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Protein and amino acid profiles of Tunisian Deglet Nour and Allig date palm fruit seeds

Published online by Cambridge University Press:  24 January 2008

Mohamed Ali Bouaziz ,
Souhail Besbes ,
Christophe Blecker ,
Bernard Wathelet ,
Claude Deroanne  and
Hamadi Attia
Mohamed Ali Bouaziz
Affiliation:
Unité Analyses Alimentaires, École Nationale d’Ingénieurs de Sfax, Route de Soukra, 3038 Sfax, Tunisia
Souhail Besbes
Affiliation:
Unité Analyses Alimentaires, École Nationale d’Ingénieurs de Sfax, Route de Soukra, 3038 Sfax, Tunisia
Christophe Blecker
Affiliation:
Unité de Technologie des Industries Agro-alimentaires, Faculté Universitaire des Sciences Agronomiques de Gembloux, passage des Déportés 2, 5030 Gembloux, Belgium
Bernard Wathelet
Affiliation:
Unité de Chimie Biologique Industrielle, Faculté Universitaire des Sciences Agronomiques de Gembloux, passage des Déportés 2, 5030 Gembloux, Belgium
Claude Deroanne
Affiliation:
Unité de Technologie des Industries Agro-alimentaires, Faculté Universitaire des Sciences Agronomiques de Gembloux, passage des Déportés 2, 5030 Gembloux, Belgium
Hamadi Attia
Affiliation:
Unité Analyses Alimentaires, École Nationale d’Ingénieurs de Sfax, Route de Soukra, 3038 Sfax, Tunisia
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Abstract

Introduction. The fruit of the date palm (Phoenix dactylifera L.) is an important crop of the arid and semi-arid areas of the world. It has always played a genuine economic and social part in the lives of the people of these areas. Nevertheless, there are few studies related to the composition and the functional properties of the different components (fibres, fats and proteins) of the date seeds. In order to obtain more information on the nutritive value and the chemical composition of the pits of the most important and most valuable Tunisian date palm varieties (Deglet Nour and Allig), we studied their profiles of proteins and amino acids. Materials and methods. Date seeds of two Tunisian cultivars, Deglet Nour and Allig, were separately milled in a heavy-duty grinder to pass 1–2 mm screens; then, they were preserved at – 20 °C until analysis. The soluble proteins from defatted date seeds were extracted with water at pH 10, and were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Results and discussion. Amino acid profiles revealed that date seeds from the Deglet Nour and Allig varieties contained the majority of essential amino acids: lysine, isoleucine, leucine, methionine, threonine, valine and phenylalanine. Seventeen amino acids were detected in the seeds of the two studied cultivars. Glutamic acid was the major amino acid in Deglet Nour and Allig date seeds, representing 17.83% and 16.77%, respectively, of the total amino acids. Date seeds appeared to contain a number of proteins with molecular weights ranging from 22 kDa to 70 kDa. Three prominent bands appeared at 32 kDa, 60 kDa and 70 kDa.

Keywords

TunisiaPhoenix dactyliferafruitsseedschemical compositionproteinsamino acidsTunisiePhoenix dactyliferafruitsgrainecomposition chimiqueprotéineacide aminé
Type
Research Article
Information
Fruits , Volume 63 , Issue 1 , January 2008 , pp. 37 - 43
Copyright
© CIRAD, EDP Sciences, 2008

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References

Al-Obaidi Z.S., Berry D.R., Scaling up of citric acid fermentation using fermenters, Date Palm J. 2 (1982) 19–33.
Almana, H.A., Mahmoud, R.M., Date palm seed as an alternative source of dietary fibre in Saudi bread, Ecol. Food Nutr. 32 (1994) 261–270. CrossRef
Hamada, J.S., Hashim, I.B., Sharif, A.F., Preliminary analysis and potential uses of date pits in foods, Food Chem. 76 (2002) 135–137. CrossRef
Besbes, S., Blecker, C., Deroanne, C., Drira, N.E., Attia, H., Date seeds: chemical composition and characteristic profiles of the lipid fraction, Food Chem. 84 (2004) 577–584. CrossRef
Besbes, S., Hentati, B., Blecker, C., Deroanne, C., Lognay, G., Drira, N.E., Attia, H., Voies de valorisation des sous produits de dattes: valorisation du noyau, Hyg. Microbiol. Aliment. 49 (2005) 1–9.
Gihad, E.A., El-Gallad, T.T., Sooud, A.E., Abdou, E.L., Nasr, H.M., Farid, M.F.A., Feed and water intake, digestibility and nitrogen utilisation by camels compared to sheep and goats fed low protein dessert by-products, CIHAEM-Options Méditerr. Sér. A 2 (1989) 75–81.
Estanvove, P., Valorisation de la datte, Note tech., CIHAEM-Options Méditerr., Sér. A 11 (1990) 301–318.
Elgasim, E.A., Alyousef, Y.A., Humeida, A.M., Possible hormonal activity of date pits and flesh fed to meat animals, Food Chem. 52 (1995) 149–152. CrossRef
Youssif, A.K., Alghamdi, A.S., Hamad, A., Mustafa, A.I., Processing and evaluation of a date juice-milk drink, Egypt. J. Dairy Sci. 24 (1996) 277–288.
Jwanny, E.W., Rashad, M.M., Moharib, S.A., El Beih, N.M., Biological evaluation of date-waste dietary fibre and Endomycopsis fibuligera protein with rats, Bioresour. Technol. 56 (1996) 201–205. CrossRef
Hussein, A.S., Alhadrami, G.A., Khalil, Y.H., The use of dates and date pits in broiler starter and finisher diets, Bioresour. Technol. 66 (1998) 219–223. CrossRef
Belal, I.E.H., Al-Jassar, M.S., Mustafa, I.A., Al-Dosari, M.N., Evaluation of date-feed ingredients mixes, Anim. Feed Sci. Technol. 81 (1999) 291–298. CrossRef
Ali, B.H., Bashir, A.K., Alhadrami, G., Reproductive hormonal status of rats treated with date pits, Food Chem. 66 (1999) 437–441. CrossRef
Myhara, R.M., Byerley, D.J., Mahgoub, O., Chesworth, J.M., Effects of testicular status and feeding diets containing date palm by-product on the sensory attributes of Omani lamb meat, Small Rumin. Res. 38 (2000) 57–61. CrossRef
Aldhaheri, A., Alhadrami, G., Aboalnaga, N., Wasfi, I., Elridi, M., Chemical composition of date pits and reproductive hormonal status of rats fed date pits, Food Chem. 86 (2004) 93–97. CrossRef
Nacib, N., Nacib, A., Bourdant, J., Use of waste products in the fermentative formation of Baker’s yeast biomass by Saccharomyces cervisiae, Bioresour. Technol. 60 (1997) 67–71.
Nacib, N., Nacib, A., Mostefa, G., Larbi, L., Adimi, L., Remmal, M., Bourdant, J., Use of date products in production of thermophilic dairy starters strain Streptococcus thermophilus, Bioresour. Technol. 67 (1999) 291–295.
Abou Zied A.A., Baghlef A.O., Utilization of date seeds and cheese whey in production of citric acid by Candida lipolytica, Agric. Wastes 8 (1983)131–142.
Abou Zied, A.A., Abderrahman, N., Baghlef, A.O., The formation of oxytetracycline in ate-coat medium, Bioresour. Technol. 37 (1991) 179–184.
Abou Zied, A.A., Khoja, S.M., Utilization of dates in the fermentative formation of citric acid by Candida lipolytica, Zent. Microbiol. 148 (1993) 213–221.
Abou Zied, A.A., Baeshin, N.A., Baghlaf, A.O., Utilisation of date products in production of oxytetracycline by Streptomyces rimosus, J. Chem. Technol. Biotechnol. 58 (1993) 77–79.
Roukas, T., Kotzekidou, P., Pretreatment of date syrup to increase citric acid production, Enzym. Microbiol. Technol. 21 (1997) 273–276. CrossRef
Nacib N., Nacib A., Boudjelal A., Benslimane C., Blanchard F., Bourdant J., The effect of supplementation by different nitrogen sources on the production of lactic acid from date juice by Lactobacillus casei subsp. rhamnosus, Bioresour. Technol. 78 (2001) 149–153.
El-Shurafa, M.Y., Ahmed, H.S., Abou-Naji, S.E., Organic and inorganic constituent of dates palm pit (seeds), Date Palm J. 2 (1982) 275–284.
Devshony, S., Eteshola, A., Shani, A., Characterisation and some potential application of date palm (Phoenix dactylifera L.) seeds and seed oil, J. Am. Oil Chem. Soc. 69 (1992) 595–597. CrossRef
Al-Hooti, S., Sidhu, J.S., Qabazard, H., Chemical composition of seeds of date fruit cultivars of United Arab Emirates, J. Food Sci. Technol. 35 (1998) 44–46.
Besbes, S., Blecker, C., Deroanne, C., Lognay, G., Drira, N.E., Attia, H., Quality characteristics and oxidative stability of date seed oil during storage, Food Sci. Technol. Int. 10 (2004) 333–338.
Besbes, S., Blecker, C., Deroanne, C., Bahloul, N., Lognay, G., Drira, N.E., Attia, H., Date seed oil: phenolics, tocopherol and sterol profiles, J. Food Lipids 11 (2004) 251–265. CrossRef
Besbes, S., Blecker, C., Deroanne, C., Lognay, G., Drira, N.E., Attia, H., Heating effects on some quality characteristics of date seed oil, Food Chem. 91 (2005) 469–476. CrossRef
El-Adawy, T.A., Rahma, E.H., El-Bedawey, A.A., Gafar, A.F., Nutritional potential and functional properties of sweet and bitter lupin seed protein isolates, Food Chem. 74 (2001) 455–462. CrossRef
Anon., Official methods of analysis, 15th ed., Assoc. Off. Anal. Chem. (AOAC), Washington, DC, USA, 1995.
Barminas, J.T., James, M.K., Abubakar, U.M., Chemical composition of seeds and oil of Xylopia aethiopica grown in Nigeria, Plant Foods Hum. Nutr. 53 (1999) 193–198. CrossRef
Laemmli, U.K., Cleavage of structural proteins during the assembly of head of bacteriophage T4 , Nature 227 (1970) 680–685. CrossRef
Salem, S.A., Hegazi, S.M., Chemical composition of the Egyptian dry dates, J. Sci. Food Agric. 22 (1971) 632–634. CrossRef
Al-Rawi, N., Markakis, P., Bauer, D.H., Amino acid composition of some Iraqi dates, J. Sci. Food Agric. 18 (1967) 1–2. CrossRef
Salim, S., Ahmed, A., Protein and amino acid contents of some Saudi Arabian date palm seeds (Phoenix dactylifera L.), Arab Gulf J. Sci. Res. 10 (1992) 1–9.
Fayadh, J.M., Al-Showiman, S.S., Chemical composition of date palm (Phoenix dactyli-fera L.), J. Chem. Soc. Pak. 12 (1990) 84–103.
Ahmed, I.S.A., Al-Gharibi, K.N., Daar, A.S., Kabir, S., The composition and properties of date proteins, Food Chem. 53 (1995) 441–446. CrossRef