 28: 380–7.Google Scholar
5.Robinson, AJ, Balassu, TContagious pustular dermatitis (orf). Vet Bull 1981; 51: 771–82.Google Scholar
6.Nagington, J, Newton, AA, Horne, RWThe structure of orf virus. Virology 1964; 23: 461–72.CrossRefGoogle ScholarPubMed
7.Wittek, RM, Kuenzle, CC, Wyler, RHigh G-C content in parapoxvirus DNA. J Gen Virol 1979; 43: 231–4.CrossRefGoogle ScholarPubMed
8.Kitching, RP, Hammond, JM, Black, DNStudies on the major common precipitating antigen of capripox. J Gen Virol 1986; 67: 139–48.CrossRefGoogle Scholar
9. Anonymous. Middle East countries refuse “sick” sheep. New Scientist 1989; 124 (No: 1686): 24.Google Scholar
10.Esposito, JJ, Obijeski, JF, Nakano, JHOrthopoxvirus DNA: Strain differentiation by electrophoresis of restriction endonuclease fragmented virion DNA. Virology 1978; 89: 53–66.CrossRefGoogle ScholarPubMed
11.Robinson, AJ, Ellis, G, Balassu, TThe genome of orf virus: Restriction endonuclease analysis of viral DNA isolated from lesions of Orf in sheep. Arch Virol 1982; 71: 43–55.CrossRefGoogle ScholarPubMed
12.Joklik, WKThe purification of four strains of poxvirus. Virology 1962; 18: 9–18.CrossRefGoogle ScholarPubMed
13.Lee, C, Levin, A, Branton, DCopper staining: A five-minute protein stain for sodium dodecyl sulfate-polyacrylamide gels. Anal Biochem 1987; 166: 308–12.CrossRefGoogle ScholarPubMed
14.Hallow, E, Lane, DAntibodies: a laboratory manual. New York: Cold Spring Harbour Laboratory, 1988: 65.Google Scholar
15.Laemmli, UKCleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680–5.CrossRefGoogle ScholarPubMed