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M13 DNA fingerprinting in unicellular and filamentous green algae

Published online by Cambridge University Press:  01 May 1997

BIRGIT OPPERMANN ,
PETR KARLOVSKY  and
WERNER REISSER
BIRGIT OPPERMANN
Affiliation:
Pflanzenphysiologisches Institut der Universität Göttingen, Untere Karspüle 2, D-37073 Göttingen, Germany
PETR KARLOVSKY
Affiliation:
Institut für Phytomedizin der Universität Hohenheim, Otto-Sander-Strasse 5, D-70599 Stuttgart, Germany
WERNER REISSER
Affiliation:
Institut für Botanik der Universität Leipzig, Johannisallee 21–23, D-04103 Leipzig, Germany
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Abstract

The total DNA of ten isolates of different chlorophycean algal genera was extracted according to a protocol for the extraction of fungal DNA. Algal DNA was digested with up to ten different restriction endonucleases. In Southern hybridizations, DNA was probed with a digoxigenin-labelled 440 base pair (bp) fragment of bacteriophage M13 containing a repetitive sequence with a 15 bp core repeat. Stringency conditions for the detection of banding patterns in algae of different genera are described. The stability of DNA fingerprints of subcultures of the same isolate was investigated. DNA fingerprints of isolates of the genera Chlorella, Klebsormidium and Stichococcus were compared. The results show that the M13 minisatellite probe can be used to detect DNA fingerprints in chlorophyte algae of different genera and to differentiate isolates of the same species.

Keywords

algaeDNA fingerprintingM13minisatelliteStichococcus
Type
Research Article
Information
European Journal of Phycology , Volume 32 , Issue 2 , May 1997 , pp. 103 - 110
Copyright
© 1997 British Phycological Society

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