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Molecular characterization of sweet cherry genetic resources inGiresun, Turkey

Published online by Cambridge University Press:  24 January 2011

Taki Demir ,
Leyla Demirsoy ,
Hüsnü Demirsoy ,
Yıldız Aka Kaçar ,
Muharrem Yılmaz  and
Idris Macit
Taki Demir*
Affiliation:
Sakarya Univ., Vocat. Sch. Geyve, 54700, Geyve, Sakarya, Turkey,
Leyla Demirsoy
Affiliation:
Ondokuz Mayıs Univ., Fac. Agric., Dep. Hortic., Samsun, Turkey
Hüsnü Demirsoy
Affiliation:
Ondokuz Mayıs Univ., Fac. Agric., Dep. Hortic., Samsun, Turkey
Yıldız Aka Kaçar
Affiliation:
Cukurova Univ., Fac. Agric., Dep. Hortic., Adana 01330, Turkey
Muharrem Yılmaz
Affiliation:
Cukurova Univ., Fac. Agric., Dep. Hortic., Adana 01330, Turkey
Idris Macit
Affiliation:
Karadeniz Agric. Res. Inst., Samsun, Turkey
*
∗Correspondence and reprints
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Abstract

Introduction. Turkey potentially has a very rich source of sweet(Prunus avium) and sour (P. cerasus) cherries.P. avium is apparently native to some parts of Northern Turkey, whereGiresun is located. Identification of the sweet cherry cultivars produced in Turkey willhelp in choosing appropriate cultivars and aid in the preservation of natural resourcesrequired for breeding studies. The most conventional method of cultivar identification isbased on the assessment of morphological characteristics. However, this method isinsufficient to distinguish closely related cultivars. The aims of our study were todetermine the molecular profile of sweet cherry accessions grown in Giresun, Turkey, andto determine their genetic relationships. Materials and methods. In ourstudy, we identified 44 sweet cherry accessions grown in Giresun by using genetic markers(SSR, Simple Sequence Repeat), and we determined the genetic relationships among the sweetcherry genotypes. For DNA isolation, we collected young leaves sampled on a single plantper accession, then amplification of microsatellite loci was performed. In total, ten SSRprimer pairs, previously isolated from peach and sweet cherry, were used. Geneticsimilarity values were calculated. A cluster analysis was performed to generate adendrogram. Results and discussion. Of the ten primers tested, six primerpairs did not result in suitable amplification products with the 44 accessions studied.The remaining four polymorphic SSR primer pairs produced 33 alleles with an average of8.25 putative alleles per locus, ranging from 7 to 11. Depending on the accessions,similarity ratios ranged from 0.32 to 0.98, with a mean value of 0.64. In conclusion, theresults obtained demonstrate a high level of polymorphism among sweet cherry genotypesfrom a single province in Turkey.

Keywords

TurkeyPrunus aviumgenetic resourcesidentificationmicrosatellitesgenetic markersgenetic distanceTurquiePrunus aviumressource génétiqueidentificationmicrosatellitemarqueur génétiquedistance génétiqueTurquíaPrunus aviumrecursos genéticosidentificaciónmicrosatélitesmarcadores genéticosdistancia genética

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Type
Original article
Information
Fruits , Volume 66 , Issue 1 , January 2011 , pp. 53 - 62
Copyright
© 2011 Cirad/EDP Sciences

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References

Références

Özbek S., Special fruit growing, Ankara Univ. Press., Ankara, Turkey, 1978.
Kaçar Y.A., Classification of important sweet cherry (Prunus avium L.) and sour cherry (Prunus cerasus L.) cultivars and types growing in turkey by DNA fingerprinting methods, Çukurova Univ., thesis, Adana, Turkey, 2001, 190 p.
Demirsoy, L., Demirsoy, H., Characteristics of some local and standard sweet cherry cultivars grown in Turkey, J. Am. Pom. Soc. 57 (2003) 128–136. Google Scholar
Kaçar, Y.A., Iezzoni, A.F., Çetiner, M.S., Sweet cherry cultivar identification by using SSR markers, J. Biol. Sci. 5 (2005) 616–619. Google Scholar
Kaçar, Y.A., Çetiner, M.S., Cantini, C., Iezzoni, A.F., Simple sequence repeat (SSR) markers differentiate Turkish sour cherry germplasm, J. Am. Pomol. Soc. 60 (2006) 136–143. Google Scholar
Öz M.H., Aygün H., Soydam S., Çukadar K., Bakır M., Ünlü H.M., Karadoan B., Vurgun H., Ergül A., Molecular analysis of plum and sweet cherry genetic resources in east Anatolia by SSR, V. Natl. Hortic. Congr., Erzurum, Turkey, 2006, poster.
Browicz K., Prunus, in: Davis P.H. (Ed.), Flora of Turkey and East Aegean Island, Vol. 4, Edinb. Univ. Press, Edinb., U.K., 1972, 8–12.
Beaver, J.A., Iezzoni, A.F., Ramm, C.W., Isozyme diversity in sour, sweet and ground cherry, Theor. Appl. Genet. 90 (1995) 847–852. CrossRefGoogle ScholarPubMed
Boskovic, R., Tobbut, K.R., Nicoll, F.J., Inheritance of isoenzymes and their linkage relationships in two interspecific cherry progenies, Euphytica 93 (1997) 129–143. CrossRefGoogle Scholar
Zhou, L., Kappel, F., Wiersma, P.A., Hampson, C., Bakkeren, G., Genetic analysis and DNA fingerprinting of sweet cherry cultivars and selections using Amplified Fragment Length Polymorphisms (AFLP), Acta Hortic. 667 (2005) 37–44. CrossRefGoogle Scholar
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V., DNA polymorphisms amplified by arbitrary primers are useful as genetic markers, Nucleic Acid Res. 18 (1990) 6531–6535. CrossRefGoogle ScholarPubMed
Yang H., Schmitt H., Selection of a mutant from adventitious shoots formed in X-ray treated leaves and differentiation of standard from mutant with RAPDs, in: Schmith H., Kellerhals M. (Eds.), Progress in temperate fruit breeding, Kluwer Acad. Publ., Dordrecht, Neth., 1994, pp. 287–290.
Corona M.G., Resta P., Fanizza G., Palasciano M., Godini A., Prima indagine su cultivar pugliesi di ciliegio dolce (Prunus avium L.) mediante lo studio di polimorfismi del DNA (RAPDs), in: Godini A. (Eds.), Atti del Covegno Nazionale del Ciliegio, Valezano (Ba), Italy, 1997, pp. 201–213.
Gerlach, H.K., Stösser, R., Sweet cherry cultivar identification using RAPD derived DNA fingerprints, Acta Hortic. 468 (1998) 63–70. CrossRefGoogle Scholar
Demirsoy, L., Demir, T., Demirsoy, H., Kaçar, Y.A., Okumu, A., Identification of some sweet cherry cultivars grown in Amasya by RAPD markers, Acta Hortic. 795 (2008) 147–153. CrossRefGoogle Scholar
Boritzki, M., Plieske, J., Struss, D., Cultivar identification in sweet cherry (Prunus avium L.) using AFLP and microsatellite markers, Acta Hortic. 538 (2000) 505–510. CrossRefGoogle Scholar
Downey, S.L., Iezzoni, A.F., Polymorphic DNA markers in black cherry (Prunus serotina) are identified using sequences from sweet cherry, peach and sour cherry, J. Am. Soc. Hortic. Sci. 125 (2000) 76–80. Google Scholar
Struss, D., Ahmad, R., Southwick, S.M., Boritzki, M., Analysis of sweet cherry (Prunus avium L.) cultivars using SSR and AFLP markers, J. Am. Soc. Hortic. Sci. 128 (2003) 904–909. Google Scholar
Tavaud, M., Zanetto, A., Santi, F., Dirlewanger, E., Structuration of genetic diversity in cultivated and wild cherry varieties using molecular markers, Acta Hortic. 546 (2001) 263–269. CrossRefGoogle Scholar
Sosinski, B., Gannavarapu, M., Hager, L.D., Beck, L.E., King, G.J., Ryder, C.D., Rajapakse, S., Baird, W.V., Ballard, R.E., Abott, A.G., Characterization of microsatellite markers in peach, Theor. Appl. Genet. 97 (2000) 264–272. Google Scholar
Cantini, C., Iezzoni, A.F., Lamboy, W., Boritzki, M., Struss, D., DNA fingerprinting of tetraploid cherry germplasm using simple sequence repeats, J. Am. Soc. Hortic. Sci. 126 (2001) 205–209. Google Scholar
Nei M., Li W., Mathematical model for studying genetic variance in terms of restriction endonucleases, Proc. Natl. Acad. Sci. 76