Introduction. Elaeagnus latifolia Linn. is a type of wildedible fruit found in northeast India, Thailand and also in Vietnam. Although the fruit isreported to be a source of vitamins, minerals, essential fatty acids and other bioactivecompounds, only a few studies have been concerned with the antioxidant activity of thisplant. Materials and methods. Our study revealed in vitroantioxidant and free radical scavenging activity of 70% methanolic extract ofElaeagnus latifolia Linn. (ELME). Various tests includingidentification and quantification of phytochemicals, total antioxidant activity, hydroxylradical, superoxide radical, singlet oxygen, hypochlorous acid scavenging, reducing powerand DNA protection assays were performed. Results and discussion. Among thetests, ELME scavenged superoxide radical [IC50 = (150.78 ± 4.2) μg×mL–1], hydroxyl radical[IC50 = (238.09 ± 11.63) μg×mL–1] and protected pUC18 DNA [P50 = (695.91± 15.84) μg×mL–1]; P50 signifies the concentration for 50% protection . The fruit is foundto be a source of minute amounts of carbohydrates, ascorbic acid, tannins, phenolics andflavonoids. HPLC data showed that purpurin, tannic acid, quercetin, catechin, reserpineand rutin are present in ELME. Conclusion. Our results provide evidence that70% methanol extract of E. latifolia Linn. acts as a promisingantioxidant as well as DNA protector, which is partly due to the phenolic and flavonoidcompounds present in it.